Rdgulation Mechanisms of Intracellular Membrane Traffic

细胞内膜运输的调节机制

• 批准号: 08458190
• 负责人: NAKANOM Akihiko
• 金额: $ 3.97万
• 依托单位: The Institute of Physical and Chemical Research (RIKEN) (1997-1998)The University of Tokyo (1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458190/
• 关键词: protein secretion; ER-to-Golgi transport; SARI; SEC12; RERI; RER2; HRR25; yeast(S. cerevisiae); 小胞体-ゴルジ体間輸送; RER; cis-プレニルトランスフェラーゼ; 酵母(s.cerevisiae); RST; COPI; COPII; 酵母(S.cerevisiae); 小胞体(ER); ゴルジ体; 小胞輸送

1. The role of Sar1p in vesicle formation from the endoplasmic reticulum (ER) was investigated in tenus of the regulation of its GTPase cycle. EKS1/HRD3 and SED4 were identified as multicopy suppressors of sar1 ts mutants and suggested to be good candidates of GTPase regulators.2. The regulation of Sec12p, which is guanine-nucleotide exchange factor of Sar1p, was also studied genetically. A loss-of-function type mutation of HRR25, encoding a yeast casein kinase I, suppressed sec12 ts, suggesting that Hrr25p negatively regulates vesicle budding.3. The functions of RERI and RER2, genes involved in correct ER localization of Sec 12p, were extensively studied. RERI was shown to encode a Golgi membrane protein, which was essential for retrieval of not only Sec12p but also Sec7lp and Sec63p from the Golgi to the ER. Their transmembrane domains contained Rer1p-dependent retrieval signal. On the other hand. RER2 was demonstrated to code for cis-prenyltransferase, a key enzyme of dolichol synthesis. In addition to the role in protein glycosylation, novel physiological roles of dolichol were suggested from the phenotypes of rer2.

1.在内质网（ER）囊泡形成中Sar1p的作用在其GTP酶循环调节方面进行了研究。 EKS1/HRD3和SED4被鉴定为sar1 ts突变体的多拷贝抑制因子，并被认为是GTPase调节因子的良好候选者。2． Sec12p（Sar1p 的鸟嘌呤核苷酸交换因子）的调节也进行了遗传学研究。编码酵母酪蛋白激酶I的HRR25的功能缺失型突变抑制了sec12 ts，表明Hrr25p负向调节囊泡出芽。3． RERI 和 RER2（参与 Sec 12p 内质网正确定位的基因）的功能已被广泛研究。 RERI 被证明编码高尔基体膜蛋白，这对于从高尔基体到内质网检索 Sec12p 以及 Sec7lp 和 Sec63p 都至关重要。它们的跨膜结构域包含 Rer1p 依赖性检索信号。另一方面。 RER2 被证明编码顺式异戊二烯基转移酶，这是多醇合成的关键酶。除了在蛋白质糖基化中的作用外，rer2 的表型还表明了 dolichol 的新生理作用。

项目成果

Takashi Ueda, et al.: "AtGD12, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor"Gene. 206. 137-143 (1998)

Takashi Ueda 等人：“AtGD12，编码 Rab GDP 解离抑制剂的新型拟南芥基因”基因。

Miyuki Sato, et al.: "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in the dolichol synthesis"Mol Cell. Biol.. 19. 471-483 (1999)

Miyuki Sato 等人：“通过内质网蛋白定位突变鉴定的酵母 RER2 基因编码顺式异戊二烯基转移酶，这是多醇合成中的关键酶”Mol Cell。

Yumiko Saito, et al.: "Identification of SEC12, SED4, truncated SEC16 and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase"J. Biochem.. 125. 130-137 (1999)

Yumiko Saito 等人：“鉴定 SEC12、SED4、截短的 SEC16 和 EKS1/HRD3 作为 Sar1 GTPase ts 突变体的多拷贝抑制子”J.

Takeuchi, M.: "Isolation of a tabacco cDNA encoding Sar1 GTPacl and analysis of its domirant mutations in vesicnlar traffic using a yeast complementation system" Plant Cell Physiol.39. 590-599 (1998)

Takeuchi, M.：“使用酵母互补系统分离编码 Sar1 GTPacl 的烟草 cDNA 并分析其在囊泡运输中的显性突变”Plant Cell Physiol.39。

Sato,K.: "Rerlp as common machinery for the endoplasmic reticulum localization of membrane proteins" Proc.Natl.Acad.Sci.U.S.A.94. 9693-9698 (1997)

Sato,K.：“Rerlp 作为膜蛋白内质网定位的常见机制”Proc.Natl.Acad.Sci.U.S.A.94。