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Developmental use of novel-bioactive agents identified from Bombyx mori and wild silkmoths

从家蚕和野生蚕中鉴定出的新型生物活性剂的开发利用

基本信息

批准号：
12356002
负责人：
SUZUKI Koichi
金额：
$ 27.17万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

项目摘要

Cytotoxic activity against rat hepatoma cells (dRLh 84 cell lines) was found in the colleterial glands of the wild wilkmoth Antheraea yamamai This chemical structure was determined as a novel dimmer of catecholacetic acid. We have been performing the synthesis method. DILRG-NH_2has been isolated from diapausing pharate first instar larvae A. yamamai. The amidated pentapeptide and its truncated version ILRG-NH_2, not LRG-NH_2 and the non-amidated pentapeptide, suppressed proliferation of the rat hepatoma cells. This suppression may be due to a cell cycle arrest rather than to an apoptotic/necrotic activity. A novel prenylated flavanone was isolated from ethyl acetate extracts of the hard-wood cutting root of Kinuyutaka, a culture of Morus alba. This prenylated flavanone exhibited cytotoxic activity against rat hepatoma cells. These results indicate that the isolated agents are any candidate for therapeutic use. The subesophageal body of the silkworm, Bombyx mori has been known for a lon … More g time, but its function has not been clarified. We demonstrated that this organ produces lysozyme, a tissue-specific chymotrypsin inhibitor (SCI-SB) and a P27K ortholog of a glycoprotein known fro another moth. As a result, the nature and expression patterns of identified proteins indicate that the organ is an immunity-associated organ. A series of 1-substituted and 1,5-disubstituted imidazoles have been shown to terminate diapause in pharate first instar larvae of A. yamamai and the gypsy moth, Lymantria dispar. To understand the mode of action for the imizadole compounds, we analyzed imidazole compound (KK-42)-binding proteins by affinity chromatography using a synthetic resin-coupled KK-42 analog. Two binding proteins (KK-42BPs) of 40 and 45kDa were isolated in soluble fractions prepared diapausing individuals. The N-terminal amino acid sequence from the first 20 residues was determined for the 45 kDa protein. This result is the first finding of a protein associated with artificial hatching in insects. In addition, we were successful in developing of makeup water of fibroin powder from A. yamamai and propose that a new technology used insect sound is better than the artificial stimulus when directed to both the right and left cerebral hemisphere in EMDR treatment for PTSD. Less

天蚕（Antheraea yamai）的粘液腺对大鼠肝癌细胞（dRLh 84细胞系）具有细胞毒活性。我们一直在执行合成方法。DILRG-NH_2是从滞育的一龄幼虫A.山井。酰胺化五肽及其截短型ILRG-NH_2抑制大鼠肝癌细胞的增殖，而非酰胺化五肽和LRG-NH_2抑制大鼠肝癌细胞的增殖。这种抑制可能是由于细胞周期停滞，而不是凋亡/坏死活性。从桑属栽培植物Kinuyutaka的硬木扦插根的乙酸乙酯提取物中分离得到一种新的异戊烯基黄烷酮。这种异戊烯化的黄烷酮表现出对大鼠肝癌细胞的细胞毒活性。这些结果表明，分离的试剂是治疗用途的任何候选物。家蚕食道下体的研究由来已久 ...更多信息 g时间，但其功能尚未阐明。我们证明，这个器官产生溶菌酶，组织特异性胰凝乳蛋白酶抑制剂（SCI-SB）和P27 K的直系同源物的糖蛋白已知从另一个蛾。因此，鉴定的蛋白质的性质和表达模式表明该器官是免疫相关器官。一系列1-取代和1，5-二取代咪唑类化合物能解除一龄幼虫的滞育。山麦和舞毒蛾舞毒蛾为了了解咪唑化合物的作用模式，我们使用合成树脂偶联的KK-42类似物通过亲和色谱法分析了咪唑化合物（KK-42）结合蛋白。在滞育个体的可溶性组分中分离到两种40和45 kDa的结合蛋白（KK-42 BPs）。确定45 kDa蛋白的前20个残基的N-末端氨基酸序列。这一结果是首次发现与昆虫人工孵化相关的蛋白质。此外，我们还成功地开发了A. Yamamai提出，在EMDR治疗PTSD中，使用昆虫声音的新技术在针对左右大脑半球时优于人工刺激。少