权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Experiment and clinical study on pancreaticobiliary maljunction as to the influence on the bile duct dilatation

胰胆管连接不良对胆管扩张影响的实验与临床研究

基本信息

批准号：
10671172
负责人：
ANDO Hisami
金额：
$ 2.18万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671172/
关键词：
choledochal cyst pancreaticobiliary maljunction whole-mount enteric nerve system whole mount Whole mount

项目摘要

1. The aim of this study is to elucidate the pathophysiological mechanism of bile duct dilatation which is characteristic of the choledochal cyst.2. This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied. These bile ducts were fixed in Zamboni solution. Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-diotin-peroxidase method after 6- to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.3. Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.4. This distribution of nerve strands and ganglia in the bite duct wall choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacolon in patients with hypoganglionosis.

1.本研究旨在阐明以胆总管囊肿为特征的胆管扩张的病理生理机制。本研究对胆总管囊肿患者胆管壁的神经纤维和神经细胞是否异常进行了分析。本文对8例胆总管囊肿患者和3例正常胆管的胆管进行了研究。这些胆管用Zamboni液固定。在体视显微镜下切除粘膜、粘膜下层、纤维肌层，制成显示胆管外表面下肌间神经丛的全套标本。整装制剂在0.3%Triton X-磷酸缓冲液中浸泡2~3天。以抗蛋白基因产物(PGP)9.5抗体为一抗，孵育6~7d后，用免疫组织化学方法进行免疫组织化学染色。靠近外表面的纤维肌层内可见发达的肌间神经丛网络。神经节的分布清晰可见。患者的神经束与对照组无明显差异，但神经节数目减少到对照组的十分之一。胆管囊壁神经束和神经节的这种分布与神经节细胞减少症的肠壁相似，提示胆总管囊肿的胆管扩张机制与神经节细胞减少症患者的巨结肠扩张机制相同。