Fabrication and Application of Integrated Microchemical Systems

集成微量化学系统的制备及应用

• 批准号: 10354012
• 负责人: KITAMURA Noboru
• 金额: $ 15.87万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10354012/
• 关键词: Microchannel Chip; Polymer Substrate; Imprinting Method; MIcroelectrodes; Extraction; Microspectroscopy; Microelectrochemistry; Generation-Collection; マイクロフローセル; 顕微分光

Polymer microchannel chips were successfully fabricated on the basis of an imprinting method and were applied to in situ liquid/liquid extraction. Polymer microchannel chips with arbitraly designed microelectrodes were also developped.1). Fabrication and Application of Polymer Microchannel Chips :Polystyrol microchannel chips were fabricated by heating the polymer plate with a glass or silicon template. The channel chips were bonded with a flat polymer plate as a microfludic device. The channel chip was connected with a syringe pump through capillary tubes. The fabricated chip/fluid device was applied to liquid/liquid extraction of an aluminum chelate complex. Waterto-oil(1-butanol)extraction of the complex was followed by spatially-resolved fluorescence microscopy. The extraction efficiency in the channel chip was governed by the contact time between oil and water phases, and extraction was finished within 60 sec. The extraction efficiency was also shown to be determined by the struct … More ure of the microchannel.2). Fabrication and Characteristic Responses of Integrated Microelectrodes in Polymer Microchannel Chips :A metal layer was directly vacuum deposited on a polystyrol plate and subsequent photolithography/etching afforded microelectrodes on a polymer plate : microelectrode substrate. The microelectrode substrate was then covered with a polymer channel substrate. The electrode-channel chip was then studied by cyclic voltammetry. The electrochemical responses of the electrode in the microchannel were different from those in an ordinary microelectrode in a bulk phase. This was explained by the fact that diffusion was limited along the channel length. Generation-collection mode experiments were also conducted by using adjacent two microelectrodes in the channel. The collection efficiency in the channel was always larger than that in a bulk phase, demonstrating again characteristic electrochemical responses in a confined space. Under solution conditions, the collection efficency was as 1high as 90 %. In a bulk system, such a high efficiency is attained only by the interelectrode distance of several micrometers. Fabricated electrode-channel chips possess high potential as an microelectrochemical device. Less

聚合物微通道芯片是根据印迹方法成功制造的，并应用于原位液体/液体提取。还开发了带有仲裁设计的微电极的聚合物微通道芯片。1）。聚合物微通道芯片的制造和应用：通过用玻璃或硅模板加热聚合物板来制造聚杨醇微通道芯片。将通道芯片与扁平聚合物板粘合，作为微型浮雕装置。通道芯片与毛细管通过注射器泵连接。将制造的芯片/流体装置应用于铝制樱桃配合物的液体/液体提取。配合物的水对油（1-丁醇）提取，然后进行空间分辨的荧光显微镜。通道芯片的提取效率受油和水相之间的接触时间的控制，并在60秒内完成提取。提取效率还显示由结构……更大的微通道ure.2）。聚合物微通道芯片中集成的微电极的制造和特征反应：直接沉积在聚酯板上的金属层，并在聚合物板上提供的微电极提供的光刻型/蚀刻层，可提供微电极：微电极底物。然后用聚合物通道底物覆盖微电极底物。然后通过循环伏安法研究了电极通道芯片。微通道中电极的电化学响应与在散装相位的普通微电极中的电化学响应不同。这是通过以下事实来解释的：沿通道长度有限。还通过使用该通道中的相邻两个微电极进行了生成收集模式实验。该通道的收集效率总是比在大阶段大的收集效率更大，这再次证明了在密闭空间中的特征电化学响应。在溶液条件下，收集效率高达90％。在批量系统中，这种高效率仅通过几微米的互动距离来实现。制造的电极通道芯片作为微电化学装置的潜在高电位。较少的

项目成果

S.Ishizaka, K.Nakatani, S.Habuchi and N.Kitamura: "Total Internal Reflection Fluorescence Dynamic Anisotropy of Sulforhodamine 101 at a Liquid/Liquid Interface : Rotational Reorientation Times and Interfacial Roughness."Analytical Chemistry. 71(2). 419-42

S.Ishizaka、K.Nakatani、S.Habuchi 和 N.Kitamura：“Sulforhodamine 101 在液/液界面处的全内反射荧光动态各向异性：旋转重定向时间和界面粗糙度。”分析化学。

中谷清治: "A Study on Liquid/Liquid Extraction into Single Picoliter Droplets and in situ Electrochemical Microanalysis."Analytical Chemistry. 72(2). 339-342 (2000)

Seiji Nakatani：“单皮升液滴的液/液萃取和原位电化学微量分析”。分析化学 72(2) 339-342 (2000)。

H.-B.Kim, O.Kogi, and N.Kitamura: "Sinle-Microparticle Measurements : Laser Trapping-Absorption Microspectroscopy under Solution-Flow Conditions."Analytical Chemistry. 71(19). 4338-4343 (1999)

H.-B.Kim、O.Kogi 和 N.Kitamura：“单微粒测量：溶液流动条件下的激光捕获吸收显微光谱学”。分析化学。

金 幸夫: "Effects of Sample Dimension and Dye Distribution Characteristics in Absorption Microspectroscopy." Analytical Chemistry. 70(1). 51-57 (1998)

Yukio Kim：“吸收显微光谱中样品尺寸和染料分布特征的影响”70(1) (1998)。

八尾 浩史: "Spectroscopic and AFM Studies on the Structures of Pseudoisocyanine J Aggregates at a Mica/Water Interface"Journal of Physical Chemistry B. 103(21). 4452-4456 (1999)

Hiroshi Yao：“云母/水界面处拟异氰酸酯 J 聚集体结构的光谱和 AFM 研究”物理化学杂志 B. 103(21) (1999)。