Characterization of recognition specificity and analysis of variety of recognition proteins of BT toxin.

BT毒素多种识别蛋白的识别特异性表征及分析。

• 批准号: 08660408
• 负责人: SATO Ryoichi
• 金额: $ 1.54万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660408/
• 关键词: Bacillus thuringiensis; delta-endotoxins; δ-内毒素; タンパク質認識; 殺虫性タンパク質; 認識タンパク質

Three BT toxins (delta-endotoxins of Bacillus thuringiensis) were investigated on their bringing targets and binding characters, and results were gained as follows.1. Three BT toxins bound to some proteins which is not seen on the insect cells but used as experimental reagents. Binding-proteins of the three BT toxines was almost the same each other. 2. Affinity of BT toxins and the binding-proteins were about 100 times lowere than that of BT toxins and their receptors. 3. All three BT toxins bound to carbonic anhydorase from bovine red blood cell and RNase A from bovine pancrae. These binding-proteins have amino acid sequences with high homology each other, which form alpha-helicses. 4. It was shown that BT toxin bind to the binding-proteins on the portion which is used to bind to the insect cell receptors. 5. It was suggested that one of three BT toxins utilise aminopeptidase N as a receptor on the silkworm midgut cells.

对三种BT毒素（苏云金杆菌δ-内毒素）的作用靶点和结合特性进行了研究，得到以下结果：1.三种BT毒素与昆虫细胞上没有的蛋白质结合，但用作实验试剂。三种毒素的结合蛋白基本相同。2.毒素与结合蛋白的亲和力比毒素与受体的亲和力低100倍左右。3.三种毒素均与牛红细胞的碳酸酐酶和牛胰腺的核糖核酸酶A结合。这些结合蛋白具有高度同源的氨基酸序列，形成α-螺旋。4.结果表明，BT毒素与昆虫细胞受体结合部分的结合蛋白结合。5.这表明，三种BT毒素之一利用氨肽酶N作为受体上的家蚕中肠细胞。