EFFECTS OF VOLATILE ANESTHETICS ON FUNCTION OF SARCOPLASMIC RETICULUM IN FETAL AND ADULT MOUSE MYOCYTES

挥发性麻醉剂对胎鼠和成年小鼠肌浆网功能的影响

• 批准号: 08671722
• 负责人: NAKAMURA Toshihiro
• 金额: $ 1.41万
• 依托单位: YAMANASHI MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671722/
• 关键词: SARCOPLASMIC RETICULUM; CALCIUM; VOLATILE ANESTHETICS; カルシウム; 収縮力; 筋小包体; 細胞内カルシウム濃度

We studied the effects of volatile anesthetics on function of sarcoplasmic reticulum in fetal and adult myocytes as below. 1.effects of volatile anesthetics on contractility in isolated adult rabbit ventricular myocytes and cultured mouse fetal myoytes, 2.effects of volatile anesthetics on intracellular calcium concentration ([Ca_<2+>]i) in isolated rabbit ventricular myocytes and cultured mouse fetal myoytes, 3.effects of volatile anesthetics on [Ca_<2+>]i in isolated rat ventricular cells and cultured fetal embric myoytes with or without ryanodine.All volatile anesthetics decreased contracrility in a dose dependent manner in both neonatal and adult myocytes. All volatile anesthetics also decreased systolic [Ca_<2+>]i. Halothane was a more potent depressant of cardiac function than isoflurane and sevoflurane. Our results suggested that volatile anesthetics inhibited function of the sarcoplasmic reticulum in adult myocytes.

我们研究了挥发性麻醉药对胎儿和成人心肌细胞肌浆网功能的影响。1.挥发性麻醉药对离体成年兔心室肌细胞和培养的小鼠胎儿心肌细胞收缩性的影响2.挥发性麻醉药对细胞内钙离子浓度的影响（[Ca <2+>]i）的变化，3.吸入麻醉药对[Ca_（2+）]的影响所有的挥发性麻醉药都能剂量依赖性地降低新生和成年心肌细胞的收缩性。所有吸入麻醉药均降低收缩期[Ca <2+>]i。氟烷对心功能的抑制作用强于异氟烷和七氟烷。我们的研究结果表明，挥发性麻醉剂抑制成人心肌细胞肌浆网的功能。