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Study on effects and mechanism to oxidative capacity in skeletal muscle by physical training

体能训练对骨骼肌氧化能力的影响及机制研究

基本信息

批准号：
10308001
负责人：
HAGA Shukoh
金额：
$ 20.51万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

The molecular mechanisms mediating the cellular adaptations to exercise training in human skeletal muscle are very poorly understood. The current study was undertaken to investigate the effect of endurance training on the expression of various genes at the messenger RNA (mRNA) levels in human skeletal muscle, focusing on angiogeneic factors, antioxidant enzymes, and uncoupling proteins (UCPs), untrained male students underwent an intensive interval swimming at 80-90%V02max and 30-min long distance swimming at 70%VO2max, five times a week for 3 months. Muscle biopsies were taken before training and about 48 h after the last session. Muscle sample were obtained from the middle portion of the vastus lateralis muscle by use of the percutaneous needle biopsy technique with suction. All the subjects markedly increased their maximal oxygen uptake levels due to training (P < 0.001) using a Monark bicycle ergomater, indicating an improvement in aerobic capacity. After training there were significant (P < 0.04) decreases in the expression of mRNAs for heat shock protein 70, Cu, Zn-superoxide dismutase (Cu, Zn-SOD), and Mn-SOD but a significant (P < 0.02) increase in UCP2 mRNA expression, whereas no definite changes were observed in the levels of mRNAs for vascular endothelial growth factor (VEGF), basic fibroblast growth factor, hypoxia-inducible factor-la (HIF1a), myoglobin, or UCP3. The changes in HIF1a mRNA expression correlated well with those in VEGF mRNA expression after training (r=0.875, P < 0.01), suggesting that HIF1a influences the training-induced VEGF gene expression or alternatively that VEGF and HIE1a expressions are coregulated at the transcriptional level in human skeletal muscle. Taken together, it is envisioned that cumulative effects of transient changes in transcription during recovery from successive bouts of exercise may represent the underlying kinetic basis for the cellular adaptations associated with endurance training.

人们对调节人体骨骼肌运动训练的细胞适应的分子机制知之甚少。本研究旨在探讨耐力训练对人体骨骼肌信使RNA（mRNA）水平上各种基因表达的影响，重点关注血管生成因子、抗氧化酶和解偶联蛋白（UCP），未经训练的男学生进行了80-90%VO2max的强化间歇游泳和70%VO2max的30分钟长距离游泳，每周五次，持续 3 个月。在训练前和最后一次训练后约 48 小时进行肌肉活检。采用经皮抽吸穿刺活检技术从股外侧肌中部获取肌肉样本。由于使用 Monark 自行车测力计进行训练，所有受试者的最大摄氧量水平均显着增加 (P < 0.001)，表明有氧能力有所改善。训练后，热休克蛋白 70、Cu、Zn-超氧化物歧化酶（Cu、Zn-SOD）和 Mn-SOD 的 mRNA 表达显着降低（P < 0.04），但 UCP2 mRNA 表达显着增加（P < 0.02），而血管内皮生长因子（VEGF）、碱性成纤维细胞生长的 mRNA 水平未观察到明显变化。因子、缺氧诱导因子-1a (HIF1a)、肌红蛋白或 UCP3。训练后 HIF1a mRNA 表达的变化与 VEGF mRNA 表达的变化密切相关（r=0.875，P < 0.01），表明 HIF1a 影响训练诱导的 VEGF 基因表达，或者 VEGF 和 HIE1a 表达在人骨骼肌转录水平上受到共同调控。总而言之，可以预见的是，在连续运动恢复过程中转录瞬时变化的累积效应可能代表了与耐力训练相关的细胞适应的潜在动力学基础。