Nano-Chemistry in Liquid by Photothermal Spectroscopy

我们已经开发了几种分析方法来澄清纳米空间中的液体化学现象，并在这个项目中展示了它们的有效性。在液体纳米空间的高灵敏度分子检测中，利用光热效应的热透镜显微镜被进化并优化用于实际应用。考虑到样品的表面形貌，我们对光热信号强度进行了更精确的估计，从而成功地分析了细胞上的抗原-抗体反应。此外，为了应用这种方法来监测250μm宽的通道中的络合反应，我们可以首先在玻璃芯片上集成流动注射分析。其次，我们提出了一种可以监测液/液界面分子数量的准弹性激光散射法，并对分子转移和反应进行了原位研究。在各种表面活性剂在界面上的转移、…的界面行为等方面取得了新的结果发展了飞秒脉冲激光超快透镜效应方法，用来阐明溶液中溶质到溶剂的能量传递机制和溶质-溶剂效应，研究了光激发下分子的运动和反应。在低粘度溶液中金胺O的分子动力学研究中，发现了几个PS内的快速松弛过程，发现松弛时间与溶剂的平均分子质量有关。在氨基偶氮苯的光致异构化反应中，首次测量了中间态中溶剂对驰豫过程的依赖关系，发现激光击穿等离子体中的受激拉曼散射在几十秒内就被激发出来，这是液体纳米空间中的一个新现象。所获得的拉曼光谱具有一定的特征，我们将其作为一种新的液体结构分析方法进行了研究。上述基于光热效应和毛细管波的分析方法适用于非荧光分子，其灵敏度与荧光方法相当。由此得出结论，液体中新的纳米化学世界被它们开辟得更少。

We have developed several analytical methods in order to clarify chemical phenomena in nanospace in liquid phase and shown their usefulness in this project. In highly sensitive molecular- detection in liquid nanospace, the thermal lens microscope using photothermal effects was evolved and optimized for practical applications. The photothermal signal intensity was estimated more precisely to consider the surface morphology in samples, so that we succeeded in the analysis of the antigen-antibody reactions on the cell. Further, to apply this method to monitor the chelating reaction in the 250μm width channel, we could first integrate the flow injection analysis on the glass chip. Next, we have advanced the quasi-elastic laser scattering method which can monitor the number of molecules at liquid/liquid interface, and the molecular transfer and the reaction were studied in situ. New result have been obtained on the transfer of various surfactants at the interface, the interfacial behavior o … More f phase transfer catalysis, and the hydrolysis of DPPC by a hydrolysis enzyme.Ultra fast lens effect method using femtosecond pulse laser has been developed to clarify the energy transfer mechanism from solute to solvent in liquid and the solute-solvent effect, has studied in photo-excited molecular motion and reaction. In the molecular dynamics of Auramin O in low-viscosity solutions, the fast relaxation process within several ps was found out and it was found that the relaxation time depended on the mean molecular weight of solvent. In photo isomerization of amino azobenzen, the solvent dependence on the relaxation process in the intermediated state was firstly measured.It has been founded as the novel phenomena in the liquid nanospace that the stimulated Raman scattering in laser breakdown induced plasma was emitted in the very early stage within several tens seconds. The Raman spectra obtained were characteristic and we have investigated it as the new structural analysis method in liquid.The above-mentioned analytical methods, which are based on the photothermal effect and capillary wave, are applicable for non-fluorescent molecules and their sensitivity is comparable to the fluorescent methods. It is, therefore, concluded that new nano-chemistry world in liquid is opened by them Less