Search for invasion-and metastasis-related genes in human oral cancer

肿瘤细胞的迁移受到细胞外成分、循环血清蛋白、生长和运动因子的刺激，自分泌运动因子(AMF)是一种由肿瘤分泌的55 kDa细胞因子，通过与其受体(AMFR)(一种78 kDa的细胞表面糖蛋白)相互作用而介导的信号转导途径诱导肿瘤细胞运动。先前的研究表明，口腔鳞状细胞癌HOC313细胞在无蛋白质的条件培养液中分泌一种运动因子。在本研究中，我们旨在探讨AMF对人口腔鳞状细胞癌侵袭特性的影响。转移性人口腔鳞状细胞癌细胞系LMF4分泌的AMF诱导了剂量和时间依赖性的形态变化和自身的趋化作用。AMFR mRNA的表达与SCC细胞株的转移能力有关。这些结果首次表明SCC细胞产生AMF并表达AMFR，其表达与其侵袭和转移潜能有关。

Tumor cell migration is stimulated by extra cellular components, circulating serum proteins, growth and motility factors.Autocrine motility factor (AMF), a tumor-secreted 55 kDa cytokine induces tumor cell motility by a signal transduction pathway mediated by interaction with its receptor (AMFR), a cell surface glycoprotein of 78 kDa (gp78). In a previous study, it was shown that human oral sqamous cell carcinoma (SCC) HOC313 cells secrete a motility factor into a protein-free conditioned medium. In this study, we aimed to investigate the role of AMF on the invasive properties of human oral SCC cells. AMF secreted by a metastatic human oral SCC line LMF4 induced dose-and time-dependant morphological changes and chemotaxis of itself. Expression of AMFR mRNA was associated with the metastatic ability of SCC cell variants. These results show for the first time that SCC cells produce AMF and express AMFR and the expression is related to their invasiveness and metastatic potentials.