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Novel Trace Analysis Systems Based on Single Picoliter Microparticles Combined with Microflow Devices

基于单皮升微粒与微流装置相结合的新型痕量分析系统

基本信息

批准号：
19205010
负责人：
KITAMURA Noboru
金额：
$ 32.2万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

In the present study, we showed that a single 1-butanol (BuOH) droplet could be produced by infrared laser irradiation under an optical microscope and the droplet produced could be simultaneously trapped optically by the infrared laser beam. The technique was then combined with a capillary electrophoresis system. Single BuOH droplets were produced by laser irradiation and trapped stably even in the presence of an osmotic flow. An aluminum dihydroxyazobenzene chelate complex (Al^<3+>-DHAB) or rhodamine B (RhB) was then separated by capillary electrophoresis and the solute was then forced to distribute to single BuOH droplets produced by infrared laser irradiation. In the case of RhB, the lower detection limit of the dye distributed to the BuOH droplet was as low as 10^<-20>mol/dm^3, which was much lower than the ordinary detection limits in capillary electrophoresis.Laser oscillation in single dye-doped cation-exchange resin microspheres was also applied to ultratrace analysis. Laser oscillation of single RhB-doped microparticles under air was confirmed by characteristic emission spikes superimposed to the spontaneous broad emission spectrum. In the presence of organic gas vapor in air, the characteristic laser oscillation spikes from the RhB-doped microparticle were modulated owing to the change in the particle/surrounding medium refractive index conditions. The present study was also extended to laser trapping-microspectroscopy of picoliter aerosol water droplets in air. We succeeded in conducting temperature-controlled laser trapping of aerosol water droplets as well as in observing formation of supercooled water droplets down to 223 K.

在本研究中，我们证明了在光学显微镜下，红外激光照射可以产生单一的正丁醇(BuOH)液滴，并且产生的液滴可以同时被红外激光光学捕获。然后将这项技术与毛细管电泳仪相结合。单一的丁醇液滴是由激光照射产生的，即使在渗透流存在的情况下也能稳定地捕获。然后用毛细管电泳法分离二羟基偶氮苯铝络合物(Al^&lt；3+&gt；-DHAB)或罗丹明B(RhB)，然后迫使溶质分散到红外激光照射产生的单个丁醇液滴中。在RhB的情况下，染料分布在BuOH液滴中的检测下限低至10~(-20)mol/dm~(-3)，远低于毛细管电泳法的常规检测下限。单个RhB掺杂微粒子在空气中的激光振荡是由叠加在自发宽发射光谱上的特征发射峰证实的。在空气中有机气体存在的情况下，由于颗粒/周围介质折射率条件的改变，掺杂RhB的微粒产生的特征激光振荡尖峰被调制。本研究还扩展到空气中皮升气溶胶水滴的激光捕获显微光谱分析。我们成功地进行了气溶胶水滴的温度控制激光捕获，并观察到了低至223K的过冷水滴的形成。