Studies on microbial and chemical control of methane production from the rumen

瘤胃甲烷通过教育释放到环境中。最近，甲烷因其对全球变暖的贡献而受到关注。本试验旨在研究饲粮中添加环糊精（CD）包封的溴氯甲烷（BCM）和二氯甲烷（DCM）对牛甲烷和氢气生成、瘤胃发酵和纤维消化的影响。添加1g/lOOkgBW/d的BCM-CD，从添加后第1天起，甲烷排放量显著降低。另一方面，BCM-CD的加入显著增加了氢排放。瘤胃乙酸摩尔比降低，丙酸摩尔比升高。瘤胃总VFA浓度和pH值不变。添加1g/lOOkgBW/d的DCM-CD对甲烷产量和瘤胃发酵无影响。添加BCM的效果。-CD对甲烷产量的影响通过开路呼吸系统测量。BCM-CD的加入使甲烷产量降低了95%。采食量、日粮纤维表观消化率、瘤胃纤毛虫原虫浓度、VFA和氨含量不变。添加BCM-CD可降低瘤胃乙酸的摩尔比，提高瘤胃丙酸的摩尔比。上述结果表明，BCM-CD在不影响瘤胃纤维消化的情况下，对甲烷的产生有明显的抑制作用。

Ruminal methane is released into the environment info by eructation. Recently, methane has received attention due to its contribution to global warming. The aims of this study were to determine the effect of dietary addition of bromochioromethane (BCM) and dichloromethane (DCM) encapsulated in a cyclodextrin (CD) matrix on methane and hydrogen production, rumen fermentation and fiber digestion in cattle.The addition of 1g/lOOkgBW/day BCM-CD decreased methane emission greatly from i day after the start of addition. On the other hand, addition of BCM-CD increased hydrogen emission markedly. Molar proportion of ruminal acetic acid decreased and that of propionic acid increased. Ruminal concentration of total VFA and pH value were unchanged.The addition of 1g/lOOkgBW/day DCM-CD had no effects on methane production and rumen fermentation.Effect of the addition of BCM.-CD on methane production was measured by an open-circuit respiratory system. The addition of BCM-CD decreased methane production by 95%. Feed intake, apparent digestibility of dietary fibre, ruminal concentration of ciliate protozoa, VFA and ammonia were unchanged. Molar proportion of ruminal acetic acid decreased and that of propionic acid increased by the addition of BCM-CD.These results suggest that BCM-CD enable the potent inhibiting effect on methane production without affecting the fiber digestion in the rumen.