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Effects of organotin compounds and lectins on DNA

有机锡化合物和凝集素对 DNA 的影响

基本信息

批准号：
08670402
负责人：
IWAI Hideaki
金额：
$ 0.32万
依托单位：
Juntendo Univ.
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Effects of organotins arid lectins on DNA were investigated in cell lines.1) Cultured cells : leukemia and mammary tumor cell lines were maintained in RPMI-1640 medium containing 100%FBS.2) Effects of organotins and lectins on proliferation of cultured cells : organotins exhibited a specific toxic effect and the effect on lymphoid cells was stronger than on myeloid cells. Immature cells may be more sensitive to organotins than mature cells. MCF-7 (mammary tumor cell line) was proliferated at levels of 10^<-10>M order of tributyltin which cell viability was not yet impaired.3) Effects of organotins and lectins on differentiation of Friend cell the differentiation of Friend cell by DMSO was markedly inhibited by treatment with mixture of DMSO and dibutyltin and the inhibitory rate was dependent on the mixing time of DMSO and dibutyltin.4) We have isolated a novel phospholipid / ganglioside-binding protein from rabbit sera. The lectin agglutinated rabbit red cells. These lectins have collagen-like sequences in the same polypeptide chain, and are resistant to bacterial collagenase digestion.5) Ditection of extracted DNA from cultured cells and determination of 8-OHdG in urine : total DNA was extracted from cultured cells and detected by DIG method in high sensitivity. After using an ion exchange chromatography of a commercial column, the 8-OHdG was detected by HPLC with electric-chemical detector. But the recovery of added 8-OHdG in urine was about 20 - 30%, so the pretreatment method of urine must be further investigated.

本文研究了有机锡和凝集素对细胞DNA的影响：1）培养细胞：白血病细胞和乳腺癌细胞系在含100%FBS的RPMI-1640培养基中培养; 2）有机锡和凝集素对培养细胞增殖的影响：有机锡具有特异性毒性作用，对淋巴样细胞的作用强于髓样细胞。未成熟细胞可能比成熟细胞对有机锡更敏感。3<-10>）有机锡和凝集素对Friend细胞分化的影响二甲基亚砜和二丁基锡的混合物对二甲基亚砜诱导的Friend细胞分化有明显的抑制作用，抑制率与二甲基亚砜和二丁基锡的混合时间有关。4）我们从兔血清中分离到一种新的磷脂/神经节苷脂结合蛋白。凝集素能凝集兔红细胞。这些凝集素在同一条多肽链上具有胶原样序列，对细菌胶原酶消化具有抗性。5）培养细胞DNA的检测及尿中8-OHdG的测定：从培养细胞中提取总DNA，采用DIG法进行检测，灵敏度高。采用商品化的离子交换色谱柱，用高效液相色谱-电化学检测器检测8-OHdG。但尿中8-OHdG的回收率约为20 - 30%，因此尿的预处理方法有待进一步研究。