Mechanisms of production of two new microglial growth-differentiation factors from brain astrocytes and neurons

脑星形胶质细胞和神经元产生两种新的小胶质细胞生长分化因子的机制

• 批准号: 16590858
• 负责人: KIKUCHI Aiko
• 金额: $ 2.3万
• 依托单位: Institute of Animal Reproduction (2005-2006)National Center of Neurology and Psychiatry (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590858/
• 关键词: microglia; neuron; astrocyte; thymic myoid cell; monocyte; cytokine; autoimmune disease; myoastheniagrabis; 分化増殖因子; クログリア; 脳; 分化; 増殖; 胸腺; 筋様細胞; 細胞培養; 総血因子; thymic myoid cells; astrocyte; neuron; myathenia gravis; haemopoietic growth f

To elucidate the mechanism of the pathogenesis of myasthenia gravis, we studied physiological roles of thymic myoid cells. We found two new haemopoietic factors such as an 80-kDa and 100-kDa factor in myoid cells. The antibodies raised against these factors clearly localized clusters of precursor myoid cells in the hyperplastic thymi. Both factors were capable of stimulating the growth of B-cells in a synergistic fashion with other thymic cytokines. To understand the importance of numerical increase of myoid cell precursors, we studied transcription factors for muscle cells.Interestingly,the hyperplastic thymi expressed a particular type of transcription factor. These two factors were also detected in the brain cells such as glial cells and neuronal cells and play important roles in microglial cell growth and neural cell survival. In addition, we succeeded in producing recombinant products of the factors.

为了阐明重症肌无力的发病机制，我们研究了胸腺肌样细胞的生理作用。我们在肌样细胞中发现了两种新的造血因子，如80 kDa和100 kDa因子。针对这些因子产生的抗体清楚地定位了增生性胸腺中的前体肌样细胞簇。这两种因子都能与其他胸腺细胞因子协同刺激B细胞的生长。为了了解肌样细胞前体数量增加的重要性，我们研究了肌肉细胞的转录因子。有趣的是，增生的胸腺表达一种特殊类型的转录因子。这两个因子也在神经胶质细胞和神经细胞等脑细胞中检测到，并在小胶质细胞生长和神经细胞存活中发挥重要作用。此外，我们还成功地生产了这些因子的重组产品。

项目成果

Immunological and neurological roles of 80-kDa and 100-kDa haemopoietic factors.

80-kDa 和 100-kDa 造血因子的免疫学和神经学作用。

• 发表时间: 2006
• 期刊: J. Neuroimmunol. 178
• 作者: Kikuchi;A.;Tomoyasu;H.;Yamamoto;T.;Kusakabe;M.;Sakuragawa;N.;Kamo;I.
• 通讯作者: I.

Biological roles of two new haemopoietic factors in brain and thymus

脑和胸腺中两种新造血因子的生物学作用

• 发表时间: 2004
• 期刊: J. Neuroimmunol. 154
• 作者: Kikuchi;A.;Tomoyasu;H.;Yamamoto;T.;Kusakabe;M.;Sakuragawa;N.;Kamo;I.
• 通讯作者: I.

Human amnion mesenchyme cells express phenotypes of neurological progenitor cells

人羊膜间充质细胞表达神经祖细胞的表型

• 发表时间: 2004
• 期刊: J. Neuroscience Research 78
• 作者: Sakuragawa;N.;Kakinuma;K.;Kikuchi;A.;Okano;H.;Uchida;S.;Kamo;I.;Kobayashi;M.;Yokoyama;Y.
• 通讯作者: Y.

Immunological and neurological roles of 80-Kda and 100-kDa haemopoietic factors

80-Kda 和 100-kDa 造血因子的免疫学和神经学作用

• 发表时间: 2006
• 期刊: J. Immunol.
• 作者: Kikuchi;A.;Tomoyasu;H.;Kobayashi;M.;Sakuragawa;N.;Kamo;I.
• 通讯作者: I.

Analysis of cytokine network for initiation of immune responses in the hyperplastic thymus associated with myasthenia gravis

分析重症肌无力相关增生性胸腺中启动免疫反应的细胞因子网络

• 发表时间: 2005
• 期刊: J. Clinical Immunol. 193
• 作者: Kamo;I.;Kikuchi;A.;Tomoyasu;H.;Sakuragawa;N.
• 通讯作者: N.