Pathological study on the prevention of osteonecrosis of the femoral head in spontaneously hypertensive rats.

预防自发性高血压大鼠股骨头坏死的病理学研究

• 批准号: 08671669
• 负责人: HIRANO Toru
• 金额: $ 1.6万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671669/
• 关键词: Spontaneously hypertensive rats; Femoral head; Dsteonecrosis; Behavior analysis; Standing

Osteonecrosis of the femoral head which resembled Perthes'-disease occurs naturally in spontaneously hypertensive rats (SHRs). A similar lesion could be induced by standing in Wistar Kyoto rats (WKYs). Standing is one of major factors by which the femoral head suffers mechanical stress. To clarify the pathogenesis of the osteonecrosis in rats would contribute for prevention and treatment of Perthes' disease. The purpose of this study is to investigate the participation of the mechanical stress on the femoral head in the occurrence of osteonecrosis of the femoral head.In this experiment, custom-designed cages were prepared, which were 27 cm of height (high cage) and 10 cm of height (low cage). SHRs and WKYs at 5 weeks after birth were fed in these cages. In high cage group, a feed box and a drinking aperture were located high enough so that rats had to stand in feeding. To evaluate their activity, behavior was recorded on video tape using a camera with notovision. The rats were very act … More ive at night and stood up a total of around 2 hours each day in high cage group. The rats in low cages could not stand up. For pathological study, bilateral femurs were resected under ether anesthesia at 9, 12 and 15 weeks of age. After soft X-ray photos taken, thin paraffin-embedded sections of the femoral heads were stained with hematoxylin eosin.In SHRs, the incidence of the osteonecrosis of high cage group was slightly higher than that of low cage group. The difference between both groups was significant in WKYs. The osteonecrosis rarely occurred in low cage group, however, it appeared frequently at 15 weeks in high cage group. WKYs at 15 weeks in high cage group showed localized cartilaginous abnormalities of the growth plate like SHRs. The abnormalities were remarkable in the lateral of the femoral head where the mechanical stress centered and the feeding vessels penetrated into. These results suggest that the repeated mechanical stress playd an important role in the occurrence of osteonecrosis of the femoral head. Less

自发性高血压大鼠（SHR）的股骨头坏死类似于Perthes病。在Wistar京都大鼠（WKY）中，通过站立可诱导类似的损伤。站立是股骨头承受机械应力的主要因素之一。阐明大鼠股骨头坏死的发病机制将有助于预防和治疗Perthes病。本研究的目的是探讨股骨头上的机械应力在股骨头坏死发生中的参与，在本实验中，制备定制的融合器，其高度为27 cm（高融合器）和10 cm（低融合器）。出生后5周的SHR和WKY在这些笼中饲养。高笼组设置饲料箱和饮水口，大鼠必须站立进食。为了评估他们的活动，他们的行为被记录在录像带上，使用一台带notovision的相机。老鼠们非常活跃， ...更多信息 在高笼组中，在夜间饲养并且每天总共站立约2小时。低矮笼子里的老鼠无法站起来。在9、12、15周龄时，在乙醚麻醉下切除双侧股骨进行病理学研究。软X线摄片后，石蜡包埋切片，苏木精-伊红染色，高Cage组骨坏死发生率略高于低Cage组。两组间WKY差异有统计学意义。低笼组骨坏死发生率低，而高笼组骨坏死多发生在15周。15周时高笼组大鼠生长板局部软骨异常，与SHR相似。股骨头外侧的异常最为明显，此处为机械应力集中区，供血血管穿入。提示反复机械应力在股骨头坏死的发生中起重要作用。少

项目成果

Toru Hirano et al.: "Osteonecrosis of the femoral head in spontaneously hypertensive rats [Japanese]" Seikeigeka. 47. 491-496 (1996)

Toru Hirano 等人：“自发性高血压大鼠股骨头坏死[日语]”Seikeigeka。

平野 徹: "高血圧自然発症ラット(SHR)にみられる大腿骨頭壊死の病態" 整形外科. 47. 491-496 (1996)

Toru Hirano：“在自发性高血压大鼠 (SHR) 中观察到的股骨头坏死的病理学”《骨科》47. 491-496 (1996)。

Toru Hirano et al.: "Characteristics of blood vessels feeding the femoral head liable to osteonecrosis in spontaneously hypertensive rats" Calcif.Tissue Int.58. 201-205 (1996)

Toru Hirano 等人：“自发性高血压大鼠股骨头供血容易发生骨坏死的血管特征”Calcif.Tissue Int.58。

Toru Hirano et al.: "Structural changes in blood vessels entering the growth plate during growth" Int.Orthop.22 : (in press). (1998)

Toru Hirano 等人：“生长过程中进入生长板的血管的结构变化”Int.Orthop.22：（印刷中）。

Toru Hirano: "Structural changes in blood vessels entering the growth plate during growth in rats." International Orthopaedics. 22(印刷中). (1998)

Toru Hirano：“大鼠生长过程中进入生长板的血管结构变化。”国际骨科 (1998)。