Rdgulation Mechanisms of Intracellular Membrane Traffic

细胞内膜运输的调节机制

• 批准号: 08458190
• 负责人: NAKANOM Akihiko
• 金额: $ 3.97万
• 依托单位: The Institute of Physical and Chemical Research (RIKEN) (1997-1998)The University of Tokyo (1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458190/
• 关键词: protein secretion; ER-to-Golgi transport; SARI; SEC12; RERI; RER2; HRR25; yeast(S. cerevisiae); 小胞体-ゴルジ体間輸送; RER; cis-プレニルトランスフェラーゼ; 酵母(s.cerevisiae); RST; COPI; COPII; 酵母(S.cerevisiae); 小胞体(ER); ゴルジ体; 小胞輸送

1. The role of Sar1p in vesicle formation from the endoplasmic reticulum (ER) was investigated in tenus of the regulation of its GTPase cycle. EKS1/HRD3 and SED4 were identified as multicopy suppressors of sar1 ts mutants and suggested to be good candidates of GTPase regulators.2. The regulation of Sec12p, which is guanine-nucleotide exchange factor of Sar1p, was also studied genetically. A loss-of-function type mutation of HRR25, encoding a yeast casein kinase I, suppressed sec12 ts, suggesting that Hrr25p negatively regulates vesicle budding.3. The functions of RERI and RER2, genes involved in correct ER localization of Sec 12p, were extensively studied. RERI was shown to encode a Golgi membrane protein, which was essential for retrieval of not only Sec12p but also Sec7lp and Sec63p from the Golgi to the ER. Their transmembrane domains contained Rer1p-dependent retrieval signal. On the other hand. RER2 was demonstrated to code for cis-prenyltransferase, a key enzyme of dolichol synthesis. In addition to the role in protein glycosylation, novel physiological roles of dolichol were suggested from the phenotypes of rer2.

1。在其GTPase循环的调节中，研究了SAR1P在内质网（ER）中的囊泡形成中的作用。 EKS1/HRD3和SED4被确定为SAR1 TS突变体的多拷贝抑制剂，并建议是GTPase调节剂的良好候选者。2。还对SAR1P的鸟嘌呤核苷酸交换因子的Sec12p的调节也进行了遗传研究。 HRR25的功能丧失型突变，编码酵母酪蛋白激酶I，抑制了SEC12 TS，这表明HRR25P负面调节了囊泡的芽。3。广泛研究了RERI和RER2的功能，即SEC 12p的正确定位的基因。 RERI被证明可以编码高尔基膜蛋白，这不仅是检索Sec12p，还可以从Golgi到ER检索Sec7lp和Sec63p。它们的跨膜结构域包含RER1P依赖性检索信号。另一方面。 RER2被证明是CIS-丙基转移酶的代码，这是Dolichol合成的关键酶。除了在蛋白质糖基化中的作用外，还提出了从RER2的表型中提出多利琴的新生理作用。

项目成果

Takashi Ueda, et al.: "AtGD12, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor"Gene. 206. 137-143 (1998)

Takashi Ueda 等人：“AtGD12，编码 Rab GDP 解离抑制剂的新型拟南芥基因”基因。

Miyuki Sato, et al.: "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in the dolichol synthesis"Mol Cell. Biol.. 19. 471-483 (1999)

Miyuki Sato 等人：“通过内质网蛋白定位突变鉴定的酵母 RER2 基因编码顺式异戊二烯基转移酶，这是多醇合成中的关键酶”Mol Cell。

Yumiko Saito, et al.: "Identification of SEC12, SED4, truncated SEC16 and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase"J. Biochem.. 125. 130-137 (1999)

Yumiko Saito 等人：“鉴定 SEC12、SED4、截短的 SEC16 和 EKS1/HRD3 作为 Sar1 GTPase ts 突变体的多拷贝抑制子”J.

Ueda,T.: "Arabdopsis gene isolated by a novel method for detecting genetic interation in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase" Plant Cell. 8. 2079-2091 (1996)

Ueda,T.：“通过一种检测酵母遗传相互作用的新方法分离出的拟南芥基因编码 Ara4 GTP 酶的 GDP 解离抑制剂”Plant Cell。

Takeuchi, M.: "Isolation of a tabacco cDNA encoding Sar1 GTPacl and analysis of its domirant mutations in vesicnlar traffic using a yeast complementation system" Plant Cell Physiol.39. 590-599 (1998)

Takeuchi, M.：“使用酵母互补系统分离编码 Sar1 GTPacl 的烟草 cDNA 并分析其在囊泡运输中的显性突变”Plant Cell Physiol.39。