Research on Symbiotic Bacteria in Capitella sp.1
Research on Symbiotic Bacteria in Capitella sp.1
批准号:
08456093
负责人:
KOGURE Kazuhiro
金额:
$4.86万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1997
中文摘要
Capitella sp.I是一种底栖生物,其正常栖息地是沿海污染的沉积物。以前的观察表明,这种生物的存在可能会防止环境的进一步恶化。其实际作用机理为上层水柱引入含氧海水,将硫化物氧化成硫磺或硫酸盐。细菌可能是卡皮氏菌的重要营养来源。I和/或环境中有机污染物的降解剂。这项调查的目的是澄清这些贡献。在本研究过程中,我们开发了一种新的培养和观察Capitella sp.行为的方法。I直接,用透明容器和固化琼脂。此外,我们使用了化学物质(INT),这使得我们能够可视化和量化细菌的呼吸活动。结果表明,卡皮氏菌属(Capitella sp.)I通过在沉积物上打洞并引入含氧海水来刺激细菌的活动。由于异养细菌的活性被认为与有机物的降解有关,因此推测Capitella sp.的存在和行为。我确实在防止恶化方面发挥了重要作用。这项新技术将适用于底栖生物与细菌相互作用的各种研究。
英文摘要
Capitella sp. I is a bcnthic organism of which normal habitat is coastal polluted sediments. Previous observations clarified that presence of this organism may prevent from further deterioration of the environments. The actual mechanism was assumed to be introduction of oxic seawater from upper water column and oxidation of sulfide to sulfur or sulfate. Bacteria may play an important role as nutritional source for Capitella sp. I and/or degrader of organic pollutants in the environments. The purpose of this investigation was to clarify these contributions. During the course of this study, we developed a new method to culture and observe the behavior of Capitella sp. I directly, by using transparent containcr and solidified agar. In addition, we used the chemical (I NT) which made it possible to visualize and quantity the bacterial respiratory activity. The result showed that Capitella sp. I stimulates the activity of bacteria by making holes in the sediment and introducing oxic seawater. As heterotrophic bacterial activity is assumed to couple to degradation of organic matter, it was conchided that presence and behavior of Capitella sp. I does play important roles to prevent from deterioration. This new technique will be applicable for various research on the interact on between benthic organisms and bacteria.
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海外基金