Fabrication and Application of Integrated Microchemical Systems

基于印迹法成功制备了聚合物微通道芯片，并将其应用于原位液/液萃取。具有任意设计微电极的聚合物微通道芯片也被开发出来。聚合物微通道芯片的制备与应用：采用玻璃或硅模板加热聚合物板制备聚苯乙烯微通道芯片。通道芯片与平面聚合物板结合作为微流控装置。通道芯片通过毛细管与注射泵连接。将自制的芯片/流体装置应用于铝螯合物的液/液萃取。利用空间分辨荧光显微镜对配合物进行水-油（1-丁醇）萃取。通道芯片的萃取效率受油相与水相接触时间的影响，萃取可在60秒内完成，萃取效率也受微通道结构的影响(2)。聚合物微通道芯片中集成微电极的制备和特性响应：将金属层直接真空沉积在聚苯乙烯板上，随后光刻/蚀刻在聚合物板上形成微电极：微电极衬底。然后用聚合物通道衬底覆盖微电极衬底。然后用循环伏安法对电极-通道芯片进行了研究。电极在微通道中的电化学响应与普通微电极在体相中的电化学响应不同。这可以用沿通道长度扩散受到限制这一事实来解释。利用通道内相邻的两个微电极进行了生成-收集模式实验。通道中的收集效率总是大于体相中的收集效率，再次证明了在密闭空间中的特征电化学响应。在溶液条件下，收集效率高达90%。在块体系统中，只有电极间距离达到几微米才能达到如此高的效率。制备的电极通道芯片作为一种微电子化器件，具有很高的发展潜力。少

Polymer microchannel chips were successfully fabricated on the basis of an imprinting method and were applied to in situ liquid/liquid extraction. Polymer microchannel chips with arbitraly designed microelectrodes were also developped.1). Fabrication and Application of Polymer Microchannel Chips :Polystyrol microchannel chips were fabricated by heating the polymer plate with a glass or silicon template. The channel chips were bonded with a flat polymer plate as a microfludic device. The channel chip was connected with a syringe pump through capillary tubes. The fabricated chip/fluid device was applied to liquid/liquid extraction of an aluminum chelate complex. Waterto-oil(1-butanol)extraction of the complex was followed by spatially-resolved fluorescence microscopy. The extraction efficiency in the channel chip was governed by the contact time between oil and water phases, and extraction was finished within 60 sec. The extraction efficiency was also shown to be determined by the struct … More ure of the microchannel.2). Fabrication and Characteristic Responses of Integrated Microelectrodes in Polymer Microchannel Chips :A metal layer was directly vacuum deposited on a polystyrol plate and subsequent photolithography/etching afforded microelectrodes on a polymer plate : microelectrode substrate. The microelectrode substrate was then covered with a polymer channel substrate. The electrode-channel chip was then studied by cyclic voltammetry. The electrochemical responses of the electrode in the microchannel were different from those in an ordinary microelectrode in a bulk phase. This was explained by the fact that diffusion was limited along the channel length. Generation-collection mode experiments were also conducted by using adjacent two microelectrodes in the channel. The collection efficiency in the channel was always larger than that in a bulk phase, demonstrating again characteristic electrochemical responses in a confined space. Under solution conditions, the collection efficency was as 1high as 90 %. In a bulk system, such a high efficiency is attained only by the interelectrode distance of several micrometers. Fabricated electrode-channel chips possess high potential as an microelectrochemical device. Less