Fabrication and Application of Integrated Microchemical Systems

集成微量化学系统的制备及应用

• 批准号: 10354012
• 负责人: KITAMURA Noboru
• 金额: $ 15.87万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10354012/
• 关键词: Microchannel Chip; Polymer Substrate; Imprinting Method; MIcroelectrodes; Extraction; Microspectroscopy; Microelectrochemistry; Generation-Collection; マイクロフローセル; 顕微分光

Polymer microchannel chips were successfully fabricated on the basis of an imprinting method and were applied to in situ liquid/liquid extraction. Polymer microchannel chips with arbitraly designed microelectrodes were also developped.1). Fabrication and Application of Polymer Microchannel Chips :Polystyrol microchannel chips were fabricated by heating the polymer plate with a glass or silicon template. The channel chips were bonded with a flat polymer plate as a microfludic device. The channel chip was connected with a syringe pump through capillary tubes. The fabricated chip/fluid device was applied to liquid/liquid extraction of an aluminum chelate complex. Waterto-oil(1-butanol)extraction of the complex was followed by spatially-resolved fluorescence microscopy. The extraction efficiency in the channel chip was governed by the contact time between oil and water phases, and extraction was finished within 60 sec. The extraction efficiency was also shown to be determined by the struct … More ure of the microchannel.2). Fabrication and Characteristic Responses of Integrated Microelectrodes in Polymer Microchannel Chips :A metal layer was directly vacuum deposited on a polystyrol plate and subsequent photolithography/etching afforded microelectrodes on a polymer plate : microelectrode substrate. The microelectrode substrate was then covered with a polymer channel substrate. The electrode-channel chip was then studied by cyclic voltammetry. The electrochemical responses of the electrode in the microchannel were different from those in an ordinary microelectrode in a bulk phase. This was explained by the fact that diffusion was limited along the channel length. Generation-collection mode experiments were also conducted by using adjacent two microelectrodes in the channel. The collection efficiency in the channel was always larger than that in a bulk phase, demonstrating again characteristic electrochemical responses in a confined space. Under solution conditions, the collection efficency was as 1high as 90 %. In a bulk system, such a high efficiency is attained only by the interelectrode distance of several micrometers. Fabricated electrode-channel chips possess high potential as an microelectrochemical device. Less

基于印迹法成功制备了聚合物微通道芯片，并将其应用于原位液/液萃取。还开发了具有任意设计的微电极的聚合物微通道芯片。聚合物微通道芯片的制作与应用：聚苯乙烯微通道芯片是通过加热聚合物板与玻璃或硅模板。将通道芯片与平坦的聚合物板结合作为微流体装置。通道芯片通过毛细管与注射泵连接。将所制造的芯片/流体装置应用于铝螯合物的液/液萃取。水-油（1-丁醇）萃取的复杂的空间分辨荧光显微镜。通道芯片中的萃取效率取决于油相和水相之间的接触时间，并且萃取在60秒内完成。萃取效率也被证明是由结构决定的。 ...更多信息 微通道的使用。2）.聚合物微通道芯片中集成微电极的制造和特性响应：将金属层直接真空沉积在聚苯乙烯板上，随后的光刻/蚀刻在聚合物板：微电极基底上提供微电极。然后用聚合物通道基底覆盖微电极基底。然后通过循环伏安法研究了电极通道芯片。电极在微通道中的电化学响应不同于普通微电极在体相中的电化学响应。这可以通过扩散沿沟道长度沿着受限的事实来解释。在通道中使用相邻的两个微电极也进行了产生-收集模式实验。在通道中的收集效率总是大于在体相中的收集效率，再次证明了在受限空间中的特征电化学响应。在溶液条件下，捕收效率高达90%。在大体积系统中，仅通过几微米的电极间距离就可获得如此高的效率。制作的电极通道芯片具有作为微电化学装置的高潜力。少

项目成果

金幸夫: "A Spatially-Resolved Fluorescence Spectroscopic Study on Liquid/Liquid Extraction Processes in Polymer Microchannels."Analytical Scienes. 16(8). 871-876 (2000)

Yukio Kane：“聚合物微通道中液/液萃取过程的空间分辨荧光光谱研究”。分析科学 16(8) (2000)。

S.Ishizaka, K.Nakatani, S.Habuchi and N.Kitamura: "Total Internal Reflection Fluorescence Dynamic Anisotropy of Sulforhodamine 101 at a Liquid/Liquid Interface : Rotational Reorientation Times and Interfacial Roughness."Analytical Chemistry. 71(2). 419-42

S.Ishizaka、K.Nakatani、S.Habuchi 和 N.Kitamura：“Sulforhodamine 101 在液/液界面处的全内反射荧光动态各向异性：旋转重定向时间和界面粗糙度。”分析化学。

中谷清治: "A Study on Liquid/Liquid Extraction into Single Picoliter Droplets and in situ Electrochemical Microanalysis."Analytical Chemistry. 72(2). 339-342 (2000)

Seiji Nakatani：“单皮升液滴的液/液萃取和原位电化学微量分析”。分析化学 72(2) 339-342 (2000)。

H.-B.Kim, O.Kogi, and N.Kitamura: "Sinle-Microparticle Measurements : Laser Trapping-Absorption Microspectroscopy under Solution-Flow Conditions."Analytical Chemistry. 71(19). 4338-4343 (1999)

H.-B.Kim、O.Kogi 和 N.Kitamura：“单微粒测量：溶液流动条件下的激光捕获吸收显微光谱学”。分析化学。

八尾 浩史: "Spectroscopic and AFM Studies on the Structures of Pseudoisocyanine J Aggregates at a Mica/Water Interface"Journal of Physical Chemistry B. 103(21). 4452-4456 (1999)

Hiroshi Yao：“云母/水界面处拟异氰酸酯 J 聚集体结构的光谱和 AFM 研究”物理化学杂志 B. 103(21) (1999)。