Developmental use of novel-bioactive agents identified from Bombyx mori and wild silkmoths
从家蚕和野生蚕中鉴定出的新型生物活性剂的开发利用
基本信息
- 批准号:12356002
- 负责人:
- 金额:$ 27.17万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Cytotoxic activity against rat hepatoma cells (dRLh 84 cell lines) was found in the colleterial glands of the wild wilkmoth Antheraea yamamai This chemical structure was determined as a novel dimmer of catecholacetic acid. We have been performing the synthesis method. DILRG-NH_2has been isolated from diapausing pharate first instar larvae A. yamamai. The amidated pentapeptide and its truncated version ILRG-NH_2, not LRG-NH_2 and the non-amidated pentapeptide, suppressed proliferation of the rat hepatoma cells. This suppression may be due to a cell cycle arrest rather than to an apoptotic/necrotic activity. A novel prenylated flavanone was isolated from ethyl acetate extracts of the hard-wood cutting root of Kinuyutaka, a culture of Morus alba. This prenylated flavanone exhibited cytotoxic activity against rat hepatoma cells. These results indicate that the isolated agents are any candidate for therapeutic use. The subesophageal body of the silkworm, Bombyx mori has been known for a lon … More g time, but its function has not been clarified. We demonstrated that this organ produces lysozyme, a tissue-specific chymotrypsin inhibitor (SCI-SB) and a P27K ortholog of a glycoprotein known fro another moth. As a result, the nature and expression patterns of identified proteins indicate that the organ is an immunity-associated organ. A series of 1-substituted and 1,5-disubstituted imidazoles have been shown to terminate diapause in pharate first instar larvae of A. yamamai and the gypsy moth, Lymantria dispar. To understand the mode of action for the imizadole compounds, we analyzed imidazole compound (KK-42)-binding proteins by affinity chromatography using a synthetic resin-coupled KK-42 analog. Two binding proteins (KK-42BPs) of 40 and 45kDa were isolated in soluble fractions prepared diapausing individuals. The N-terminal amino acid sequence from the first 20 residues was determined for the 45 kDa protein. This result is the first finding of a protein associated with artificial hatching in insects. In addition, we were successful in developing of makeup water of fibroin powder from A. yamamai and propose that a new technology used insect sound is better than the artificial stimulus when directed to both the right and left cerebral hemisphere in EMDR treatment for PTSD. Less
在野蛾腋窝腺中发现了对大鼠肝癌细胞(dRLh 84细胞系)的细胞毒活性,这种化学结构被确定为一种新的儿茶酚乙酸二聚体。我们一直在使用合成方法。dilrg - nh_2已从山蝇滞育一龄幼虫中分离得到。经修饰的五肽及其截断型ILRG-NH_2抑制了大鼠肝癌细胞的增殖,而非LRG-NH_2和未修饰的五肽。这种抑制可能是由于细胞周期阻滞而不是由于凋亡/坏死活性。从桑树硬木切根的乙酸乙酯提取物中分离到一种新型的烯丙基化黄酮。这种烯丙基黄酮对大鼠肝癌细胞具有细胞毒活性。这些结果表明,分离的药物是治疗用途的任何候选药物。家蚕(Bombyx mori)的食道下体早已为人所知,但其功能尚不清楚。我们证明这个器官产生溶菌酶,一种组织特异性凝乳胰蛋白酶抑制剂(SCI-SB)和另一种已知糖蛋白的P27K同源物。因此,鉴定的蛋白质的性质和表达模式表明该器官是免疫相关器官。一系列的1-取代咪唑和1,5-二取代咪唑已被证明可以终止A. yamamai和Lymantria dispar舞毒蛾的一龄幼虫的滞育。为了了解咪唑化合物的作用模式,我们使用合成树脂偶联KK-42类似物,通过亲和层析分析了咪唑化合物(KK-42)结合蛋白。从滞育个体的可溶性组分中分离到两个结合蛋白(KK-42BPs),分别为40和45kDa。测定了45 kDa蛋白的前20个残基的n端氨基酸序列。这一结果是首次在昆虫中发现与人工孵化有关的蛋白质。此外,我们成功开发了山麻丝素粉化妆水,并提出了一种新的技术,利用昆虫声音对左右脑半球进行EMDR治疗创伤后应激障碍的效果优于人工刺激。少
项目成果
期刊论文数量(34)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Growth suppression of rat hepatoma cells by a pentapeptide from Antheraera Yamamai.
Antheraera Yamamai 的五肽抑制大鼠肝癌细胞的生长。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Yang P.;Abe S.;Zhao Y.-P.;An;Y.;Suzuki;K.
