Ecological study on marine luminous bacteria possessing proteorhodopsin

具有蛋白视紫红质的海洋发光细菌的生态学研究

• 批准号: 21380123
• 负责人: KOGURE Kazuhiro
• 金额: $ 11.4万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380123/
• 关键词: プロテオロドプシン; 海洋細菌; 光エネルギー; プロトンポンプ活性; Flavobacteria; 遺伝子解析; 遺伝子伝搬; 16S rDNA; Bacteroidetes; エルギー代謝; 海洋生態系; 系統解析; エネルギー代謝

Proteorhodopsin (PR) is a membrane protein that absorbs light energy and functions as proton pump. The proton gradient thus established enables the cells to synthesize ATP, thus serves as efficient energy synthesis component. This was first reported in 2000. Since then there have been estimated that nearly half of prokaryotes present in the upper water column in the ocean may possess PR. However, so far only about 10 isolates had been reported to possess PR, that makes any further physiological investigations difficult. Our group succeeded to isolate more than 60 strains and directly measure the activity of proton pump under light. In addition, with further assumptions we succeeded to estimate the contribution of PR to light energy absorption to the ocean for the first time. The research on PR is now expanding after our investigation using isolates.

前视紫红质（PR）是一种吸收光能并具有质子泵功能的膜蛋白。由此建立的质子梯度使细胞能够合成ATP，从而用作有效的能量合成组分。这是2000年首次报道的。从那时起，有估计，近一半的原核生物存在于上层水体中的海洋可能具有PR。然而，到目前为止，只有大约10个分离物已被报道具有PR，这使得任何进一步的生理调查困难。本课题组成功分离出60多株菌株，并在光照下直接测定了质子泵活性。此外，通过进一步的假设，我们首次成功地估计了PR对海洋光能吸收的贡献。在我们使用分离株进行调查后，对PR的研究正在扩大。

项目成果

Exploring marine microbial unknown functions

探索海洋微生物的未知功能

• 发表时间: 2012
• 作者: 平井慈恵;小磯雅彦;照屋和久;奥澤公一;小林真人;武部孝行;佐藤琢;中村航;後藤敬行;萩原篤志;木暮一啓
• 通讯作者: 木暮一啓

"Photoheterotrophy" -an overlooked mechanism of marine bacteria

“光异养”——海洋细菌被忽视的机制

• 发表时间: 2011
• 作者: Nishizawa;T.;et al.;橘本緑・白木原国雄・白木原美紀;Kogure Kazuhiro
• 通讯作者: Kogure Kazuhiro

Diversity and functional analysis of proteorhodopsin in marine Flavobacteria.

• DOI: 10.1111/j.1462-2920.2012.02702.x
• 发表时间: 2012-05
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Susumu Yoshizawa;Akira Kawanabe;Hiroyasu Ito;H. Kandori;K. Kogure

Halioglobus japonicus gen. nov., sp. nov., and Halioglobus pacificus sp. nov., within the class Gammaproteobacteria, isolated from seawater

日本盐球菌 (Halioglobus japonicus)

• 发表时间: 2012
• 期刊: International Journal of Systematics and Evolutionary Microbiology(Doi: 10.1099/ijs.0.031443-0)
• 作者: Park;Sanghwa;Susumu Yoshizawa;Kentaro Inomata;Kazuhiro Kogure and Akira Yokota
• 通讯作者: Kazuhiro Kogure and Akira Yokota

Evidence for particle-induced horizontal gene transfer and serial transduction between bacteria

• DOI: 10.1111/j.1574-6941.2011.01077.x
• 发表时间: 2011-06-01
• 期刊: FEMS MICROBIOLOGY ECOLOGY
• 影响因子: 4.2
• 作者: Chiura, Hiroshi Xavier;Kogure, Kazuhiro;Velimirov, Branko
• 通讯作者: Velimirov, Branko