Study for bile duct dilatation of the choledochal cysts inferred from nerve plexus.

1. 本研究分析胆总管囊肿患者的胆管壁，观察壁内的神经纤维和神经细胞是否异常。研究了8例胆总管囊肿患者的胆管和3例未扩张的正常胆管，在体视显微镜下，通过去除粘膜、粘膜下层、纤维肌肉层，制备了胆总管外表面下的肌丛。全载制剂在0.3% Triton X-phosphate缓冲溶液中浸泡2 - 3天。孵育6 ~ 7天后，以抗蛋白基因产物（PGP） 9.5抗体为一抗，采用链霉亲和素-生物素-过氧化物酶法进行免疫组织化学染色。在近外表面的纤维肌层观察到发达的肌丛网络。神经节的分布清晰可见。患者神经束与对照组无明显差异，但神经节数量减少至对照组的十分之一。这种神经束和神经节在胆总管囊肿胆管壁的分布与神经节过低症肠壁的分布相似，提示胆总管囊肿患者胆管扩张的机制与神经节过低症患者胆管扩张的机制相同。

1. This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-biotin-peroxidase method after 6- to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.2. Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.3. This distribution of nerve strands and ganglia in the bile duct wall of choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacolon in patients with hypoganglionosis.