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Changes of the cytoskeletal structures in the peritubular myoid cells in the testis and their effects on spermatogenesis

睾丸管周肌样细胞细胞骨架结构的变化及其对精子发生的影响

基本信息

批准号：
08670007
负责人：
MAEKAWA Mamiko
金额：
$ 1.15万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670007/
关键词：
Spermatogenesis Testis Myoid cell Actin filament Cytoskeleton α-アクチニン

项目摘要

1.Postnatal development of the arrangement of actin filaments in myoid cellsOnly circular bundles of actin filaments were seen in the rat myoid cells at 15 days of age, and longitudinal bundles appeared at 30 days. The lattice arrangement of the actinfilaments, whicih was seen in the adult testis, was completed at 40 days when elongated spermatids occurred in the seminiferous epithelium. These data suggest that the myoid cells may mature morphologically before the completion of spermatogenesis.2.Effect of cryptorchidism on actin filaments in myoid cellsThe induction of experimental cryptorchidism results in the disruption of spermatogenesis by high abdominal temperature. Seven days after abdominal confinement, the arrangement of actin filaments in the rat myoid cells showed a remarkable disruption. This result suggests that the arrangement of actin filaments in the myoid cell is important for normal spermatogenesis.3.Distribution of alpha-actinin in myoid cellsOne of the actin-binding proteins, alpha-actinin, was immunolocalized in the rat myoid cells by confocal laser microscopy and immuno-electron microscopy. This protein was distributed as dots in the actin filament bundles of the myoid cells, and appeared to exist in dense bodies of the myoid cells. These results correspond to other reports showing the existence of the alpha-actinin in dense bodies of smooth muscle cells in the chicken gizzard and in stomach muscle layrs.4.Relationship between cytoskeleton and Fyn tyrosine kinase in the mouse testisTo study further on the function of cytoskeletal proteins in the testis, distribution of Fyn tyrosine kinase was investigated. Fyn is a member of the Src family, and Src plays a role in cell growth and differentiation, interacting with cytoskeletal proteins. Immunohistochemical results showed that Fyn protein was localized in Sertoli cells in the mouse testis, and that was colocalized with actin filaments.

1.肌样细胞肌动蛋白丝排列的生后发育15日龄大鼠肌样细胞中仅可见环状的肌动蛋白丝束，30日龄出现纵向的肌动蛋白丝束。40日龄生精上皮细胞中出现细长的精子细胞，使成熟睾丸中的肌动丝形成格子状排列。这些结果表明，在精子发生完成之前，肌样细胞可能在形态上已经成熟。2.隐睾对肌样细胞肌动蛋白丝的影响实验性隐睾的诱导导致了腹腔高温对精子发生的破坏。腹部禁闭后7天，大鼠肌样细胞中肌动蛋白丝的排列显示出显着的破坏。这一结果表明，肌样细胞中肌动蛋白丝的排列对于正常的精子发生很重要。3.肌样细胞中α-辅肌动蛋白的分布肌动蛋白结合蛋白之一α-辅肌动蛋白通过共聚焦激光显微镜和免疫电子显微镜在大鼠肌样细胞中进行免疫定位。该蛋白质呈点状分布于肌样细胞的肌动蛋白丝束中，似乎存在于肌样细胞的致密体中。这些结果与其它报道的鸡砂囊和胃平滑肌层平滑肌细胞致密体中存在α-辅肌动蛋白的结果一致。4.小鼠睾丸细胞骨架蛋白与Fyn酪氨酸激酶的关系为了进一步研究睾丸细胞骨架蛋白的功能，本研究对Fyn酪氨酸激酶的分布进行了研究。Fyn是Src家族的成员，Src在细胞生长和分化中起作用，与细胞骨架蛋白相互作用。免疫组化结果显示，Fyn蛋白定位于小鼠睾丸支持细胞，与肌动蛋白丝共定位。