Molecular analysis of key factors involved in the mechanism of invasion in gliomas

胶质瘤的恶性行为以其侵袭性为特征，其生物学机制引起了科学家的极大兴趣，其生物学影响因素尚不清楚。肝细胞生长因子(HGF)具有多种生理功能，其受体c-Met被认为在多种恶性肿瘤的病理过程中起决定性作用。HGF和c-met基因在胶质瘤细胞系中同时表达已有报道，但对其在胶质瘤组织中的表达特征知之甚少。1)培养的星形胶质细胞表达c-Met蛋白；2)多种神经营养因子上调NGF的表达；3)c-Met蛋白的表达与低级别星形细胞瘤的复发有关，与胶质瘤的侵袭机制有关，而与恶性进展无关；细胞黏附分子也是脑肿瘤恶性潜能的关键因素之一。使用神经胶质瘤细胞系的证据表明，神经细胞黏附分子(NCAM)与迁移和侵袭潜能有关。他研究了NCAM在星形细胞肿瘤中的表达，阐明了每种NCAM亚型在组织学恶性进展中的比例降低。换言之，NCAM表达降低可能导致生物恶性的发生。我们还确定了作为神经干细胞成熟关键因素的武藏表达的表达及其相互关系。

The malignant behavior of glioma is characterized by its invasiveness and its biological mechanism is of significant interest among scientists, Key factors contributing to its biology is yet to be determined. Hepatocyte growth factor (HGF) which has various physiological functions, and its receptor c-Met are thought to be determinant in the pathological processes of various malignancies. Concominant expression of HGF and c-met genes has been reported in glioma cell lines but little is known about the characteristics in glioma tissue. We made an analysis to elucidate the function of c-Met in glioma tissues which are concluded that 1) cultured astrocytes express c-Met protein and, 2) various neurotropic factors elevate the expression of NGF, 3) expression of c-Met protein interrelates with the recurrence of low-grade astrocytomas, and interrelation with the mechanism of invasion of glioma and not the malignant progression.Cell adhesion molecules are also among the key factors of the malignant potential of brain tumors. Evidence from usage of glioma cell lines has shown correlation of neural cell adhesion molecule (NCAM) with the migratory and invasive potential nature. He studied the expression of NCAM in astrocytic tumors which elucidated that each NCAM isoform decreased its proportion to the progression of the histological malignancy. In other words, reduced NCAM expression might result in development of biological malignancy. We also ascertained the expression and the relation of Musashi expression which is known as a key factor in maturation of the neural stem cell.