DNA microarray analysis in transgenic mouse prostate cancer model

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1.To test if the mouse β-microseminoprotein/94-amino acid prostate secretory protein (β-MSP/PSP94) gene is prostate tissue-specific, we generated two antibodies against mouse β-MSP/PSP94. Results from this study have led to the possibility of utilizing β-MSP/PSP94 as a targeting agent specifically to the prostate in a mouse experimental model.2.To study the genetic and molecular profiles of the transgenic mouse adenocarcinoma of the prostate (TGMAP) directed by the PSP94 gene promoter/enhancer region, we performed flow cytometry and cDNA microarray analyses.(1)Flow cytometry study of DNA ploidy and proliferation rate demonstrated that the majority of TGMAP cells were aneuploid and have a high proliferative activity (average %S+% G2M:23.0 %).(2)TGMAP model demonstrated an unequally high abundance of neuroendocrine (NE)-related genes, and three categories of NE gene groups were further classified : NE related genes ; Ca++ metabolism, Na+,K+ channels ; cell differentiation, morphogenesis. TGMAP mice demonstrated abundant up-regulation of regulator factors involved in the signal transduction of tumor development and progression, cell membrane matrix/adhesion molecules, and tumor-associated growth factors.3.We assessed the usefulness of three-dimensional ultrasound microimaging for measuring tumor progression in the TGMAP model. The application of three-dimensional ultrasound imaging to prostate cancer in mice showed advantages, such as high spatial resolution, and fast and uncomplicated protocols that will likely enable ultrasound to become a new microimaging modality for mouse preclinical trial studies.