Physiological studies on the physiological and behavioral changes after mating in the moth, Bombyx mori

抑制信息素生产后交配蛾似乎是由一个神经信号，来自腹部和运行的腹神经索，以抑制释放的信息素亲神经肽。本研究表明，神经分泌细胞释放的神经肽是最敏感的机械刺激，其放电活动完全抑制交配开始。因此，在交配期间由物理刺激诱导的机械信号可以触发神经分泌细胞的失活机制。因为与不育的雄性交配不能引起细胞的永久抑制。如果它们不能接收由从可育雄性转移的精子和/或睾丸因子诱导的另一个信号，则可以释放由机械刺激引起的暂时抑制。将从交配雌虫的交配囊中获得的精液人工注射到已接受tacti的雌虫体内， 关于我们 来自不育雄性的较少刺激，发生永久抑制信息素产生，由此表明，在交配的雌性中，性信息素产生的两步失活机制是真实的。从对交配的舞毒蛾的观察中已经推断出类似的两步失活机制。雌蛾在交配后固定在平台上，交配后的雌蛾往往又重新开始产生信息素。雌虫在95%CO_2中麻醉15分钟或乙醚中麻醉1-2分钟后，也可恢复产生信息素。结果表明，在应激条件下，如束缚或麻醉，这种永久性抑制可以被释放，突变株（XU 20）的未交配产卵的处女雌虫具有很低的性信息素含量，但在脑-食管下神经节提取物中具有大量的PBAN活性，由此表明，释放信息素促神经肽的神经分泌细胞甚至在处女雌性中也可能失活。少

Suppression of pheromone production after mating in moths seems to be induced by a neural signal that originates from the abdomen and runs up the ventral nerve cord to inhibit the release of pheromonotropic neuropeptides. The present study demonstrated that the neurosecretory cells releasing the neuropeptides were most sensitive to mechanical stimulation and their firing activities were completely suppressed as soon as mating began. Thus, a mechanical signal induced by physical stimulation during copulation can trigger an inactivation mechanism of the neurosecretory cells. Because mating with a sterile male failed to induce permanent suppression of the cells. a temporal inhibition caused by mechanical stimuli may be released if they cannot receive another signal that may be induced by the sperm and /or testicular factors transferred from a fertile male. When semen obtained from the bursa copulatorix of a mated female were artificially injected into a female which already received tacti … More le stimulation from a sterile male, permanent suppression of pheromone production occurred, thereby indicating that the two-step inactivation mechanism of sex pheromone production is true in a mated female.A similar two-step inactivation mechanism has been inferred from observations of mated gypsy moth.Identification and mode of action of the putative-pheromonostatic male factor in Bombyx mori remain to be determined.Pheromone production often resumed again in mated moths when the females were fixed to a platform after mating. Similar resumption of pheromone production were observed in the females which were anesthetized for 15 min in 95% CO_2 or for 1-2 min in etylether. The results suggest that the permanent suppressioncan be released under some stressful condition, such as restraint or anesthesia.Virgin females of a mutant strain (XU20), which lay eggs without mating, had very low amount of sex pheromone but had large amonunt of PBAN activity in the extract of the brain-suboesophageal ganglion, thereby suggesting that the neurosecretory cells releasing pheromonotropic neuropeptides may be inactivated even in a virgin female. Less