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Physiological roles of lymphatic endothelial cells on lymph formation and transport

淋巴管内皮细胞对淋巴液形成和运输的生理作用

基本信息

批准号：
08457009
负责人：
OHHASHI Toshio
金额：
$ 4.86万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

ACTIVATED MACROPHAGE-MEDIATED ENDOGENOUS PROSTAGLANDIN AND NITRIC OXIDE-DEPENDENT RELAXATION OF LYMPHATIC SMOOTH MUSCLESThe effects of macrophages activated by bacterial lipopolysaccharide (LPS) on the mechanical activity of lymph vessels with or without the endothelium were investigated using conventional bioassay preparations. The supematant of the macrophagtes suppressed significantly the basal tone of the lymphatic bioassay rings precontracted by U46619. The macrophage-induced vasodilation of the lymph vessels was significantly reduced by 12h preincubation of the macrophages with L-NAME,indomethacin, dexamethasone, or cycloheximide. Simultaneous preincubation of L-NAME and indomethacin caused a synergistic reduction of the macrophages-induced vasodilation of the lymphatic bioassay rings. These findings suggest that macrophages activated by bacterial LPS produce a marked relaxation of lymphatic smooth muscles through the co-release of nitric oxide and vasodilative prostaglandins.BAS … More ICPFIBROBLAST GROWTH FACTOR-MEDIATED LYMPHANGIOGENESIS OF LYMPHATIC ENDOTHELIAL CELLS ISOLATED FROM DOG THORACIC DUCTS : EFFECTS OF HEPARINWe have attempted to evaluate whether, similar to angiogenesis of blood vessels, cultures of lymphatic endothelial cells (LEC) isolated from dog thoracic ducts have an ability to induce lymphangiogenesis in response to basic fibroblast growth factor (bFGF) and then to examine effects of heparin on the bFGF-mediated morphogenesis. Effects of bFGF and/or heparin on proliferation and migration of the LEC were also evaluated by changing the number of the subconfluent cells and by wound migration assay, respectively. The effects of the agents on invasion and tube formation of the LEC into a three-dimensional collagen gel and on collagen gel-induced tube formation of the LEC were also investigated by a phase-contrast microscope and an electron microscope. The bFGF caused a significant induction of proliferation and migration of the LEC,the induction of which was augmented dose-dependently by an additional treatment with heparin ranging from 1 to 100 mug/ml. The bFGF produced invasion and tube formation of the LEC into a three-dimensional collagen gel. The bFGF also facilitated to form capillary-like tubes of the LEC between two layrs of colalgen gels. Heparin accelerated the both processes of the bFGF-mediated lymphangiogenesis of the LEC.These findings suggest that the cultured LEC isolated from dog thoracic ducts have an ability to form lymphatic capillary-like tubes in response to bFGF and that heparin accelerates does-dependently the process of the bFGF-mediated neovascularization of lymph vessels. Less

激活的巨噬细胞介导的内源性前列腺素和一氧化氮依赖的淋巴平滑肌松弛用常规生物测定方法观察细菌脂多糖(LPS)激活的巨噬细胞对有或无内皮淋巴管机械活性的影响。巨噬细胞上清液显著抑制U46619预先收缩的淋巴管生物测定环的基础张力。巨噬细胞与L-NAME、消炎痛、地塞米松或放线菌酮共同孵育12h后，巨噬细胞诱导的淋巴管扩张作用明显减弱。同时预先孵育L-NAME和消炎痛可协同降低巨噬细胞诱导的淋巴管生物测定环的血管扩张。这些发现表明，细菌内毒素激活的巨噬细胞通过共同释放一氧化氮和扩张血管的前列腺素而产生显著的淋巴平滑肌松弛。BAS…更多ICPFIBROBLAST生长因子介导的犬胸导管淋巴管内皮细胞的淋巴管生成：HEPARIN我们试图评估从狗胸导管分离的淋巴管内皮细胞(LEC)培养是否具有诱导碱性成纤维细胞生长因子(BFGF)反应的淋巴管生成的能力，然后检测肝素对bFGF介导的形态生成的影响。通过改变亚融合细胞的数量和伤口移行实验，观察bFGF和/或肝素对LEC增殖和迁移的影响。通过相差显微镜和电子显微镜观察药物对晶状体上皮细胞向三维胶原凝胶的侵袭和成管以及对胶原凝胶诱导的晶状体上皮细胞成管的影响。碱性成纤维细胞生长因子可显著诱导晶状体上皮细胞的增殖和迁移，在1~100µg/ml的肝素浓度范围内，这种诱导作用呈剂量依赖性增加，并使其侵袭和形成三维胶原凝胶。碱性成纤维细胞生长因子还有助于在两层胶原凝胶之间形成毛细管状的LEC。肝素可促进bFGF介导的淋巴管生成的两个过程。这些结果表明，培养的犬胸导管LEC具有形成毛细淋巴管样管的能力，且肝素可依赖地加速bFGF介导的淋巴管新生血管的形成。较少