Role of heat shock proteins in oral mucosal diseases and focal in fection

热休克蛋白在口腔粘膜疾病和感染中的作用

• 批准号: 08672168
• 负责人: ANDO Tomohiro
• 金额: $ 1.09万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672168/
• 关键词: Heat shock protein; focal infection; Pustulosis palmaris et plantoris; 歯周病; 口腔粘膜疾患; 掌蹠膿胞症

Heat shock proteins (HSP) are highly conserved immunogenic molecules that are induced by a variety of stresses. Structural homology between microbial and human HSP has been postulated to be a basis for autoimmunization in chronic inflammatory diseases. We estimated by ELISA titration the antibody levels to GloEL/GroES,Mycobacterium bovis BCG HSP65 and recombinant human HSP60 in sera of patients with palmoplantar pustulosis (PPP). In comparison with healthy controls (n=22,0.218(]SY.+-。[)0.176), serum anti GroEL/GroES lgG antibodies were elevated in PPP (n=43, 0.342(]SY.+-。[)0.246, p<0.05), but anti M.bovis HSP65 and r-human HSP60 antibodies were not significant difference. There were a correlation between levels of anti GroEL/GroES antibody and r-human HSP60. In PPP, as a focal infectious disease, bacterial HSP might be a antigen, but human HSP60 not be autoantibody.

热休克蛋白(HSP)是一种高度保守的免疫原性分子，可在各种应激条件下诱导产生。微生物和人类热休克蛋白的结构同源性被认为是慢性炎症性疾病自身免疫的基础。用酶联免疫吸附试验(EL ISA)滴定方法检测掌跖脓疱病(PPP)患者血清中抗GloEL/GROES、牛分枝杆菌、卡介苗HSP65和重组人HSP60抗体水平。与健康对照组(n=22，0.218(SY+-))比较。[0.176]，PPP患者血清抗GroEL/GroES-IgG抗体升高(n=43，0.342)。抗牛分枝杆菌热休克蛋白65抗体与r-人热休克蛋白60抗体无显著差异。抗GroEL/GroES抗体水平与r-人HSP60相关。在PPP中，作为一种局灶性传染病，细菌热休克蛋白可能是一种抗原，而人热休克蛋白60不是自身抗体。