Physiological studies on the physiological and behavioral changes after mating in the moth, Bombyx mori

家蚕蛾交配后生理和行为变化的生理学研究

• 批准号: 08640870
• 负责人: ICHIKAWA Toshio
• 金额: $ 1.41万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640870/
• 关键词: Bombyx mori; sex pheromone; mating; neurosecretory cell; neuropeptide; semen; inactivation; anesthesia.; 昆虫; ペプチドホルモン; 電気的活動; 行動異常; サーカディアンリズム; ストレス

Suppression of pheromone production after mating in moths seems to be induced by a neural signal that originates from the abdomen and runs up the ventral nerve cord to inhibit the release of pheromonotropic neuropeptides. The present study demonstrated that the neurosecretory cells releasing the neuropeptides were most sensitive to mechanical stimulation and their firing activities were completely suppressed as soon as mating began. Thus, a mechanical signal induced by physical stimulation during copulation can trigger an inactivation mechanism of the neurosecretory cells. Because mating with a sterile male failed to induce permanent suppression of the cells. a temporal inhibition caused by mechanical stimuli may be released if they cannot receive another signal that may be induced by the sperm and /or testicular factors transferred from a fertile male. When semen obtained from the bursa copulatorix of a mated female were artificially injected into a female which already received tacti … More le stimulation from a sterile male, permanent suppression of pheromone production occurred, thereby indicating that the two-step inactivation mechanism of sex pheromone production is true in a mated female.A similar two-step inactivation mechanism has been inferred from observations of mated gypsy moth.Identification and mode of action of the putative-pheromonostatic male factor in Bombyx mori remain to be determined.Pheromone production often resumed again in mated moths when the females were fixed to a platform after mating. Similar resumption of pheromone production were observed in the females which were anesthetized for 15 min in 95% CO_2 or for 1-2 min in etylether. The results suggest that the permanent suppressioncan be released under some stressful condition, such as restraint or anesthesia.Virgin females of a mutant strain (XU20), which lay eggs without mating, had very low amount of sex pheromone but had large amonunt of PBAN activity in the extract of the brain-suboesophageal ganglion, thereby suggesting that the neurosecretory cells releasing pheromonotropic neuropeptides may be inactivated even in a virgin female. Less

飞蛾交配后信息素产生的抑制似乎是由来自腹部的神经信号引起的，并沿着腹神经索向上传播，以抑制信息素神经肽的释放。目前的研究表明，释放神经肽的神经分泌细胞对机械刺激最敏感，一旦交配开始，它们的放电活动就被完全抑制。因此，交配过程中物理刺激引起的机械信号可以触发神经分泌细胞的失活机制。因为与不育雄性交配未能引起细胞的永久抑制。如果它们无法接收由可育男性转移的精子和/或睾丸因子诱导的另一个信号，则可能会释放由机械刺激引起的暂时抑制。当将从交配雌性交配囊中获得的精液人工注射到已接受不育雄性触觉刺激的雌性体内时，会发生信息素产生的永久性抑制，从而表明性信息素产生的两步失活机制在交配雌性中是正确的。从交配吉普赛蛾的观察中推断出类似的两步失活机制。 家蚕中假定的信息素抑制雄性因子的作用方式仍有待确定。当雌性在交配后被固定在平台上时，信息素的产生通常会在交配蛾中再次恢复。在95％CO_2中麻醉15分钟或在乙醚中麻醉1-2分钟的雌性中观察到类似的信息素产生恢复。结果表明，在某些应激条件下，例如约束或麻醉，可以释放永久性抑制。突变株（XU20）的处女雌性不交配产卵，其性信息素含量极低，但在脑食管下神经节提取物中具有大量PBAN活性，从而表明神经分泌细胞释放信息素 即使在处女体内，神经肽也可能失活。较少的

项目成果

Ichikawa, Toshio: "Activity patterns of neurosecretory cells releasing pheromonotropic neuropeptides in the moth,Bombyx mori" Proceeding of National Academy of Sciences,USA. (印刷中).

Ichikawa, Toshio：“家蚕中释放信息信息神经肽的神经分泌细胞的活动模式”美国国家科学院院刊（正在出版）。

Ichikawa, T., Shiota, T., Shimizu, I.And Kataoka, H.: "Functional differntiation of neurosecretory cells with imminoreactive daiapause hormone and pheromne biosynthesis activating neuropeptide in the moth, Bombyx mori" Zoological Science. 13. 21-26 (1996)

Ichikawa，T.，Shiota，T.，Shimizu，I.和Kataoka，H.：“神经分泌细胞与免疫反应性停经激素和信息素生物合成激活神经肽在蛾，家蚕中的功能分化”动物学科学。

T.Ichikawa et al.: "Functional differentiation of neurosecretory cells with imminoreactive daiapause hormone and pheromone biosynthesis activating neuropeptide of the silkworm" Zoological Science. 13. 21-26 (1996)

T.Ichikawa 等人：“神经分泌细胞与免疫反应性滞育激素和信息素生物合成激活蚕神经肽的功能分化”《动物学科学》。

I.Shimizu et al.: "Neuroendocrine control of diapause hormone secretion in the silkworm,Bombyx mori" Jouranal of Insect Physiology. 43. 1101-1109 (1997)

I.Shimizu 等人：“家蚕滞育激素分泌的神经内分泌控制”昆虫生理学杂志。

Ichikawa, T.: "Activity patterns of neurosecretory cells releasing pheromonotropic neuropeptides in the moth, Bombyx mori" Proc.Natl.Acad.Sci.USA. (in Press).

Ichikawa, T.：“家蚕蛾中释放信息信息神经肽的神经分泌细胞的活动模式”Proc.Natl.Acad.Sci.USA。