Realization of a secure and safe proteinaceous-insecticide based on the directed evolution of the insecticidal toxin from soil bacterium, Bacillus thuringiensis
Realization of a secure and safe proteinaceous-insecticide based on the directed evolution of the insecticidal toxin from soil bacterium, Bacillus thuringiensis
批准号:
21310051
负责人:
SATO Ryoichi
金额:
$11.81万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
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英文摘要
It was indicated that Cry toxin mutants affinity-maturated to cadherin-like receptor is expected to be easily acquired, if mutation is introduced in loop 2 or loop 3 of Cry toxin, mutant Cry toxins are displayed on the T7 phage, and mutant-toxin-displaying phages are screened by bio-panning using cadherin-like receptor. That is to say, base technology was developed for the directed evolution of Cry toxin by which any available Cry toxins are possible to be changed to be highly active to any insect pests
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バイオパニング法を用いて選抜されたBtR175高親和性Cry1Aa変異体の解析
使用生物淘选方法筛选的 BtR175 高亲和力 Cry1Aa 突变体的分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[藤井勇樹, 他3名]
通讯作者:
他3名
Bombyx mori midgut epithelial cells' reaction induced by Cry1Aa includes both or either of osmotic swelling and apoptosis depending on toxic
Cry1Aa诱导家蚕中肠上皮细胞的反应包括渗透性肿胀和细胞凋亡两者或其中之一,取决于毒性
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Shiho Tanaka, 他2名]
通讯作者:
他2名
変異体を用いたBt菌Cry毒素のループ部位の受容体結合和性における役割の解析
使用突变体分析 Bt 细菌 Cry 毒素环位点在受体结合亲和力中的作用
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[藤井勇樹, 他5名]
通讯作者:
他5名
Cry1A毒素に対する宿主昆虫感受性の決定に関与する2分子の解析
分析参与确定宿主昆虫对 Cry1A 毒素敏感性的两个分子
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[田中詩穂, 他3名]
通讯作者:
他3名
Binding affinity improvement of the Cry1Aa toxin to the cadherin-like protein, BtR175 from Bombyx mori using phage display of loop 3 mutant toxins and bio-panning
使用环 3 突变毒素的噬菌体展示和生物淘选提高 Cry1Aa 毒素与来自家蚕的钙粘蛋白样蛋白 BtR175 的结合亲和力
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Fujii Y, Otsuki M, Hoshino Y, Sato R]
通讯作者:
Sato R
共 16 条
Evolvement of "durability mechanics" for the evaluation of shrinkage effect on performance of reinforced concrete beams
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批准号:24246077
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项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$21.8万
-
财政年份:2012
-
负责人:SATO Ryoichi
-
依托单位:
Generation of a new category, "Proteinaceous Insecticide" based on mechanisms which cause specificity diversity of Bacillus thuringiensis insecticidal protein.
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批准号:24310054
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$11.32万
-
财政年份:2012
-
负责人:SATO Ryoichi
-
依托单位:
DEVELOPMENT OF A PRACTICAL TENSILE CREEP MODEL BASED ON SIMPLE EXPERIMENTS
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批准号:24656272
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$1.5万
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财政年份:2012
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负责人:SATO Ryoichi
-
依托单位:
Development of Accurate Detection/Identification Method of Stricken Residential Buildings Using Radar Porlarimetry
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批准号:22510188
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
-
财政年份:2010
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负责人:SATO Ryoichi
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依托单位:
Durability Mechanics for High Shrinkage Concrete Members
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批准号:21246071
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$25.46万
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财政年份:2009
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负责人:SATO Ryoichi
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依托单位:
Detection and Classification of Landslide and/or Stricken Residential Area in Mountainous Region Using Radar Polarimetry
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批准号:19510183
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2007
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负责人:SATO Ryoichi
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依托单位:
Development of Expansive-High Strength Concrete and Systematic Evaluation of Structural Behavior of Reinforced Concrete Beams
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批准号:16360220
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.83万
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财政年份:2004
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负责人:SATO Ryoichi
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依托单位:
Directed evolution of the Bacillus thuringiensis insecticidal toxin for the creation of proteinaceous insecticide.
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批准号:15380228
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.96万
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财政年份:2003
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负责人:SATO Ryoichi
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依托单位:
Approach for creation of proteinaceous insecticide from Bacilhus thuringiensis delta-endotoxin by directed evolution using phage display system
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批准号:12558068
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.45万
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财政年份:2000
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负责人:SATO Ryoichi
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依托单位:
Research for a basic anti-microbial recognition system of the animal using silkworm, Bombyx mori
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批准号:10680601
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.98万
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财政年份:1998
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负责人:SATO Ryoichi
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依托单位:
Creation of proteinaceous insecticide from Bacuillus thuringlensis δ-endotoxin and single chain variable fragment
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批准号:09558076
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$5.7万
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财政年份:1997
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负责人:SATO Ryoichi
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依托单位:
Time-Dependent Deformations of Prestressed and Prestressed Reinforced High Strength Concrete Members
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批准号:08455204
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$2.24万
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财政年份:1996
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负责人:SATO Ryoichi
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依托单位:
Characterization of recognition specificity and analysis of variety of recognition proteins of BT toxin.
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批准号:08660408
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.54万
-
财政年份:1996
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负责人:SATO Ryoichi
-
依托单位:
Study on the old : strata of the accents around Tokyo
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批准号:07610422
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.22万
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财政年份:1995
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负责人:SATO Ryoichi
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依托单位:
Engeineering of delta-endotoxin from Bacillus thuringiensis to make a new specificity.
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批准号:05660385
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.34万
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财政年份:1993
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负责人:SATO Ryoichi
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依托单位:
DESIGN METHOD OF REINFORCED CONCRETE MEMBERS WITH THERMAL CRACKING
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批准号:03555105
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$1.92万
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财政年份:1991
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负责人:SATO Ryoichi
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依托单位:
STUDIES ON THE EFFECTS OF ENVIRONMENTAL CONDITION ON LONG-TERM DEFORMATION AND CRACK WIDTH OF REINFORCED CONCRETE MEMBERS
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批准号:03650389
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.28万
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财政年份:1991
-
负责人:SATO Ryoichi
-
依托单位:
A Study of Properties and Prediction of Long-term Deformation of Reinforced Concrete Members
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批准号:01550372
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.09万
-
财政年份:1989
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负责人:SATO Ryoichi
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依托单位:
海外基金