EFFECTS OF VOLATILE ANESTHETICS ON FUNCTION OF SARCOPLASMIC RETICULUM IN FETAL AND ADULT MOUSE MYOCYTES

我们研究了挥发性麻醉剂对胎儿和成人心肌细胞肌浆网功能的影响。1.挥发性麻醉剂对分离的成年兔心室肌细胞和培养的小鼠胚胎心肌细胞收缩的影响；2.挥发性麻醉剂对分离的兔心室肌细胞和培养的小鼠胚胎心肌细胞内钙离子浓度的影响；3.挥发性麻醉剂对分离的大鼠心室肌细胞和培养的胎鼠心肌细胞[Ca_(2+)]i的影响。所有的挥发性麻醉剂都能降低收缩压，氟烷是一种比异氟醚和七氟醚更有效的心功能抑制药。我们的结果提示，挥发性麻醉剂抑制成人心肌细胞肌浆网的功能。

We studied the effects of volatile anesthetics on function of sarcoplasmic reticulum in fetal and adult myocytes as below. 1.effects of volatile anesthetics on contractility in isolated adult rabbit ventricular myocytes and cultured mouse fetal myoytes, 2.effects of volatile anesthetics on intracellular calcium concentration ([Ca_<2+>]i) in isolated rabbit ventricular myocytes and cultured mouse fetal myoytes, 3.effects of volatile anesthetics on [Ca_<2+>]i in isolated rat ventricular cells and cultured fetal embric myoytes with or without ryanodine.All volatile anesthetics decreased contracrility in a dose dependent manner in both neonatal and adult myocytes. All volatile anesthetics also decreased systolic [Ca_<2+>]i. Halothane was a more potent depressant of cardiac function than isoflurane and sevoflurane. Our results suggested that volatile anesthetics inhibited function of the sarcoplasmic reticulum in adult myocytes.