Development of Artificial haching in the wild silkmoth, Antheraea yamamai

本课题组研制了天蚕咪唑类化合物人工孵化的原始体。本课题对咪唑类化合物人工孵化及其质量法进行了研究，取得了以下重要成果：1)对多种咪唑类化合物进行了滞留分解和孵化率的筛选。因此，KK-62能更有效地保持较高的孵化率。免疫组织化学染色显示滞育前期幼虫的肠道神经支配和内分泌细胞内有大量的多肽类物质。从滞育前期幼虫的微粒体中分离到约60 kDa的KK-42结合蛋白。2)合成了1-{5-(4-乙基苯氧基)戊基}-咪唑。3)建立了KK-62的质量合成方法。4)用餐具漂白剂和次氯酸钠溶液去除卵壳的简单方法对咪唑类化合物大规模人工孵化非常有效，这一结果将为野生天蚕蛾昆虫技术的发展提供有益的参考。

The primitive artifical hatching with imidazole compound of Antheraea yamamai have been developed by the head investigator. In this project, improved artificial hatching with imidazole compound and it's mass method were examined and the following important results were obtained.1) Many imidazole compounds were screened for diapsue brekdown and hatchability. As a result, KK-62 was more effective to keep higher hatchability. Many peptide-like materials were immunohistochemically observed in the gut innervation and endocrine cells of diapausing pre-larvae. KK-42-binding protein (about 60 kDa) was also isolated from microsome fraction of diapausing pre-larvae.2) As a new and effective compund for diapause breakdown, 1- {5- (4-Ethylphenoxy) pentyl} -imidazole was synthesized. 3) A mass method for the synthesis of KK-62 was achieved. 4) A simple method to remove egg shell with tableware bleach and sodium hypochlorite solution was very effective for a mass artificial hatching with imidazole compound.These results will be very usefule for the development of insect technology used in the wild silkmoth Antheraea yamamai.