Effects of occlusal force on homeostasis of microcirculation in periodontal ligament.

1.我们已经开发了一种在体微血管床实验模型，该模型以0.5至1秒的间隔对毛细血管后小静脉进行轻度间歇性机械按压。这种刺激被认为是牙周膜在咀嚼过程中受到的压迫.我们已经观察到增加的血管通透性反应和白细胞运动的变化，在毛细血管后微静脉的机械压缩期间和之后，使用活体显微镜，并提出了这些结果在第29届科学大会的韩国正畸医师协会。我们也观察了年龄对微循环对这种机械刺激的反应的影响，并在第55届日本正畸学会年会上发表了结果。我们还用免疫组化技术进行了另一项实验，以阐明引起这些反应的因素。结果，我们能够检测到P-选择素，一种粘附分子，在受压区周围的内皮细胞上的出现。我们还在第22届日本微循环学会年会上报告了这些结果。

1. We have developed an in vivo microvascular bed experimental model which produces the stimulation on a post capillary venule with mild intermittent mechanical compressions of 0.5 to 1-second intervals. This type of stimulation is assumed to be the compressions of periodontal ligament during mastication.2. We have observed the increased vascular permeability reaction and the changes of leukocyte movement in a post-capillary venule during and after mechanical compression using an intra-vital microscopy, and presented these results at 29th Scientific Congress of Korean Association of Orthodontists. We have also observed the effects of aging on the responses of microcirculation to such mechanical stimulation, and presented the results at 55th Annual Meeting of Japan Orthodontic Society.3. We have made another experiment to clarify the factors inducing these responses by means of immunohistochemical technique. As a result, we were able to detect the appearance of P-selectin, a kind of adhesion molecules, on the endothelial cells around the compressed area. We have also reported these results at 22th Annual Meeting of Japanese Society for Microcirculation.