Genetic analysis of IM 60 common antigen from Millet cultivars (foxtail, proso, and sawa millets)

谷子储藏蛋白主要由醇溶蛋白和尿素溶蛋白组成，通常分别称为脯蛋白和谷蛋白。在本研究中，我们试图开发和培育一个丰富和营养品质良好的谷子品种的良种。为此，我们首先对谷子中分别主要成分的脯蛋白和谷蛋白进行了纯化和鉴定。结果表明：(1)3个谷子品种具有一个分子量为20 kDa的同源多肽成分，该成分是3个谷子品种共有的抗原。(2) 60 kDa的狐尾谷蛋白与其他品种的60 kDa抗原相似，仅用5 M尿素可提取。(3)谷子60 kDa谷蛋白组分（MG60）的n端氨基酸序列与水稻、大麦、玉米和小麦的淀粉结合淀粉合成酶（EC 2.4.1.11）的n端氨基酸序列基本一致。使用抗MG60抗体的western blot分析表明，这些典型谷物在谷蛋白组分中含有与MG60相似的成分。

Storage proteins from millet cultivars containing foxtail, proso and sawa millets are mainly composed of alcohol- and urea- soluble fractions, which are usally known as prolamin and glutelin, respectively. In this studies we attempeted to develop and bringing-up an improved breed of the millet cultivars in plentifulness as well as in nutritional quality. For the purpose of them we firstly made a start on the purification and characterization of the respective major components of foxtail millet prolamin and glutelin proteins. Consequently we obtained following results :(1) the three millet cultivars share a homologous polypeptide component with a molecular weight of 20 kDa, which is a common antigen among the 3 cultivars.(2) Foxtail glutelin 60 kDa, which is exclusively extractable with 5 M urea, is antigenically similar to the 60 kDa from the other cultivars.(3) The N-terminal amino acid sequence of the millet 60 kDa glutein component (MG60) was nearly identical to that of starch-bound starch synthase (EC 2.4.1.11) of rice, barley, corn and wheat. Westan blot analysis using anti-MG60 antibodies demonstrated that these typical cereals contained a similar component to MG60 in the glutelin fractions.