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An immunohistochemical study of the relationship between periodontopathogens and focal infection

牙周病原菌与病灶感染关系的免疫组化研究

基本信息

批准号：
12671854
负责人：
ANAN Hisashi
金额：
$ 2.24万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671854/
关键词：
periapical lesion focal infection P. gingivalis mixed infection IL-1β bone resorption TGF-β1 bone formation 歯性病巣感染オフロキサシン IL-1α 骨芽細胞 IL-1発現細胞マクロファージ T細胞顎下リンパ節心内膜

项目摘要

It is speculated that focal infection of oral origin may derive from periodontal pockets and periapical lesions. Porphyromonas gingivalis is believed to closely associated with adult periodontitis. It was recently reported that P. gingivalis was isolated from periapical lesions. In this study, we determined the ability of combination of periodontopathogenic microorganisms to cause tissue destruction in a murine abscess model. Although all bacterial combinations used in this study produced larger abscess than did monoinfection of each bacterium, the combination of P. gingivalis and B. forsythus showed a synergistic effect on an abscess formation. Since these two bacteria have been frequently found together in lesions of periodontitis, it has been suggested that the significance of their co-infection in the progression of periodontitis. On the other hand, the combination of P. gingivalis and a mixed flora of bacteria within the root canal enhanced bone destruction around the root apex, and progressed the expansion of submaxillary lymph nodes in a rat model system of apical periodontitis. During the bone resorption phase, numerous macrophages expressing IL-1β were observed adjacent to many osteoclasts that contained large cytoplasms. On the contrary, after the application of an antibacterial agent (ofloxacin) to experimentally induced periapical lesions, a significant increase of macrophages expressing TGF-β1 and extensive bone formation was seen in the periapical lesions. These results suggest that mixed infection of P. gingivalis and the intra-root canal bacteria may be involved in progression of apical periodontitis, and macrophages expressing TGF-β1 may play an important role in the activation of new bone formation during the healing process of apical periodontitis.

推测口腔局灶性感染可能来源于牙周袋和根尖周病变。牙龈卟啉单胞菌被认为与成人牙周炎密切相关。最近有报道称，从根尖周病变中分离到牙周炎杆菌。在这项研究中，我们确定了牙周致病微生物组合在小鼠脓肿模型中造成组织破坏的能力。虽然本研究中使用的所有细菌组合产生的脓肿比每种细菌单独感染时产生的脓肿更大，但牙龈假单胞菌和福赛斯杆菌的组合在脓肿形成方面显示出协同作用。由于这两种细菌经常在牙周炎的病变中被发现，因此它们的联合感染在牙周炎的进展中具有重要意义。另一方面，在大鼠根尖周炎模型系统中，牙龈假单胞菌与根管内混合菌群的结合增强了根尖周围的骨破坏，并促进了颌下淋巴结的扩张。在骨吸收阶段，大量表达IL-1β的巨噬细胞与许多破骨细胞毗邻，这些破骨细胞含有较大的细胞质。相反，应用抗菌剂氧氟沙星诱导的根尖周病变后，表达转化生长因子-β1的巨噬细胞显著增加，并可见广泛的骨形成。提示Pg和根管内细菌的混合感染可能参与了根尖周炎的进展，表达转化生长因子-β1的巨噬细胞可能在根尖周炎愈合过程中新骨形成的激活中起重要作用。