Molecular biology of the degenerative and regenerative mechanisms in the inner ear sensory cells

内耳感觉细胞退行性和再生机制的分子生物学

• 批准号: 08671966
• 负责人: TAKUMIDA Masaya
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671966/
• 关键词: inner ear; vestibular organ; cytoskeleton; nitric oxide; otoconia; pathology; molecular biology; カルシウム; モルモット; X線マイクロアナライザー

The aim of this study is to investigate the degenerative and regenerative mechanisms of inner ear sensory cell in to develop the new treatments for the inner ear diseases.The effect of ototoxic drugs on the cytoskeletal organization of vestibular sensory cells was investgated. The cytoskeletons were noted to have degenerated following ototoxic drug intoxication. Such degeneration was not linked with primary mitochondrial damage, but closely releated to subsequent degeneration of membrane-bound organelles. These findings suggest that cytoskeletons work closely together to maintain the structural integrity of the membrane-bound organelles, which can be altered by ototoxic drugs before the degeneration of membrane-bound organells.In the inner ear, constitutive NOS has been identified. NO mediates neurotransmission and play an important role in regulating vascular tone, and in maintaining endolymph and ion homeostasis. In contrast, enhanced NO production by inducible NOS and subsequent for … More mation of peroxynitrite is likely to be an important factor responsible for pathological insult of the vestibuli and removal of NO and/or O_2 to prevent formation of peroxynitrite or removal of peroxynitrite itsel may be beneficial treatment for vestibular diseases.The otoconial dynamics have been investigated using scanning electron microscopy, x-ray microanalysis and several kinds of Ca tracers. Dynamic exchange and/or uptake of Ca ions may occur even in adult animals. The giant otoconia are suggested to be formed mainly by dissolution of normal otoconia due to the loss of environmental calcium, followed by recrystallization as giant crystals. These phenomena seemed to be closely related to the otoconial dynamics which may regulate calcium ion homeostasis of endolymph.These results were presented at meetings (Sendai Symposium 96 and 97,5th and 6th meetings of Otological Society of Japan, 55th and 56th meetings of Japan Society for equilibrium Research) and were presented by 21 papers. Less

这项研究的目的是研究内耳感觉细胞的退化性和再生机制，以开发内耳疾病的新治疗方法。耳毒性药物对前庭感觉细胞细胞骨架组织的影响。注意到在耳毒性药物中介绍后，细胞骨架已退化。这种变性与主要的线粒体损伤无关，而与随后的膜结合细胞器变性密切相关。这些发现表明，细胞骨架紧密合作，以维持膜结合的细胞器的结构完整性，在膜结合膜结合细胞器之前，可以通过耳毒性药物改变膜的结构性。没有介导神经传递并在控制血管张力以及保持内淋巴和离子稳态方面发挥重要作用。相比之下，没有通过诱导的NOS和随后的……更多的过氧亚硝酸盐产生的产生可能是负责病理学损害的重要因素，并且去除NO和/或O_2可能会导致过氧氮或去除过氧一度的硝酸盐对其进行过多的治疗，从而对其进行了分配的治疗。显微镜，X射线微分析和几种CA示踪剂。即使在成年动物中，动态交换和/或摄取也可能发生。建议巨大的耳鼻菌主要是由于环境钙的丧失而主要是通过溶解正常的奥托托西病来形成的，其次是重结晶为巨晶体。这些现象似乎与可能调节内淋巴的钙离子稳态的耳尾动力学密切相关。这些结果在会议上（日本耳尾学会的第96和第96和97,5和第6届会议，日本平衡研究协会的第55和第56届会议）提出，并由21篇论文提出。较少的

项目成果

Takumida M,Zhang DM: "Electron probe x-ray microanalysis of otoconia in guinea pig inner ear : A comparison between young and old animals." Acta Otolaryngol (Stockh). 117. 529-537 (1997)

Takumida M，Zhang DM：“豚鼠内耳耳石的电子探针 X 射线微分析：年轻和年老动物之间的比较。”

Takumida M., Zhang DM, Yajin K., Harada Y.: "Polychromatic labeling of otoconia for the investigation of calcium turnover" ORL. 59. 4-9 (1997)

Takumida M.、Zhang DM、Yajin K.、Harada Y.：“耳石多色标记用于钙周转研究”ORL。

Tanigawa T,Miyawaki H,Takumida M,et al.: "Motility of isolated vestibular hair cells" Equilibrium Res. 55. 20-25 (1996)

Tanikawa T、Miyawaki H、Takumida M 等人：“分离的前庭毛细胞的运动性”平衡研究。

Takumida M., Zhang DM: "Electron probe x-ray microanalysis of otoconia in the guinea pig inner ear" ORL. 59. 187-192 (1997)

Takumida M.，Zhang DM：“豚鼠内耳耳石的电子探针 X 射线微分析”ORL。

Takumida M, Zhang DM, Yajin K, Harada Y:"Effect of streptomycin on the otoconial layer of the guinea pig." ORL. 59・5. 263-268 (1997)

Takumida M、Zhang DM、Yajin K、Harada Y：“链霉素对豚鼠耳圆锥层的影响”59・5（1997）。