Study on Gene Expressions of Two Novel Microglial Cell Growth Factors

两种新型小胶质细胞生长因子基因表达的研究

• 批准号: 10670440
• 负责人: KIKUCHI Aiko
• 金额: $ 2.11万
• 依托单位: National Center of Neuroscience, NCNP
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670440/
• 关键词: Microglia; neuronal cells; brain; monocyte; myasthenia gravis; colony stimulating factor; recombinant fator; differentiation factor; thymus; myoid cells; microglia; growth; differentiation; brain; rat; astrocyte; マクロファージ; 筋様細胞; アストロサイト /

We have previously found that thymic myoid cells produce various lympho-myelocyte stimulatory activities. We succeed in purifying two new haemopoietic factors, an 80 kDa factor and a 100 kDa factor. Those two factors are capable of inducing preferential growth of monocytic lineage cells, but differ from each other and also from the authentic macrophage colony stimulating factor and granulocyte-macrophage colony stimulating factor. We found these factors in the myasthenic thymus hyperplasia. Thereby, they appear to be responsible for intrathymic production of anti-AChR antibody. We also succeed in identifying producer cells of these two factors in the rat brain. The 80 kDa and 100 kDa factor were concomitantly produced with macrophage colocy stimulating factor. The two new factors immunopurified from the brain indeed stimulated microglial cell growth. Recombinant 80 kDa factor was obtained and study of recombinant 100 kDa factor is in progress. We are also successful in identifying the two factors in human neuronal cells.

我们之前发现胸腺肌样细胞产生各种淋巴细胞刺激活性。我们成功纯化了两种新的造血因子：80 kDa 因子和 100 kDa 因子。这两种因子能够诱导单核细胞谱系细胞的优先生长，但彼此不同，也不同于真正的巨噬细胞集落刺激因子和粒细胞-巨噬细胞集落刺激因子。我们在肌无力胸腺增生中发现了这些因素。因此，它们似乎负责胸腺内抗 AChR 抗体的产生。我们还成功地鉴定了大鼠大脑中这两个因子的生产细胞。 80 kDa 和 100 kDa 因子与巨噬细胞结肠刺激因子同时产生。从大脑中免疫纯化的两种新因子确实刺激了小胶质细胞的生长。获得重组80kDa因子，重组100kDa因子的研究正在进行中。我们还成功地鉴定了人类神经元细胞中的这两个因素。

项目成果

Kamo I., et al.: "Generation of B (B1) cells and myoid cells from thymic stromal cells"Journal of Neuroimmunoloty. 90. 91-91 (1998)

Kamo I. 等人：“从胸腺基质细胞生成 B (B1) 细胞和肌样细胞”神经免疫学杂志。

Kikuchi A., et al.: "The brain produces two novel haemopoietic factors for microglial cell growth"Journal of Neruoimmunology. 90. 49-49 (1998)

Kikuchi A. 等人：“大脑产生两种用于小胶质细胞生长的新型造血因子”《神经免疫学杂志》。

Kamo I., et al.: "Thymic myoid cells induce myasthenia gravis and hyperplasia"Cell Biology International. 24. 932-932 (2000)

Kamo I. 等人：“胸腺肌样细胞诱导重症肌无力和增生”国际细胞生物学。

Tomoyasu H., et al.: "Production of two haemopoietic factors in the hyperplastic thymus associated with myasthenia gravis"Journal of Neuroimmunology. 90. 94-94 (1998)

Tomoyasu H.等人：“与重症肌无力相关的增生性胸腺中两种造血因子的产生”神经免疫学杂志。

Tomoyasu H., et al.: "Identification of haemopoietic biglycan in hyperplastic thymus associated with myasthenia gravis"Journal of Neuroimmunology. 89. 59-63 (1998)

Tomoyasu H.等人：“与重症肌无力相关的增生性胸腺中造血双糖链蛋白的鉴定”神经免疫学杂志。