Molecular and physiological approach for addressing the mechanisms of micrometastasis of carcinoma cells within the sentinel lymph node

我们认为前哨淋巴结（SLN）经常受到高淋巴流速的影响。根据SLN的生理和生物力学特征，我们假设SLN中含有淋巴内皮细胞（LEC），它们持续承受高水平的剪切应力。为了验证这一假设，我们研究了剪切应力刺激对从离乳腺癌SLN最近的淋巴管中分离的人LEC粘附分子表达的影响，以及对人LEC释放ATP的影响。综上所述，剪切应力刺激通过激活细胞表面F1/FO ATP合酶诱导人LEC上ICAM-1的表达，这可能与SLN内转移前环境的形成有关。

We have considered the sentinel lymph node (SLN) to be constantly subjected to a high lymph flow rate. According to their physiological and biomechanical characteristics, thus we hypothesized that SLN contains lymphatic endothelial cells (LEC) that are constantly loaded with high levels of shear stress. To test this hypothesis, we investigated the effects of shear stress stimulation on the expression of adhesion molecules on human LEC isolated from the lymph vessels nearest the SLN of breast cancers, and on the release of ATP from human LEC. In conclusion, shear stress stimulation induces ICAM-1 expression on human LEC by activity cell surface F1/FO ATP synthase, which might contribute to the development of premetastatic environment within SLN.