Evolutionary adaptations of the microtubule cytoskeleton during cell division in the land plant lineage

陆地植物谱系细胞分裂过程中微管细胞骨架的进化适应

• 批准号: 528023844
• 负责人: Professorin Dr. Katharina Bürstenbinder
• 金额: --
• 依托单位: Arbeitsgruppe Zellbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/528023844?language=en
• 关键词: Evolutionary; adaptations; microtubule; cytoskeleton; during

The colonization of terrestrial habitats by ancestral streptophyte algae approximately 450 Mya ago, followed by the rise of land plants relied on the evolution of novel microtubule cytoskeletal arrays and was accompanied by the diversification and emergence of plant-specific microtubule-associated proteins (MAPs) to adjust cellular processes to novel conditions and interactions. One of the key innovations in plant cytoskeleton organization is the emergence of the preprophase band (PPB)/phragmoplast system. This system facilitates a switch in the mode of cell division from a cleavage-based mechanisms to an inside-out mechanism, in which new cell walls are inserted at the cell center. Functional studies in Arabidopsis thaliana identified networks of eukaryotic and plant-specific MAPs that control the formation of the PPB and phragmoplast. The origin, architecture and dynamics of these complexes, however, are still unknown. Plant-specific IQ67 DOMAIN (IQD) proteins emerged as key regulators of PPB formation and division plane control in Arabidopsis thaliana with proposed functions as hubs in microtubule-associated PPI networks. IQDs share scaffold-like properties, physically interact with known regulators of plant cell division, and are implicated in auxin-dependent control of division plane positioning. Our preliminary data indicate emergence of IQDs early during land plant evolution. We hypothesize that plant-specific IQD scaffolds facilitated the rewiring and acquisition of PPIs at the cytoskeleton to support neo-functionalization and functional specialization during plant cell division and growth regulation. In this project, we aim to identify key principles that govern the adaptations of the microtubule cytoskeleton by investigating the evolution of IQD-assembled PPI networks and their function in cell division. We will approach these aims by functional analyses of IQD functions in the liverwort Marchantia polymorpha and by comparative analyses of microtubule-binding and PPI specificities of IQDs from streptophyte algae, bryophytes, and seed plants.

大约450万年前，原始链藻对陆地栖息地的殖民，随后陆地植物的崛起依赖于新型微管细胞骨架阵列的进化，并伴随着植物特有的微管相关蛋白(MAP)的多样化和出现，以调整细胞过程以适应新的条件和相互作用。植物细胞骨架组织的关键创新之一是出现了前期带(PPB)/膜质系统。该系统有助于在细胞分裂模式中从基于分裂的机制切换到内向外机制，其中在细胞中心插入新的细胞壁。在拟南芥中的功能研究确定了控制PPB和膜质形成的真核和植物特异图谱的网络。然而，这些复合体的起源、结构和动力学仍然未知。植物特异的IQ67结构域(IQD)蛋白是拟南芥PPB形成和分裂平面控制的关键调控蛋白，被认为是微管相关PPI网络中的枢纽。IQD具有支架样的性质，在物理上与已知的植物细胞分裂调节因子相互作用，并参与依赖生长素的分裂平面定位控制。我们的初步数据表明，在陆地植物进化的早期就出现了IQD。我们推测，植物特有的IQD支架促进了细胞骨架上PPI的重新布线和获取，以支持植物细胞分裂和生长调节过程中的新功能化和功能特化。在这个项目中，我们的目标是通过研究IQD组装的PPI网络的进化及其在细胞分裂中的功能来确定控制微管细胞骨架适应的关键原理。我们将通过对地钱中IQD功能的功能分析以及链藻、苔藓和种子植物IQD的微管结合和PPI特异性的比较分析来实现这些目标。