Search for invasion-and metastasis-related genes in human oral cancer

自分泌运动因子（Autocrine motility factor，AMF）是一种由肿瘤细胞分泌的55 kDa的细胞因子，通过与其受体（AMFR）相互作用的信号转导途径诱导肿瘤细胞运动，AMFR是一种78 kDa的细胞表面糖蛋白（gp78）。在先前的研究中，显示人口腔鳞状细胞癌（SCC）HOC313细胞将运动因子分泌到无蛋白质的条件培养基中。在这项研究中，我们的目的是研究AMF对人口腔鳞癌细胞的侵袭特性的作用。AMF诱导人口腔鳞癌细胞株LMF 4产生的细胞形态学改变和趋化性具有剂量和时间依赖性。AMFR mRNA的表达与SCC细胞变异体的转移能力有关。这些结果首次表明，SCC细胞产生AMF并表达AMFR，且AMFR的表达与SCC细胞的侵袭性和转移潜能有关。

Tumor cell migration is stimulated by extra cellular components, circulating serum proteins, growth and motility factors.Autocrine motility factor (AMF), a tumor-secreted 55 kDa cytokine induces tumor cell motility by a signal transduction pathway mediated by interaction with its receptor (AMFR), a cell surface glycoprotein of 78 kDa (gp78). In a previous study, it was shown that human oral sqamous cell carcinoma (SCC) HOC313 cells secrete a motility factor into a protein-free conditioned medium. In this study, we aimed to investigate the role of AMF on the invasive properties of human oral SCC cells. AMF secreted by a metastatic human oral SCC line LMF4 induced dose-and time-dependant morphological changes and chemotaxis of itself. Expression of AMFR mRNA was associated with the metastatic ability of SCC cell variants. These results show for the first time that SCC cells produce AMF and express AMFR and the expression is related to their invasiveness and metastatic potentials.