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Study on cell proliferation in the gallbladder and bile duct epithelium with pancreaticobiliary maljinction.

胰胆错位时胆囊及胆管上皮细胞增殖的研究。

基本信息

批准号：
08671433
负责人：
ANDO Hisami
金额：
$ 1.41万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671433/
关键词：
pancreaticobiliary maljunction choledochal cyst PCNA TGF-alpha AgNOR gallbladder bile duct TGFα 膵胆管合流異常 Beal嚢胆管腺

项目摘要

We have succeeded in making a new model for the pancreaticobiliary maljunction without bile duct dilatation, and demonstrated increased cell cycle of the gallbladder and the bile duct epithelium of this model.A cat model was produced by performing choledocho-pancreatic side-to-side ductal anastomosis in nine animals. Six cats, in which the choledocho-pancreatic ducts were exposed only, served as controls. After 1 year, the common bile duct of these cats were removed and stained with anti-proliferating cell nuclear antigen (PCNA) antibody (PC10, Dako). The labeling index (LI) was determined from the percentage of positive nuclei in three microscopic fields.Histologically periductal glands are well developed in models compared to controls. In the models, the number of PCNA positive cells was significantly increased at the connecting duct between the bile duct and glands. The mean ((]SY.+-。[) standard deviation) PCNA LI was 27.2 (]SY.+-。[) 10.9% in the models and 8.3 (]SY.+-。[) 4.2% in the controls (p<0.01). Histologically invaginations of the epithelium, sacculi of Beale increased in number and periductal glands were well developed. PCNA positive cells significantly increased in the common bile duct, especially in sacculi of Beale and glands.These data clearly indicate that the cell cycle is increased not only in the gallbladder but also in the common bile duct and that excision of the bile duct is recommended in this disease.

我们成功制作了一种无需胆管扩张的胰胆管错位新模型，并证明了该模型胆囊和胆管上皮的细胞周期增加。通过对九只动物进行胆总管-胰腺侧侧吻合术制作了猫模型。六只只暴露胆总管胰管的猫作为对照。 1年后，这些猫的胆总管被切除并用抗增殖细胞核抗原（PCNA）抗体（PC10，Dako）染色。标记指数（LI）是根据三个显微镜视野中阳性细胞核的百分比确定的。与对照相比，模型中的组织学导管周围腺体发育良好。在模型中，胆管和腺体之间的连接管处的 PCNA 阳性细胞数量显着增加。模型中的平均 ((]SY.+-。[) 标准差) PCNA LI 为 27.2 (]SY.+-。[) 10.9%，对照组为 8.3 (]SY.+-。[) 4.2% (p<0.01)。组织学上皮内陷，Beale球囊数量增多，导管周围腺发育良好。 PCNA阳性细胞在胆总管中显着增加，特别是在Beale球囊和腺体中。这些数据清楚地表明，细胞周期不仅在胆囊中而且在胆总管中增加，因此建议在这种疾病中切除胆管。