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Developmental use of novel-bioactive agents identified from Bombyx mori and wild silkmoths

从家蚕和野生蚕中鉴定出的新型生物活性剂的开发利用

基本信息

批准号：
12356002
负责人：
SUZUKI Koichi
金额：
$ 27.17万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

项目摘要

Cytotoxic activity against rat hepatoma cells (dRLh 84 cell lines) was found in the colleterial glands of the wild wilkmoth Antheraea yamamai This chemical structure was determined as a novel dimmer of catecholacetic acid. We have been performing the synthesis method. DILRG-NH_2has been isolated from diapausing pharate first instar larvae A. yamamai. The amidated pentapeptide and its truncated version ILRG-NH_2, not LRG-NH_2 and the non-amidated pentapeptide, suppressed proliferation of the rat hepatoma cells. This suppression may be due to a cell cycle arrest rather than to an apoptotic/necrotic activity. A novel prenylated flavanone was isolated from ethyl acetate extracts of the hard-wood cutting root of Kinuyutaka, a culture of Morus alba. This prenylated flavanone exhibited cytotoxic activity against rat hepatoma cells. These results indicate that the isolated agents are any candidate for therapeutic use. The subesophageal body of the silkworm, Bombyx mori has been known for a lon … More g time, but its function has not been clarified. We demonstrated that this organ produces lysozyme, a tissue-specific chymotrypsin inhibitor (SCI-SB) and a P27K ortholog of a glycoprotein known fro another moth. As a result, the nature and expression patterns of identified proteins indicate that the organ is an immunity-associated organ. A series of 1-substituted and 1,5-disubstituted imidazoles have been shown to terminate diapause in pharate first instar larvae of A. yamamai and the gypsy moth, Lymantria dispar. To understand the mode of action for the imizadole compounds, we analyzed imidazole compound (KK-42)-binding proteins by affinity chromatography using a synthetic resin-coupled KK-42 analog. Two binding proteins (KK-42BPs) of 40 and 45kDa were isolated in soluble fractions prepared diapausing individuals. The N-terminal amino acid sequence from the first 20 residues was determined for the 45 kDa protein. This result is the first finding of a protein associated with artificial hatching in insects. In addition, we were successful in developing of makeup water of fibroin powder from A. yamamai and propose that a new technology used insect sound is better than the artificial stimulus when directed to both the right and left cerebral hemisphere in EMDR treatment for PTSD. Less

在野蛾腋窝腺中发现了对大鼠肝癌细胞（dRLh 84细胞系）的细胞毒活性，这种化学结构被确定为一种新的儿茶酚乙酸二聚体。我们一直在使用合成方法。dilrg - nh_2已从山蝇滞育一龄幼虫中分离得到。经修饰的五肽及其截断型ILRG-NH_2抑制了大鼠肝癌细胞的增殖，而非LRG-NH_2和未修饰的五肽。这种抑制可能是由于细胞周期阻滞而不是由于凋亡/坏死活性。从桑树硬木切根的乙酸乙酯提取物中分离到一种新型的烯丙基化黄酮。这种烯丙基黄酮对大鼠肝癌细胞具有细胞毒活性。这些结果表明，分离的药物是治疗用途的任何候选药物。家蚕（Bombyx mori）的食道下体早已为人所知，但其功能尚不清楚。我们证明这个器官产生溶菌酶，一种组织特异性凝乳胰蛋白酶抑制剂（SCI-SB）和另一种已知糖蛋白的P27K同源物。因此，鉴定的蛋白质的性质和表达模式表明该器官是免疫相关器官。一系列的1-取代咪唑和1,5-二取代咪唑已被证明可以终止A. yamamai和Lymantria dispar舞毒蛾的一龄幼虫的滞育。为了了解咪唑化合物的作用模式，我们使用合成树脂偶联KK-42类似物，通过亲和层析分析了咪唑化合物（KK-42）结合蛋白。从滞育个体的可溶性组分中分离到两个结合蛋白（KK-42BPs），分别为40和45kDa。测定了45 kDa蛋白的前20个残基的n端氨基酸序列。这一结果是首次在昆虫中发现与人工孵化有关的蛋白质。此外，我们成功开发了山麻丝素粉化妆水，并提出了一种新的技术，利用昆虫声音对左右脑半球进行EMDR治疗创伤后应激障碍的效果优于人工刺激。少