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Physiological study of synchronous firing activity in insect neurosecretory cell systems

昆虫神经分泌细胞系统同步放电活动的生理研究

基本信息

批准号：
11640681
负责人：
ICHIKAWA Toshio
金额：
$ 2.24万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

There are four pairs of neurosecretory cells producing bombyxin, an insulin-related peptide, in the brain of Bombyx mori. Electrical signals of the bombyxin-producing cells were identified and short-and long-term activity patterns of the cells throughout the pupal period were analyzed. Activity pattern of the cells was characterized by periodic discharges of a volley of action potentials. Periodicity of the electrical activity depended on the temperature and mean±SD of periods was 34.7±4.8 min at 26℃. Four cells in a brain hemisphere were functionally heterogeneous : individual cells had specific activity amplitudes and rhythms, thereby suggesting that an ultradian activity rhythm of a cell originates from its intrinsic pacemaker activity. There was no significant diel (circadian) oscillation in the electrical activity. Daily activity patterns had a maximum at an early stage (day-2 or 3) of pupal period. Firing correlations were examined among four pairs of bombyxin-producing cells with an ultradian bursting rhythm. Cross-correlation analyses of spiking activities between pairs of simultaneously active cells usually revealed a significant correlation of firings : 20-50% of spikes of a cell fired within 0.2 s before or after the other cell's spikes fired. The mutual, excitatory communication among neurosecretory cells make the set of cells a weakly coupled multi-oscillator system that may generates a pulsatile release pattern of their hormone that seems to be an efficient temporal pattern for maintaining hormonal actions.

家蚕脑内有4对分泌细胞，分泌胰岛素相关肽--蚕毒素。电信号的家蚕毒素生产细胞进行了鉴定和短期和长期的活动模式的细胞在整个蛹期进行了分析。细胞的活动模式的特征在于动作电位的周期性放电。电活动的周期性取决于温度，在26℃下，周期的平均值±SD为34.7±4.8 min。大脑半球中的四个细胞在功能上是异质的：单个细胞具有特定的活动幅度和节奏，从而表明细胞的超日活动节奏源于其内在的起搏器活动。在电活动中没有明显的昼夜（昼夜）振荡。日活动模式在蛹期的早期（第2或第3天）有最大值。研究了四对具有超日爆发节律的产蚕毒素细胞之间的放电相关性。对同时活跃的细胞之间的尖峰活动的交叉相关分析通常揭示了发射的显着相关性：20-50%的细胞的尖峰在0.2秒内发射之前或之后其他细胞的尖峰发射。神经分泌细胞之间的相互的、兴奋性的通信使细胞的集合成为弱耦合的多振荡器系统，该系统可以产生它们的激素的脉冲式释放模式，这似乎是维持激素作用的有效的时间模式。