Neurotrophic Effects of Cytokine-activated Astrocytes.-Basic research for Elucidation of Interaction between Neuron and Glia, and Restoration of Damaged Neuronal Function-

细胞因子激活的星形胶质细胞的神经营养作用。-阐明神经元和神经胶质细胞之间的相互作用以及受损神经元功能恢复的基础研究-

• 批准号: 08671615
• 负责人: YOSHIDA Kazunari
• 金额: $ 1.41万
• 依托单位: KEIO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671615/
• 关键词: cytokine; neuron; astrocyte; neurotrophic factor; cell adhesion molecule

We have demonstrated that production and secretion of neurotrophic factors such as nerve growth factor (NGF) from astrocytes was enhanced by cytokines such as fibroblast growth factor(FGF), interleukin-1beta(IL-1beta), tumor necrosis factor-alpha(TNF-alpha), epidermal growth factor(EGF), transforming growth factor-beta1(TGF-beta1), which are likely to be present in the brain following injury.We have demonstrated new knowledge mentioned below, from 1996- to 1998.1. IL-1beta, TNF-alpha and EGF induced selective increase of high-molecular-weight bFGF isoforms in cultured rat hippocampal astrocytes.2. IL-1beta and TNF-alpha co-operatively enhanced a novel trophic activity of astrocytes for pontine cholinergic neurons.3. TGF-beta1 enhanced expression of NCAM in cultured rat hippocampal astrocytes.4. Hepatocyte growth factor(HGF) receptor, c-Met was expressed in cultured rat hippocampal astrocytes. Cytokines enhanced expression of c-Met protein in cultured rat hippocampal astrocytes.

我们已经证明，成纤维细胞生长因子（FGF）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、表皮生长因子（EGF）、转化生长因子等细胞因子可增强星形胶质细胞神经营养因子（如神经生长因子（NGF））的产生和分泌。 因子-β1（TGF-β1），损伤后很可能存在于大脑中。从1996年到1998年，我们已经证明了下面提到的新知识。1。 IL-1β、TNF-α和EGF诱导培养的大鼠海马星形胶质细胞中高分子量bFGF亚型选择性增加。2． IL-1β和TNF-α协同增强星形胶质细胞对脑桥胆碱能神经元的新型营养活性。 3． TGF-β1增强大鼠海马星形胶质细胞NCAM表达。 4．肝细胞生长因子（HGF）受体c-Met在培养的大鼠海马星形胶质细胞中表达。细胞因子增强培养的大鼠海马星形胶质细胞中 c-Met 蛋白的表达。

项目成果

Shimazaki K,Inaba M,Yoshida K,et al.: "Regulation of membranous proteins expression by cytokines in cultured rat hippocampal astrocytes"Society for Neuroscience Abstracts. 23. 883 (1997)

Shimazaki K、Inaba M、Yoshida K 等人：“培养的大鼠海马星形胶质细胞中细胞因子对膜蛋白表达的调节”神经科学学会文摘。

上口裕之、吉田一成、島崎賢仁ら: "培養アストロサイトにおけるbFGF isoformの発現パターンおよび細胞内局在におよぼすサイトカインの影響" 神経組織の成長・再生・移植. 8. 15-16 (1996)

Hiroyuki Kamiguchi、Kazunari Yoshida、Kenji Shimazaki 等人：“细胞因子对培养的星形胶质细胞中 bFGF 异构体的表达模式和亚细胞定位的影响”《神经组织的生长、再生和移植》（1996 年）。

Sasaki H., Yoshida K., Inaba M., Ikeda E., Kagami H., Shimazaki K., Kondo A., Otani M.and Kawase T.: "Expression of the neural adhesion molecule (NCAM) in the astrocytic tumors : an inverse correlation with malignancy." Society for Neuroscience. 23 (Abstr

Sasaki H.、Yoshida K.、Inaba M.、Ikeda E.、Kagami H.、Shimazaki K.、Kondo A.、Otani M.和 Kawase T.：“神经粘附分子 (NCAM) 在星形细胞肿瘤中的表达

Inaba M., Yoshida K., Sasaki H., Kagami H., Shimazaki K., Otani M.and Kawase T.: "Expression of NCAM and polysialic moiety in cultured neurons." Restorative Neurology and Neuroscience. 11. 234-235 (1997)

Inaba M.、Yoshida K.、Sasaki H.、Kagami H.、Shimazaki K.、Otani M.和 Kawase T.：“培养神经元中 NCAM 和多唾液酸部分的表达”。

Kagami H., Yoshida K., Wakamoto H., Sasaki H., Inaba M., Shimazaki K.and Kawase T.: "Expression of acidic fibroblast growth factor in human schwannomas and its biological activities." Society for Neuroscience. 23 (Abstracts). 884 (1997)

Kagami H.、Yoshida K.、Wakamoto H.、Sasaki H.、Inaba M.、Shimazaki K.和 Kawase T.：“酸性成纤维细胞生长因子在人神经鞘瘤中的表达及其生物活性。”