Molecular genetical analysis of Plant gene expression by phytohormones

植物激素植物基因表达的分子遗传学分析

• 批准号: 08454263
• 负责人: SHINOZAKI Kazuo
• 金额: $ 1.73万
• 依托单位: Institute of Physical and Chemical Rescarch (RIKEN)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08454263/
• 关键词: plant hormone; abscisic acid; transcription; transgenic plants; Arabidopsis thaliana; dehydration stress; cis acting elements; trans acting elements; 転写制御因子; 遺伝子発現; MYB; MYC; シロイズナズナ

Under water deficit conditions, various genes are induced and are thought to function in stress tolerance and response. To understand molecular responses to drougt stress, we cloned about 50 cDNAs for drought-inducib1e genes form Arabidopsis thaliana. These drought-inducible genes are classified into two groups, one is function proteins that are involved in drought tolerance and thc other is regulatory proteins involved in stress response. We analyzed the expression patterns of these drought-inducible genes, and identified at least four independent signal transduction pathways between the initial dehydration signal and gene expression. Two are ABA-dependentand two are ABA-independent.We identified a cis-acting elerment (DRE,TACCGACAT) involved in one of the ABA-independent gene expression of the rd29A gene in response to dehydration and cold stress. We isolated and analyzed cDNAs (DREB) for DRE binding proteins by yeast two hybrid system. They are classified in tow two subgroups.They e … More ncode DNA binding proteins that contoin a conserved DNA binding motif found in AP2 and EREBP proteins. DREBl genes are induced by low temperature, while DREB2 genes are induced by drought and high salinity. These results indicate that two DREB proteins, DREBl and DREB2, function as transcription factors in two separate signal transduction pathways under low temperature and dehydration, respectively.The induction of an Arabidopsis drought-inducible genes, rd22, is mediated by ABA,but requires protein biosynthesis for its ABA-dependent expression, A 67-bp region of the rd22 promoter is essential for its ABA-responsive expression, and contains several conserved motifs of DNA binding proteins, such as MYC and MYB,but MYB,but no ABREs. Rccently, a cDNA for a transcription factor MYC homologue was cloned by southwestern method using the 67-bp DNA as a probe, which suggests that drought-inducible MYC homologue may function in the ABA-inducible expression of rd22. These results indicates that drought-or ABA-inducible transcription factor(s) for MYB and MYC function in the ABA-responsive gene expression under drought stress. Less

在缺水条件下，各种基因被诱导，并被认为在逆境耐性和反应中发挥作用。为了了解干旱胁迫的分子响应，我们从拟南芥中克隆了大约50个干旱诱导基因的cDNA。这些干旱诱导基因分为两类，一类是参与抗旱的功能蛋白，另一类是参与胁迫反应的调节蛋白。我们分析了这些干旱诱导基因的表达模式，并确定了在初始脱水信号和基因表达之间至少有四条独立的信号转导途径。两个是ABA依赖的，两个是ABA不依赖的。我们发现了一个顺式作用加速蛋白(DRE，TACCGACAT)，参与了一个ABA不依赖的rd29A基因在脱水和低温胁迫下的表达。我们利用酵母双杂交系统分离和分析了DRE结合蛋白的cDNAs(DREB)。他们被分成两个亚组。他们是…更多的NCode DNA结合蛋白，包含在AP2和EREBP蛋白中发现的保守的DNA结合基序。DREB1基因受低温诱导，而DREB2基因受干旱和高盐诱导。这些结果表明，两个DREB蛋白DREB1和DREB2在低温和脱水条件下分别在两个不同的信号转导途径中发挥转录因子的作用。拟南芥干旱诱导基因rd22的诱导是由ABA介导的，但其依赖于ABA的表达需要蛋白质的生物合成，rd22启动子的一个67bP区域是其ABA应答表达所必需的，并且含有几个保守的DNA结合蛋白基序，如MYC和MYB，MYB，而不是ABRES。以67bp的DNA为探针，用Southwest方法克隆了一个转录因子MYC同源物的cDNA，这表明干旱诱导的MYC同源物可能在ABA诱导的rd22表达中起作用。这些结果表明，干旱或脱落酸诱导转录因子(S)针对MYB和MYC在干旱胁迫下ABA应答基因的表达中发挥作用。较少

项目成果

Tomohiro Kiyosue: "A nuclear gene encoding mitochondrial proline dehydrogenase,an enzyme involved in proline metabolism,is upregulated" The Plant Cell. 8. 1323-1335 (1996)

Tomohiro Kiyosue：“编码线粒体脯氨酸脱氢酶（一种参与脯氨酸代谢的酶）的核基因被上调”《植物细胞》。

K.Shinozaki, and K.Yamaguchi, Shinozaki: "Molecularresponses to drought and cold stress" Current Opinion in Biotech. 7. 161-167 (1996)

K.Shinozaki 和 K.Yamaguchi、Shinozaki：“对干旱和寒冷胁迫的分子反应”生物技术当前观点。

T.Iwasaki, T.Kiyosue, K.Yamaguchi-shinozaki, and K.Shinozaki: "The dehydration-inducibleRd 17 (Cor47) gene and its promotor region in Arabidopsis thaliana (Accession No.AB004872)" Plant Physiol. (PGR 97-155). 115. 1287 (1997)

T.Iwasaki、T.Kiyosue、K.Yamaguchi-shinozaki 和 K.Shinozaki：“拟南芥中脱水诱导的 Rd 17 (Cor47) 基因及其启动子区域（登录号 AB004872）”植物生理学。

Y.Yoshiba, T.Kiyosue, K.Nakashima, K.yamaguchi-shinozaki, and K.Shinozaki: "Regulation of levels of proline as an osmolyte in Plants under water stress" The Plant Cell. 38. 1095-1102 (1997)

Y.Yoshiba、T.Kiyosue、K.Nakashima、K.yamaguchi-shinozaki 和 K.Shinozaki：“水分胁迫下植物中作为渗透剂的脯氨酸水平的调节”植物细胞。

Kazuo Nakashima et al.: "A nuclear gene,erd1,encoding a chloroplast-targeted C1p protease regulatory subunit homolog is not only induced by water stress but also developmetally up-regulated during senescence in Arabidopsis thaliana" The Plant Journal. 12.

Kazuo Nakashima 等人：“编码叶绿体靶向 C1p 蛋白酶调节亚基同源物的核基因 erd1 不仅由水分胁迫诱导，而且在拟南芥衰老过程中金属上调”《植物杂志》。