Interphase cytogenetic analysis in adrenal neoplasms

我们研究了43例原发性醛固酮增多症腺瘤和24例库欣综合征腺瘤的DNA含量和细胞核分级。与正常肾上腺或库欣综合征腺瘤相比，原发性醛固酮增多症的腺瘤在流式细胞仪直方图上的4C峰明显更宽(p&lt；0.0001)。67例肾上腺腺瘤中，30例为二倍体，27例为四倍体，8例为非整倍体，17例正常肾上腺均为二倍体。DNA倍体与临床症状显著相关，即原发性醛固酮增多症腺瘤的四倍体DNA直方图比例高于库欣综合征腺瘤(p=0.0004)。原发性醛固酮增多症的腺瘤的核级别(III和IV级)显著高于库欣综合征的肿瘤(P=0.038)。在57例非整倍体肿瘤中，用四聚体…也观察到dna倍体与核分级显著相关。更多的类肿瘤通常表现为最高的核级别(p=0.042)。对12例肾上腺皮质腺瘤(库欣综合征5例，原发性醛固酮增多症7例)进行了3、7、8、11、12和17号染色体的流式细胞仪DNA倍体和间期荧光原位杂交(FISH)分析。DNA倍体分析发现8例二倍体肿瘤和4例四倍体肿瘤，未发现DNA异倍体。四倍体仅见于原发性醛固酮增多症的腺瘤，而FISH分析显示染色体畸变率与DNA倍体有一定差异。原发性醛固酮增多症的腺瘤在分析的两条或两条以上染色体中显示四体，与继承人DNA倍体无关。与原发性醛固酮增多症的腺瘤相比，S综合征腺瘤的染色体异常较少。然而，在库欣综合征的5个AGA中，有3个具有8号染色体三体，此外，患有原发性醛固酮增多症的腺瘤经常表现为同一染色体的三体。这些发现表明，患有原发性醛固酮增多症的腺瘤通常具有异常的(四体)干线，8号染色体的三体可能在肾上腺皮质腺瘤的发生发展中起重要作用。较少

We investigated the flow cytometric DNA content and nuclear grade in 43 adenomas associated with primary aldosteronism and 24 adenomas with Cushing's syndrome. Adenomas with primary aldosteronism had a significantly wider 4C peak on flow cytometric histogram than normal adrenal glands or adenomas with Cushing's syndrome (p<0.0001, respectively). Flow cytometric analysis showed diploidy in 30, tetraploidy in 27 and aneuploidy in 8 of the 67 adenomas, and all 17 normal adrenal glands showed diploidy. A significant correlation was noted between DNA ploidy and clinical syndrome, i.e., a larger proportion of adenomas with primary aldosteronism showed a tetraploid DNA histogram compared with adenomas with Cushing's syndrome (p=0.0004). Adenomas with primary aldosteronism had a significantly higher nuclear grade (III and IV) than did tumors with Cushing's syndrome (p=0.038). A significant correlation was also observed between DNA ploidy and nuclear grade in 57 nonaneuploid tumors, with tetrap … More loid tumors often showing the highest nuclear grade (p=0.042). Our results showed that adrenal cortical adenomas associated with primary aldosteronism often revealed severe nuclear pleomorphism, indicating that nuclear pleomorphism might be due to a tetraploid stemline.DNA ploidy by flow cytometry and interphase fluorescence in situ hybridization (FISH) analysis of chromosome 3, 7, 8, 11, 12, and 17 were performed on 12 adrenal cortical adenomas (5 with Cushing's syndrome and 7 with primary aldosteronism). DNA ploidy analysis revealed 8 diploid and 4 tetraploid tumors, and DNA aneuploidy was not found in this study. Tetraploidy was found only in adenomas with primary aldosteronism, On the other hand, FISH analysis revealed some discrepancies between the chromosomal aberrations and DNA ploidy. Adenomas with primary aldosteronism showed tetrasomy in two or more chromosomes analyzed, irrespective of heir DNA ploidy. Adenomas with Gushing' s syndrome had fewer chromosomal abnormalities compared with tumors with primary aldosteronism. However, three of five AGAs with Cushing's syndrome had trisomy of chromosome 8, and in addition, adenomas with primary aldosteronism frequently showed trisomy of the same chromosome. These findings suggest that adenomas with primary aldosteronism may often have an aberrant (tetrasomic) stemline, and that trisomy of chromosome 8 may play an important role in the development of adrenal cortical adenoma. Less