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Developmental use of novel-bioactive agents identified from Bombyx mori and wild silkmoths

从家蚕和野生蚕中鉴定出的新型生物活性剂的开发利用

基本信息

批准号：
12356002
负责人：
SUZUKI Koichi
金额：
$ 27.17万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

项目摘要

Cytotoxic activity against rat hepatoma cells (dRLh 84 cell lines) was found in the colleterial glands of the wild wilkmoth Antheraea yamamai This chemical structure was determined as a novel dimmer of catecholacetic acid. We have been performing the synthesis method. DILRG-NH_2has been isolated from diapausing pharate first instar larvae A. yamamai. The amidated pentapeptide and its truncated version ILRG-NH_2, not LRG-NH_2 and the non-amidated pentapeptide, suppressed proliferation of the rat hepatoma cells. This suppression may be due to a cell cycle arrest rather than to an apoptotic/necrotic activity. A novel prenylated flavanone was isolated from ethyl acetate extracts of the hard-wood cutting root of Kinuyutaka, a culture of Morus alba. This prenylated flavanone exhibited cytotoxic activity against rat hepatoma cells. These results indicate that the isolated agents are any candidate for therapeutic use. The subesophageal body of the silkworm, Bombyx mori has been known for a lon … More g time, but its function has not been clarified. We demonstrated that this organ produces lysozyme, a tissue-specific chymotrypsin inhibitor (SCI-SB) and a P27K ortholog of a glycoprotein known fro another moth. As a result, the nature and expression patterns of identified proteins indicate that the organ is an immunity-associated organ. A series of 1-substituted and 1,5-disubstituted imidazoles have been shown to terminate diapause in pharate first instar larvae of A. yamamai and the gypsy moth, Lymantria dispar. To understand the mode of action for the imizadole compounds, we analyzed imidazole compound (KK-42)-binding proteins by affinity chromatography using a synthetic resin-coupled KK-42 analog. Two binding proteins (KK-42BPs) of 40 and 45kDa were isolated in soluble fractions prepared diapausing individuals. The N-terminal amino acid sequence from the first 20 residues was determined for the 45 kDa protein. This result is the first finding of a protein associated with artificial hatching in insects. In addition, we were successful in developing of makeup water of fibroin powder from A. yamamai and propose that a new technology used insect sound is better than the artificial stimulus when directed to both the right and left cerebral hemisphere in EMDR treatment for PTSD. Less

在野生威尔克莫斯 Antheraea yamamai 的结肠腺中发现了针对大鼠肝癌细胞（dRLh 84 细胞系）的细胞毒性活性。该化学结构被确定为儿茶酚乙酸的新型二聚体。我们一直在进行合成方法。 DILRG-NH_2 是从滞育法甲动物一龄幼虫 A. yamamai 中分离出来的。酰胺化五肽及其截短形式ILRG-NH_2，而不是LRG-NH_2和非酰胺化五肽，抑制了大鼠肝癌细胞的增殖。这种抑制可能是由于细胞周期停滞而不是细胞凋亡/坏死活性。从桑树培养物 Kinuyutaka 的硬木切根的乙酸乙酯提取物中分离出一种新型异戊二烯化黄烷酮。这种异戊二烯化黄烷酮对大鼠肝癌细胞表现出细胞毒活性。这些结果表明分离的药剂是治疗用途的任何候选者。家蚕的食管下体早已为人所知，但其功能尚未阐明。我们证明该器官产生溶菌酶、一种组织特异性胰凝乳蛋白酶抑制剂 (SCI-SB) 和另一种蛾类已知糖蛋白的 P27K 直系同源物。因此，所鉴定蛋白质的性质和表达模式表明该器官是免疫相关器官。一系列 1-取代和 1,5-二取代咪唑已被证明可以终止 A. yamamai 和舞毒蛾 (Lymantria dispar) 的法甲动物一龄幼虫的滞育。为了了解咪唑化合物的作用模式，我们使用合成树脂偶联的 KK-42 类似物通过亲和层析分析了咪唑化合物 (KK-42) 结合蛋白。在滞育个体制备的可溶性级分中分离出两种 40 和 45kDa 的结合蛋白 (KK-42BP)。确定 45 kDa 蛋白质的前 20 个残基的 N 端氨基酸序列。这一结果是首次发现与昆虫人工孵化相关的蛋白质。此外，我们成功地开发了来自A.yamamai的蚕丝蛋白粉化妆水，并提出在针对PTSD的EMDR治疗中，当针对左右大脑半球时，使用昆虫声音的新技术比人工刺激更好。较少的