- 通讯作者:K.
Growth suppression of rat hepatoma cells by a pentapeptide from Antheraea yamamai.
Antheraea yamamai 的五肽抑制大鼠肝癌细胞的生长。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Yang;P.;Abe;S.;Zhao;Y.-P.;An;Y.;Suzuki;K.
- 通讯作者:K.
Yago M., Horaguchi H., Suzuki K., Abe S., Hashimoto S.: "Cytotoxic activity in the colleterial grands of Antheraea yamamai against rat hepatoma cells"International Journal of Wild Silkmoth & Silk. 7. 53-58 (2002)
Yago M.、Horaguchi H.、Suzuki K.、Abe S.、Hashimoto S.:“Antheraea yamamai 的群落对大鼠肝癌细胞的细胞毒性活性”国际野蚕杂志
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
矢口政志, 永田統之 他3名: "桑根からのラット肝ガン細胞増殖抑制物質の探索"東北蚕糸・昆虫利用研究報告. 26. 20 (2001)
Masashi Yaguchi、Noriyuki Nagata 等 3 人:“从桑根中寻找大鼠肝癌细胞生长抑制物质”东北丝绸和昆虫利用研究报告 26. 20 (2001)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
瓜田章二, 三田村 敏正 他3名: "ウスタビ繭層色素の構造解析"東北蚕糸・昆虫利用研究報告. 26. 17 (2001)
Shoji Urita、Toshimasa Mitamura 等 3 人:“乌斯塔维茧层中色素的结构分析”东北丝绸和昆虫利用研究报告 26. 17 (2001)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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SUZUKI Koichi其他文献
SUZUKI Koichi的其他文献
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{{ truncateString('SUZUKI Koichi', 18)}}的其他基金
Identification of molecules involved in genomic damage and their blood monitoring
鉴定参与基因组损伤的分子及其血液监测
- 批准号:
16K10514 - 财政年份:2016
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effects of innate immune activation induced by infection or tissue damage on the development of thyroid autoimmunity
感染或组织损伤诱导的先天免疫激活对甲状腺自身免疫发展的影响
- 批准号:
24591375 - 财政年份:2012
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
On mechanism of microbubble emission boiling and the application for high heat flux cooling technology
微泡发射沸腾机理及高热流冷却技术应用
- 批准号:
23560246 - 财政年份:2011
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Innate immune activation and thyroid autoimmunity
先天免疫激活和甲状腺自身免疫
- 批准号:
21591187 - 财政年份:2009
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Predisposition of cancer development by global analysis of DNA methylation alterations
通过 DNA 甲基化改变的整体分析来了解癌症发展的倾向
- 批准号:
21591710 - 财政年份:2009
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
DNA methylation alterations and their relationship to genomic instability in gastrointestinal cancer
DNA甲基化改变及其与胃肠道癌症基因组不稳定性的关系
- 批准号:
17591387 - 财政年份:2005
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Changes in gene expression profile and development of autoimmunity in the thyroid following infection.
感染后甲状腺基因表达谱的变化和自身免疫的发展。
- 批准号:
15390296 - 财政年份:2003
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
AN INVESTIGATION OF MICROBUBBLE EMISSION BOILING AND APPLICATION TO ULTRA-HIGH HEAT FLUX COOLING TECHNOLOGY FOR HIGH POWERED ELECTRONIC DEVICES
微气泡发射沸腾及其在大功率电子器件超高热流冷却技术中的应用
- 批准号:
14550200 - 财政年份:2002
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of Activation Mechanism of Calpain on the Basis of its Tertiary Structure
基于钙蛋白酶三级结构的激活机制分析
- 批准号:
12308032 - 财政年份:2000
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Mutation detection of cardiac ion channel genes in sudden death cases
猝死病例心脏离子通道基因突变检测
- 批准号:
11470121 - 财政年份:1999
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
相似海外基金
Development of Artificial haching in the wild silkmoth, Antheraea yamamai
野生蚕蛾 Antheraea yamamai 人工孵化技术的发展
- 批准号:
08556010 - 财政年份:1996
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Structure and function of hormones controlling diapause of pharate first-instar larvae in the wild silkmoth, Antheraea yamamai.
控制野生蚕蛾 Antheraea yamamai 甲虫一龄幼虫滞育的激素的结构和功能。
- 批准号:
05454063 - 财政年份:1993
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Studies on Developmental Biology of Cultured Antheraea yamamai Embryo
培养天蚕胚胎的发育生物学研究
- 批准号:
04454064 - 财政年份:1992
- 资助金额:
$ 27.17万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)