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Highly sensitive determination in a microscopic region using a near-field microscope.

使用近场显微镜在微观区域进行高灵敏度测定。

基本信息

批准号：
08455389
负责人：
OGAWA Teiichiro
金额：
$ 4.99万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

(1) PurposeIn order to improve a highly sensitive and effective analytical technique in a microscopic region, a new instrument should be constructed based on near-filed and confocal laser-induced fluorescence microscopy. Various application should also be carried out.(2) Results(1) Desigh and construction.///A new laser-induced fluorescence microscope instrument was design and constructed with a purchase of a two-dimensional CCD system using an Argon laser and a diode laser as excitation sources to improve sensitivity and spatial resolution.(2) Optical fiber bundle system.///A unique feature of the new instrument lies in the use of optical fiber bundle. The bundle was circular at the microscope focus to obtain images of a wide region and linear at the slit of the monochromator. This configuration allowed to observe fluorescence at each optical fiber simultaneously on CCD.(3) Image presentation.///The image on CCD allowed direct observation of molecular dynamics on water surface. The molecules stayd on water surface like an island.(4) Fluorescence spectrum of porphyn.///The fluorescence spectra of several porphyns were measured on water surface and found to agree with those in hydrocarbons. Thus, we can conclude that the water surface is as hydrophobic as the bulk of hydrocarbons.(5) Dynamics on water surface.///The time-resolved fluorescence spectra showed some fluctuation, which can be understood by a model that molecules form a cluster on water surface and clusters are moving on it.(6) Fluorescence spectrum of cyanines.///The fluorescence spectra of several cyanines were measured on water surface and agreed with those in hydrocarbons. Thus, the water surface is as hydrophobic as the bulk of hydrocarbons.(7) Conclusion.///The sensitivity has been not as much high as expected due to difficulties in long-time accumulation owing to the Brownian motion.

(1)目的为了在微观领域发展一种高灵敏度、高效率的分析技术，需要构建一种基于近场共聚焦激光诱导荧光显微镜的新仪器。还应进行各种应用。(2)结果（1）设计和施工。为了提高激光诱导荧光显微镜的灵敏度和空间分辨率，采用氩激光器和半导体激光器作为激发光源，通过购买二维CCD系统，设计并构建了一种新型的激光诱导荧光显微镜。(2)光纤束系统/新仪器的一个独特之处在于使用了光纤束。该束在显微镜焦点处是圆形的，以获得宽区域的图像，并且在单色器的狭缝处是线性的。该配置允许在CCD上同时观察每个光纤处的荧光。(3)图片展示// CCD上的图像允许直接观察水面上的分子动力学。分子像一个岛一样停留在水面上。(4)卟啉的荧光光谱测量了几种卟啉化合物在水面上的荧光光谱，发现其荧光光谱与在烃中的荧光光谱一致。因此，我们可以得出结论，水的表面是疏水的，因为大部分的碳氢化合物。(5)水面上的动态。//时间分辨荧光光谱显示出一定的波动，这可以用分子在水面上形成团簇并在其上运动的模型来解释。(6)花青的荧光光谱。///测定了几种菁染料在水面上的荧光光谱，与在烃类中的荧光光谱一致。因此，水表面与烃的主体一样疏水。(7)结论./由于布朗运动导致的长时间积累的困难，灵敏度没有预期的那么高。