Study for bile duct dilatation of the choledochal cysts inferred from nerve plexus.

从神经丛推断胆总管囊肿胆管扩张的研究。

• 批准号: 14571191
• 负责人: ANDO Hisami
• 金额: $ 2.18万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571191/
• 关键词: choledochal cyst; bile duct dilatation; Hirschsprung's disease; Whole-mount preparation; hypoganglionosis; hypoganglionosis; 膵胆管合流異常; 総胆管; 神経叢; 胆管

1.This study analyzed the bile duct walls of patients with choledochal cyst as to whether the nerve fibers and nerve cells in the wall are abnormal or not. Bile ducts obtained from eight patients with choledochal cyst and three normal bile ducts without dilatation were studied. Whole-mount preparations displaying the myenteric plexus running beneath the outer surface of the bile duct were made by removing the mucosa, submucosa, fibromuscular layers under a stereomicroscope. Whole-mount preparations were immersed in 0.3% Triton X-phosphate buffer solution for 2 to 3 days. They were immunohistochemically stained using the streptavidin-biotin-peroxidase method after 6-to 7-day incubation with anti-protein gene product (PGP) 9.5 antibody as the primary antibody.2.Developed networks of the myenteric plexus were observed in the fibromuscular layer near the outer surface. Distribution of the ganglia was clearly observed. Nerve strands of the patients were not significantly different from those of controls, but the number of ganglia of the patients decreased to one tenth of that of controls.3.This distribution of nerve strands and ganglia in the bile duct wall of choledochal cyst is similar to that in the intestinal wall of hypoganglionosis, which implies the mechanism of dilatation of the bile duct in patients with choledochal cyst is the same as that of megacoion in patients with hypoganglionosis.

1.本研究分析胆总管囊肿患者的胆管壁，以了解壁内的神经纤维和神经细胞是否异常。本文对8例胆总管囊肿患者的胆管和3例无扩张的正常胆管进行了研究。通过在体视显微镜下去除粘膜、粘膜下层、纤维肌层，制备显示在胆管外表面下运行的肌间神经丛的整体封片。将整体封片制备物浸入0.3% Triton X-磷酸盐缓冲液中2至3天。以抗蛋白基因产物（PGP）9.5抗体为一抗，孵育6 ~ 7天后，用链霉亲和素-生物素-过氧化物酶法进行免疫组化染色。2.在靠近外表面的纤维肌层中观察到发达的肌间神经丛网络。神经节分布清楚。胆总管囊肿的胆管壁神经束和神经节的分布与神经节细胞减少症的肠壁相似，提示胆总管囊肿患者胆管扩张的机制与巨噬细胞在神经节细胞减少症患者胆管扩张的机制相同。

项目成果

看護のための最新医学講座〔第2版〕第4巻消化管疾患 ヒルシュスプルング病と類縁疾患(千葉勉 編集)

最新护理医学课程【第2版】第4卷胃肠道疾病先天性巨结肠及相关疾病（千叶勉主编）

• 发表时间: 2005
• 作者: 安藤久實

膵胆管合流異常小児例の診断上の問題点-成人例との対比-

儿科胰胆管连接不良病例的诊断问题 - 与成人病例的比较 -

• 发表时间: 2004
• 期刊: 胆と膵 25・1
• 作者: 金子健一朗

Bile infection contributes to intrahepatic calculi formation after excision of choledochal cysts

• DOI: 10.1007/s00383-004-1253-0
• 发表时间: 2005-01-01
• 期刊: PEDIATRIC SURGERY INTERNATIONAL
• 影响因子: 1.8
• 作者: Kaneko, K;Ando, H;Ogura, Y
• 通讯作者: Ogura, Y

原 神也 他: "自己免疫性肝炎と原発性硬化性胆管炎のoverlap症候群の1例"小児科臨床. 56・9. 1849-1854 (2003)

Shinya Hara 等：“自身免疫性肝炎和原发性硬化性胆管炎重叠综合征的病例”《儿科学》56・9（2003）。

Activated Pancreatic Enzyme and Pancreatic Stone Protein (PSP/reg) in Bile of Patients with Pancreaticobiliary Maljunction/Choledochal Cysts

• DOI: 10.1007/s10620-004-9599-7
• 发表时间: 2004-11
• 期刊: Digestive Diseases and Sciences
• 影响因子: 3.1
• 作者: K. Ochiai;K. Kaneko;M. Kitagawa;H. Ando;T. Hayakawa