The diversified research on. the genes related periodontal disease

在本项目中，我们从感染细菌、宿主防御系统、组织代谢和组织再生等方面对牙周病相关基因进行了综述。通过对牙龈卟啉单胞菌血凝素DNA序列的分析，揭示了其功能结构域。结合结构域包含与流感病毒类似的特定序列PVQNT。从伴放线放线杆菌中克隆了一个编码细胞致死性膨胀毒素(CDT)的新基因(AA CDT)。AA CDT由三个簇组成，每个簇编码CDT-A、-B和-C。CDT-A、-B和-C是表达CDT活性所必需的。用T细胞受体(TCR)和人类白细胞抗原(HLA)检测牙周炎患者自身抗体的产生。外周T细胞用hrHSP60或牙龈假单胞菌GroEL及其TCRCDR3区β链DNA序列刺激。扩增的T细胞…与CDR3的氨基酸序列一致炎症牙龈组织中克隆较多，T细胞克隆较多。应用聚合酶链式反应-限制性片段长度多态性方法分析牙周炎、自身抗体产生和PVB19感染患者的人类白细胞抗原II类基因分型。DQA1*0101、DQA1*0501和DQB1*0503在牙周炎、自身抗体产生和PVB19感染患者中的频率高于其他患者和健康对照组。IL-1基因与成人牙周炎患病率的关系在美国已有报道，但在日本尚未发现这种关系。对两例常合并牙周炎的低磷患者及其家族成员进行了组织非特异性碱性磷酸酶基因突变检测。发现了碱性磷酸酶基因的三个新的点突变。通过组织修复中的创伤牙周组织和健康牙周组织的消减方法，发现了与牙周组织再生有关的新基因。成功克隆了16个新的增强表达基因和9个新的下调表达基因。较少

In this project, we evaluate the genes related periodontal disease from the diversified aspects, infected bacteria, host defense system, tissue metabolism, and tissue regeneration. Analysis of DNA sequence of hemagglutinin from Porphyromonas gingivalis revealed its functional domain. The binding domain contains the specific sequence, PVQNT, similar to influenza virus. A novel gene (Aa cdt) encoding cytolethal distending toxin (CDT) was successfully cloned from Actinobacillus actinomycetemcomitans. Aa cdt was composed with three clusters, and each cluster was encoding CDT-A, -B, and -C. CDT-A, -B and -C were essential for expressing CDT activity.The genes related autoantibody production in patients with periodontitis were evaluated on T cell receptor (TCR) and human leukocyte antigen (HLA). The peripheral T cells were stimulated by hrHSP60 or P. gingivalis GroEL and DNA sequence of CDR3 region of their TCR β-chain. The amino-acid sequenee of CDR3 was the same between the expanded T cell … More clone and the accumulated T cell clone in inflamed gingival tissue. The HLA class II genotypes of patients with periodontitis, autoantibody production and PVB 19 infection were analyzed by PCR-RFLP. The frequencies of DQA1 *0101, DQA1 *0501, and DQB1*0503 in patients with periodontitis, autoantibody production and PVB 19 infection were higher than in other patients and healthy subjects. The relationship between IL-1 genotypes and prevalence of adult periodontitis was reported in the United State, however we could not found this relationship in Japanese.Two patients with hypophosphatasia, usually accompanied with periodontitis, and their family members were analyzed to detect mutation on tissue-nonspecifie alkaline phosphatase gene. Novel three point mutations on the alkaline phosphatase gene were revealedNovel genes related periodontal tissue regeneration were found by the subtraction method between wound periodontal tissue under tissue repair and healthy periodontal tissue. Sixteen novel cDNAS in enhanced expression genes and 9 novel cDNAS in depressed expression genes were successfully cloned. Less