Molecular and physiological approach for addressing the mechanisms of micrometastasis of carcinoma cells within the sentinel lymph node

我们认为前哨淋巴结(SLN)经常受到高淋巴流率的影响。根据它们的生理和生物力学特性，我们推测SLN含有淋巴管内皮细胞(LEC)，这些淋巴管内皮细胞持续承受着高水平的剪应力。为了验证这一假设，我们研究了剪应力刺激对从乳腺癌SLN附近淋巴管分离的人LEC上黏附分子表达和对人LEC释放ATP的影响。综上所述，剪应力刺激通过激活细胞表面F1/FO-ATP合成酶诱导人晶状体上皮细胞ICAM-1的表达，这可能参与了SLN内转移前环境的形成。

We have considered the sentinel lymph node (SLN) to be constantly subjected to a high lymph flow rate. According to their physiological and biomechanical characteristics, thus we hypothesized that SLN contains lymphatic endothelial cells (LEC) that are constantly loaded with high levels of shear stress. To test this hypothesis, we investigated the effects of shear stress stimulation on the expression of adhesion molecules on human LEC isolated from the lymph vessels nearest the SLN of breast cancers, and on the release of ATP from human LEC. In conclusion, shear stress stimulation induces ICAM-1 expression on human LEC by activity cell surface F1/FO ATP synthase, which might contribute to the development of premetastatic environment within SLN.