The auto-immune mechanisms on the development of earl -onset periodontitis

在这个项目中，我们评估自身免疫机制在早发性牙周炎的发展。首先，分析了牙周炎患者牙龈成纤维细胞（GF）和牙周韧带源性成纤维细胞（PLF）自身抗体的存在情况。采用免疫印迹法和酶联免疫吸附法对51例牙周炎患者和12例健康人进行了检测。Western blotting分析发现，早发性牙周炎（EOP）患者常在GF中识别自身抗原。尤其在EOP患者血清IgG中，95 kDa和108 kDa抗原被清晰地检测到。这两种抗原也能被免疫直弯曲杆菌的兔血清IgG识别。具有这些自身抗体的患者血清中抗直型C的IgG滴度较高。因此，GF抗原与直梭菌抗原之间可能存在分子模仿性和交叉反应性。一些病毒感染与自身免疫性疾病有关，如类风湿关节炎。采用ELISA法分析细小病毒b19 （PVB 19）感染与自身抗体产生的关系。牙周炎患者PVB - 19感染率高于健康人群。所有对GF或PLF产生高水平自身抗体的患者均感染PVB19。免疫反应是由主要的组织相容性复合体（MHC，人白细胞抗原（HLA））所抑制的。然后，我们进一步分析了牙周炎患者的HLA 11类基因型、自身抗体产生和PVB 19感染。采用PCR-RFLP分析确定HLAⅱ类基因型。DQA1*0101、DQA1*0501和DQB1*0503在牙周炎、自身抗体产生和PVB 19感染患者中的频率高于其他患者和健康者。

In this project, we evaluate autoimmune mechanisms on the development of early-onset periodontitis. Firstly, the presence of autoantibodies against human gingival fibroblast (GF) and human periodontal ligament derived fibroblast (PLF) in patients with periodontitis was analyzed. Totally fifty-one patients with periodontitis and 12 healthy subjects were examined with Western blotting and ELISA. On Western blotting analyses, patients with early-onset periodontitis (EOP) frequently recognized auto-antigen in GF. Especially, antigens with 95 kDa and 108 kDa were clearly detected with serum IgG from EOP patients. Both antigens were also recognized with serum IgG from rabbits immunized Campylobactor rectus. Patients who have these autoantibodies showed high serum IgG titer against C rectus. Therefore, there may be molecular mimicry and cross reactivity between GF antigen and C. rectus antigen. Some viral infections were involved in autoirnmune disease, such as Rheumatoid arthritis. Then, the relationship between parvovirus B 19 (PVB 19) infection and autoantibody production was analyzed by ELISA. The ratio of PVB 19 infection in patients with periodontitis was higher than in healthy subjects. All patients who produce high level of autoanubody against GF or PLF were infected with PVB19. Immune response is known to be resincted by the major histocompatible complex (MHC, human leukocyte antigen (HLA) in human). Then, we further analyzed the HLA class 11 genotypes of patients with periodontitis, autoantibody production and PVB 19 infection. PCR-RFLP analyses were taken to detenuine HLA class II genotype. The frequencies of DQA1*0101, DQA1*0501, and DQB1*0503 in patients with periodontitis, autoantibody production and PVB 19 infection were higher in other patients and healthy subjects.