Physiological studies on the physiological and behavioral changes after mating in the moth, Bombyx mori

家蚕蛾交配后生理和行为变化的生理学研究

基本信息

批准号：
08640870
负责人：
ICHIKAWA Toshio
金额：
$ 1.41万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Suppression of pheromone production after mating in moths seems to be induced by a neural signal that originates from the abdomen and runs up the ventral nerve cord to inhibit the release of pheromonotropic neuropeptides. The present study demonstrated that the neurosecretory cells releasing the neuropeptides were most sensitive to mechanical stimulation and their firing activities were completely suppressed as soon as mating began. Thus, a mechanical signal induced by physical stimulation during copulation can trigger an inactivation mechanism of the neurosecretory cells. Because mating with a sterile male failed to induce permanent suppression of the cells. a temporal inhibition caused by mechanical stimuli may be released if they cannot receive another signal that may be induced by the sperm and /or testicular factors transferred from a fertile male. When semen obtained from the bursa copulatorix of a mated female were artificially injected into a female which already received tacti … More le stimulation from a sterile male, permanent suppression of pheromone production occurred, thereby indicating that the two-step inactivation mechanism of sex pheromone production is true in a mated female.A similar two-step inactivation mechanism has been inferred from observations of mated gypsy moth.Identification and mode of action of the putative-pheromonostatic male factor in Bombyx mori remain to be determined.Pheromone production often resumed again in mated moths when the females were fixed to a platform after mating. Similar resumption of pheromone production were observed in the females which were anesthetized for 15 min in 95% CO_2 or for 1-2 min in etylether. The results suggest that the permanent suppressioncan be released under some stressful condition, such as restraint or anesthesia.Virgin females of a mutant strain (XU20), which lay eggs without mating, had very low amount of sex pheromone but had large amonunt of PBAN activity in the extract of the brain-suboesophageal ganglion, thereby suggesting that the neurosecretory cells releasing pheromonotropic neuropeptides may be inactivated even in a virgin female. Less

在飞蛾中交配后的信息素产生的抑制似乎是由源自腹部并延伸通气神经索的神经病学诱导的，以抑制对球质神经肽的释放。本研究表明，释放神经肽的神经分泌细胞对机械刺激最敏感，一旦交配开始，它们的发射活性就会完全抑制。这是由物理刺激在交配过程中引起的机械信号可以触发神经分泌细胞的灭活机制。因为与无菌雄性交配未能诱导对细胞的永久抑制。如果机械刺激引起的暂时抑制作用，如果他们无法收到可能由肥胖男性转移的精子和 /或有证据的因素引起的另一个信号。当从伴侣的Bursa Copulatorix获得的精液被人为地注入了已经接受了触觉的女性中……更多的LE刺激是从无菌男性中产生的，永久性地抑制了信息素的产生，从而表明，两步性的灭活性生产机制是在类似的两种状态中造成的。在Bombyx Mori中，推定的手感男性因素的作用方式仍有待确定。当女性在交配后将女性固定到平台上时，经常在交配的飞蛾中再次恢复了雌酮的产生。在雌性中观察到了类似的信息素产生，这些女性在95％的CO_2中麻醉15分钟或在乙醚中持续1-2分钟。结果表明，永久性抑制可以在某种压力状态下释放，例如约束或麻醉。突变菌株（XU20）的Virgin女性在不交配的情况下产卵的女性（XU20）具有很低的性信息信息素，但在PBAR活动中具有很大的性能，但在脑胃持续的神经伴侣的提取物中具有很大的活性即使在处女中被灭活。较少的