Bio phile element composition and species composition of marine phytoplaskton

海洋真光层浮游植物生物生产的产物（有机物、碳酸钙、蛋白石）是海洋中主要（N、P、Si、Ca等）和次要亲生物元素物质循环的起始物质。这些海洋生物制品的元素组成从主要元素到微量元素的数据相当有限。本研究的目的是：利用专门设计的非金属NORPAC网采集亚北极北太平洋和白令海地区的海洋浮游植物样品，开发一种简单的分步浸出法进行主成分分析，并将主微量元素分馏到各主组分相，获得海洋浮游植物样品的多元素组成，并比较浮游植物样品的元素组成和物种组成。在北太平洋和白令海亚北极海域采集了25 ~ 335 μm硅藻为主的浮游植物样品，建立了一种简单的阶梯浸出法，对海盐、碳酸钙、有机质、欧泊石和残余硅酸铝进行了分析，并测定了这些主要成分中主微量元素的分馏。对这些浮游植物样品的种类组成进行了显微鉴定。结果表明，这些样品的12种主要和微量元素组成在生物相中，并估计了这些元素与生物相的关联。本文所建立的分析方法将成为阐明海洋环境中与生物生产有关的这些元素的行为的有效方法。

Products of biological production by phytoplankton (organic matter, calcium carbonate and opal) in the euphotic layer of the oceans are starting materials from material cycling of both major (N, P, Si, Ca, etc.) and minor biophile elements in the oceans. The data on elemental compositions from major to trace elements of these marine biological products have been quite limited. The purposes of this study are : to collect marine phytoplankton samples from the subarctic north Pacific and Bering Sea area with a specially designed non-metal NORPAC net, to develop a simple stepwize leaching method for major component analysis and for fractionation of major and trace elements into each major component phases, to obtain multi-element compositions of marine phytoplanlkton samples and to compare the elemental composition and species composition of phytoplankton samples.We sampled phytoplankton samples mainly composed of diatom with a size range of 25-335 μm from the subarctic North Pacific and Bering Sea area, developed a simple stepwize leaching method to analyze sea salt, calcium carbonate, organic matter, opal and residual aluminosilicate, determined the fractionation of major and trace elements in to these major components. Species compositions of these phytoplankton samples were identified microscopically. As the results we showed the 12 major and trace element compositions in biogenic phase of these samples and estimated the association of these elements with biogenic phases. The analytical method developed here will become an effective method to clarify the behaviors of these elements associating with biological production in marine environments.