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Study on two new microglia growth factors purified from cloned culture cells

克隆培养细胞纯化两种新型小胶质细胞生长因子的研究

基本信息

批准号：
08670545
负责人：
KIKUCHI Aiko
金额：
$ 1.6万
依托单位：
National Center of Nerology and Psychiatry (NCNP)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670545/
关键词：
microglia monocyte macrophage growth factor M-CSF brain thymus myoid cell GM-CSF 培養細胞分化増殖因子アストログリア

项目摘要

We have previously found that an 80 kDa factor and a 100 kDa factor in the conditioned medium of cloned thymic myoid cells. Amino acid analysis and biological study of these factors indicate that they are different from the authentic GM-CSF and M-CSF and also different from each other. These two factors stimulate growth and differentiation of monocytic lineage cells from various organs, including the brain microglial cells. Generally, since the brain and the thymus share many biological characteristics, such as cell surface antigens and cytokines, we attempted to locate possible producer cells in the brain by using cDNA probes and antibodies specific to these two factors. We have found that the brain indeed produce two mosecules identical to the two factors derived from the thymic nyoid cels, together with other cytokines, such as M-CSF.These results suggest that development of monocytic lineage cells depend on plural growth and differentiation factors, probably a certain combination of monocytic cell growth facters are important for generation of the heterogeneity in monocytic lineage cells. We are in progress to produce their recombinant factors in large scale and to prepare the knockout mice of these factors to establish their biological significance.

我们以前发现，80 kDa的因子和100 kDa的因子克隆胸腺肌样细胞的条件培养基中。氨基酸分析和生物学研究表明，这些因子既不同于天然的GM-CSF和M-CSF，也不同于其它因子。这两种因子刺激来自各种器官的单核细胞谱系细胞（包括脑小胶质细胞）的生长和分化。一般来说，由于大脑和胸腺共享许多生物学特性，如细胞表面抗原和细胞因子，我们试图通过使用特异于这两个因子的cDNA探针和抗体来定位大脑中可能的生产细胞。我们发现脑内确实产生了两种与胸腺细胞因子相同的小分子，以及其他细胞因子，如M-CSF，这些结果提示单核细胞系的发育依赖于多种生长和分化因子，可能是某些单核细胞生长因子的组合对单核细胞系异质性的产生起重要作用。我们正在进行大规模生产重组因子，并准备这些因子的敲除小鼠，以确定其生物学意义。