Study for bile duct dilatation of the choledochal cysts inferred from nerve plexus.

从神经丛推断胆总管囊肿胆管扩张的研究。

• 批准号: 12470255
• 负责人: ANDO Hisami
• 金额: $ 6.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470255/
• 关键词: choledochal cyst; pancreaticobiliary malfunction; bile duct; congenital anomaly; 神経ネットワーク; 免疫組織染色; hypoganglionosis; ヒルミュミュプルング病

1. This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-biotin-peroxidase method after 6- to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.2. Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.3. This distribution of nerve strands and ganglia in the bile duct wall of choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacolon in patients with hypoganglionosis.

1. 本研究分析胆总管囊肿患者的胆管壁，观察壁内的神经纤维和神经细胞是否异常。研究了8例胆总管囊肿患者的胆管和3例未扩张的正常胆管，在体视显微镜下，通过去除粘膜、粘膜下层、纤维肌肉层，制备了胆总管外表面下的肌丛。全载制剂在0.3% Triton X-phosphate缓冲溶液中浸泡2 - 3天。孵育6 ~ 7天后，以抗蛋白基因产物（PGP） 9.5抗体为一抗，采用链霉亲和素-生物素-过氧化物酶法进行免疫组织化学染色。在近外表面的纤维肌层观察到发达的肌丛网络。神经节的分布清晰可见。患者神经束与对照组无明显差异，但神经节数量减少至对照组的十分之一。这种神经束和神经节在胆总管囊肿胆管壁的分布与神经节过低症肠壁的分布相似，提示胆总管囊肿患者胆管扩张的机制与神经节过低症患者胆管扩张的机制相同。

项目成果

Hisami Ando, Kenichiro Kaneko, Yoshio Watanabe, Takahiko Seo,Shinsuke Katsuno, Yuj I Marui: "Treatment for pancreatic stone(protein Plug) complicated with pancreaticobiliary maljunction"Shujyutu. 54(11). 1561-1564 (2000)

Hisami Ando、Kenichiro Kaneko、Yoshio Watanabe、Takahiko Seo、Shinsuke Katsuno、Yuj I Marui：“胰腺结石（蛋白塞）并发胰胆管连接不良的治疗”Shujyutu。

Y.Watanabe, et al.: "Two-dimensional alterations of myenteric plexus in jejunoileal atresia"J. Pedeitr Surg. 36. 474-478 (2001)

Y.Watanabe 等：“空肠闭锁肌间神经丛的二维改变”J。

Hisami Ando: "Percutanenous transhepatic cholangiography and drainage for choledochal cysts in children"J Jan Soc Pediatr Radiol. 17(2). 72-77 (2001)

Hisami Ando：“经皮经肝胆管造影和引流治疗儿童胆总管囊肿”J Jan Soc Pediatr Radiol。

K.kaneko: "Pathologic changenes in the common bile duct cl an expenimental model with pancreidicobiliary maljunction without biliary-dilatation"Pediat Surg Int. 16・(1). 26-28 (2000)

K. Kaneko：“无胆管扩张的胰胆管连接不良的实验模型中胆总管的病理变化”Pediat Surg Int. 16・(1)。

Tetsuya Kaneko, Kenichiro Kaneko, Hiroyuki Sugimoto, Soichiro Inoue, Tsuyoshi Hatsuno.Kenro Sawada, Hisami Ando, Akimasa Nakao: "Intrahepatic anastomosis formation between the hepatic veins in the graft liver of the living related liver transplantation :

Tetsuya Kaneko、Kenichiro Kaneko、Hiroyuki Sugimoto、Soichiro Inoue、Tsuyoshi Hatsuno.Kenro Sawada、Hisami Ando、Akimasa Nakao：“活体相关肝移植的移植肝中肝静脉之间的肝内吻合形成：