Genetic analysis of IM 60 common antigen from Millet cultivars (foxtail, proso, and sawa millets)

来自包含狐尾粟、proso粟和泽瓦粟的粟栽培品种的贮藏蛋白主要由醇溶性和尿素溶性级分组成，其通常分别被称为醇溶谷蛋白和谷蛋白。本研究旨在选育一个营养品质优良的谷子新品种。为此，我们首先对谷子醇溶蛋白和谷蛋白的主要组分进行了纯化和表征。结果表明：（1）3个谷子品种具有一个分子量为20 kDa的同源多肽组分，是3个谷子品种的共同抗原。(2)狐尾谷蛋白60 kDa，这是专门提取与5 M尿素，是抗原相似的60 kDa的其他品种。(3)小米60 kDa谷蛋白组分（MG 60）的N-末端氨基酸序列与水稻、大麦、玉米和小麦的淀粉结合淀粉合酶（EC www.example.com）的N-末端氨基酸序列几乎相同。使用抗MG 60抗体的Westan印迹分析表明，这些典型的谷物在谷蛋白级分中含有与MG 60相似的组分。

Storage proteins from millet cultivars containing foxtail, proso and sawa millets are mainly composed of alcohol- and urea- soluble fractions, which are usally known as prolamin and glutelin, respectively. In this studies we attempeted to develop and bringing-up an improved breed of the millet cultivars in plentifulness as well as in nutritional quality. For the purpose of them we firstly made a start on the purification and characterization of the respective major components of foxtail millet prolamin and glutelin proteins. Consequently we obtained following results :(1) the three millet cultivars share a homologous polypeptide component with a molecular weight of 20 kDa, which is a common antigen among the 3 cultivars.(2) Foxtail glutelin 60 kDa, which is exclusively extractable with 5 M urea, is antigenically similar to the 60 kDa from the other cultivars.(3) The N-terminal amino acid sequence of the millet 60 kDa glutein component (MG60) was nearly identical to that of starch-bound starch synthase (EC 2.4.1.11) of rice, barley, corn and wheat. Westan blot analysis using anti-MG60 antibodies demonstrated that these typical cereals contained a similar component to MG60 in the glutelin fractions.