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細胞死からみた外科侵襲の新たな定量化法の開発

开发从细胞死亡角度量化手术侵袭的新方法

基本信息

批准号：
08671452
负责人：
山本成尚
金额：
$ 1.34万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671452/
关键词：
肝切除手術侵襲サイトカイン接着分子アポトーシス

项目摘要

我々は平成7年度から肝切除周術期における外科侵襲を炎症という観点から解析する試みを続けており、IL-6、IL-8、TNF-α等の血中炎症性サイトカイン及びICAM-1及びE-selectin等の接着分子が合併肝障害の程度を反映し、手術侵襲の程度と、更に術後臓器不全の発症及び予後と極めて密接に関連していることを報告してきた。更に、肝切除中の肝組織及び末梢血単核球のこれらの炎症性サイトカインのmRNAをRT-PCR法にて測定及び定量した結果、IL-6関しては、末梢血単核球において術後約2時間から発現が認められ、出血量、合併肝障害の程度に応じて発現が増加し、術中採取した肝組織においても、肝切除操作によって末梢単核球に比しかなり強いmRNAの発現が認められた。TNF-αに関しも、肝切除によって肝組織中のmRNA発現が増加することが示された。一方、ICAM-1mRNAに関しては、肝組織中よりも末梢単核球における発現が高い傾向が認められ、血中可溶性接着分子の由来の主座は末梢単核球であろうという従来の報告と一致した。これらの結果より、肝切除中には、血中、肝組織、末梢単核球にける炎症性サイトカイン及び接着分子が、手術侵襲並びに合併肝障害に応じて変化することが解明され、現在この結果に基づいて、肝切除術中における肝組織、及び末梢血単核球におけるapoptosis誘導を検索中である。TNF-αmRNAが肝組織中において強く発現していることから恐らくKupffer細胞からTNF-αが分泌され、肝実質細胞においてapoptosisが誘導される可能性が示唆され、現在apoptosisの程度と手術侵襲の程度を比較検討中であり、apoptosisという現象自体が手術侵襲の新しい評価法となり得ることが示唆された。更に、最近NOとapoptosis誘導との関連が注目されているが、肝切除中にiNOSmRNAが発現することを確認している。

During the period of hepatectomy week in Pingcheng, we analyzed the clinical features of inflammatory diseases, such as inflammation, IL-6, IL-8, TNF- α, ICAM-1 and E-selectin in blood, and then the molecular markers reflect the degree of liver damage and the degree of hand invasion. In the future, the symptoms of mechanical insufficiency and the close contact between the patients and the report will be very important in the future. During hepatectomy, liver tissue and peripheral blood nuclear globules were detected during hepatectomy, inflammatory liver tissue and peripheral blood nuclear globules were detected by mRNA RT-PCR method, IL-6 and peripheral blood nuclear globules were detected at about 2 hours after hepatectomy, blood loss, severity of liver damage and liver damage were detected, and liver tissue samples were collected during surgery. The procedure of hepatectomy showed that the peripheral nuclear ball was stronger than the mRNA. The expression of TNF- α and mRNA in liver tissue after hepatectomy and hepatectomy was detected. On the other hand, the ICAM-1mRNA antigen, the peripheral nuclear globule in the liver tissue, the high temperature, the soluble molecule in the blood, the main seat, the nuclear globule in the end, the nuclear globule in the liver tissue, the peripheral nuclear globule in the liver tissue, the peripheral nuclear globule in the liver tissue, the peripheral nuclear globule in the liver tissue and the soluble molecules in the blood. During hepatectomy, serum, liver tissue, peripheral nuclear globules were used to detect inflammatory response and molecular markers, surgical invasion and combination of liver damage, liver tissue, liver tissue, peripheral blood, liver tissue, liver tissue, peripheral blood, liver tissue, liver tissue and peripheral blood nuclear globule during hepatectomy. The results of liver cancer, liver tissue, liver tissue, liver tissue and peripheral blood nuclear globule during hepatectomy were analyzed in order to understand the results of liver tissue, liver tissue and peripheral blood nuclear globule during hepatectomy. now, the results of hepatectomy, liver tissue, liver tissue, peripheral blood nuclear globule and peripheral blood nuclear globule were detected in apoptosis guidance. In the liver tissue of TNF- α mRNA, there is a strong fear that TNF- α is secreted by Kupffer cells, and that the possibility of surgical invasion is higher than that of hand invasion. The current degree of apoptosis is higher than that of surgical invasion. Recently, NO apoptosis guidance has been paying close attention to the diagnosis of liver cancer, and iNOSmRNA liver surgery has shown that it is important to confirm the disease.