Novel Trace Analysis Systems Based on Single Picoliter Microparticles Combined with Microflow Devices

基于单皮升微粒与微流装置相结合的新型痕量分析系统

• 批准号: 19205010
• 负责人: KITAMURA Noboru
• 金额: $ 32.2万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19205010/
• 关键词: 単一微粒子; 高感度分析; レーザー捕捉; レーザー発振; マイクロチップ

In the present study, we showed that a single 1-butanol (BuOH) droplet could be produced by infrared laser irradiation under an optical microscope and the droplet produced could be simultaneously trapped optically by the infrared laser beam. The technique was then combined with a capillary electrophoresis system. Single BuOH droplets were produced by laser irradiation and trapped stably even in the presence of an osmotic flow. An aluminum dihydroxyazobenzene chelate complex (Al^<3+>-DHAB) or rhodamine B (RhB) was then separated by capillary electrophoresis and the solute was then forced to distribute to single BuOH droplets produced by infrared laser irradiation. In the case of RhB, the lower detection limit of the dye distributed to the BuOH droplet was as low as 10^<-20>mol/dm^3, which was much lower than the ordinary detection limits in capillary electrophoresis.Laser oscillation in single dye-doped cation-exchange resin microspheres was also applied to ultratrace analysis. Laser oscillation of single RhB-doped microparticles under air was confirmed by characteristic emission spikes superimposed to the spontaneous broad emission spectrum. In the presence of organic gas vapor in air, the characteristic laser oscillation spikes from the RhB-doped microparticle were modulated owing to the change in the particle/surrounding medium refractive index conditions. The present study was also extended to laser trapping-microspectroscopy of picoliter aerosol water droplets in air. We succeeded in conducting temperature-controlled laser trapping of aerosol water droplets as well as in observing formation of supercooled water droplets down to 223 K.

在本研究中，我们表明，在光学显微镜下红外激光照射可以产生单个1-丁醇（BuOH）液滴，并且可以同时被红外激光束在光学上同时将产生的液滴捕获。然后将该技术与毛细管电泳系统结合使用。通过激光照射产生单个BuOH液滴，即使在存在渗透流动的情况下，也稳定地被困。然后通过毛细管电泳将铝二羟基苯苯基苯螯合络合物（Al^<3+> - dhab）或若丹明B（RHB）分离，然后将溶质迫使溶质分发给由红外激光辐射产生的单个BuoH液滴。在RHB的情况下，分布在BuOH液滴的染料的较低检测极限低至10^<-20> mol/dm^3，远低于毛细管电泳中的普通检测限。在单个染料掺杂的阳离子阳离子 - exchange-exchange-exchange-exchange-exchange-exchange-excleres中也将其振荡应用于Ultoratrace分析。空气下单个RHB掺杂的微粒的激光振荡通过叠加到自发的宽发射光谱的特征发射尖峰证实。在空气中存在有机气蒸气的情况下，由于粒子/周围培养基折射率条件的变化，调节了来自RHB掺杂的微粒的特征激光振荡尖峰。本研究还扩展到了空气中的Picoliter气溶胶水滴的激光诱捕微光谱。我们成功地进行了气溶胶水滴的温度控制激光捕获，并观察到超冷水滴的形成至223 k。

项目成果

Phase separation dynamics of aqueous solutions of thermoresponsive polymers studied by a laser T-jump technique.

• DOI: 10.1021/jp711128s
• 发表时间: 2008-02
• 期刊: The journal of physical chemistry. B
• 作者: Yasuyuki Tsuboi;Y. Yoshida;K. Okada;N. Kitamura

A laser trapping and spectroscopy study on photoinduced water droplets formation in air

空气中光致水滴形成的激光捕获和光谱研究

• 发表时间: 2011
• 作者: Kian Ping Gan;Masafumi Yoshio;Takashi Kato;Shoji Ishizaka
• 通讯作者: Shoji Ishizaka

レーザー捕捉・顕微ラマン分光を用いた過冷却微小水滴の相転移温度の決定

使用激光捕获和显微拉曼光谱测定过冷微水滴的相变温度

• 发表时间: 2008
• 作者: 和田照秀;石坂昌司;喜多村昇
• 通讯作者: 喜多村昇

極限分析

极限分析

• 发表时间: 2008
• 作者: Yoshiyuki Hatakeyama;Tomohisa Sawada;Masaki Kawano;Makoto Fujita;三浦均;U.G.Gautam;T. Tahara;S. Maekawa;松田欣之;篠原久典;喜多村昇
• 通讯作者: 喜多村昇

化学フロンテイア : 切り拓き、サイエンスを牽引する分析最前線

化学前沿：开拓和推动科学的前沿分析

• 发表时间: 2008
• 作者: P. Sen;S. Yamaguchi;T. Tahara;酒井大地;A.Rana;喜多村〓(分担)
• 通讯作者: 喜多村〓(分担)