The auto-immune mechanisms on the development of earl -onset periodontitis

在这个项目中，我们评估了自身免疫机制在早发性牙周炎发生中的作用。首先，分析了牙周炎患者牙周组织中抗人牙龈成纤维细胞(GF)和人牙周膜成纤维细胞(PLF)自身抗体的存在情况。对51例牙周炎患者和12例健康体检者进行免疫印迹和酶联免疫吸附试验。在Western blotting分析中，早发性牙周炎(EOP)患者经常识别GF中的自身抗原。特别是，EOP患者血清中可清晰检测到95 kDa和108 kDa抗原。这两种抗原也可与免疫的兔直肠弯曲菌血清中的抗体结合。具有这些自身抗体的患者血清中C-直肌抗体滴度较高。因此，GF抗原与直肠肌抗原之间可能存在分子模拟和交叉反应。一些病毒感染与自身免疫性疾病有关，如类风湿性关节炎。用酶联免疫吸附试验分析细小病毒B19(PVB19)感染与自身抗体产生的关系。牙周炎患者的PVB19感染率明显高于正常对照组。所有产生高水平抗GF或PLF自身抗体的患者均感染PVB19。已知的免疫反应是由主要组织相容复合体(MHC，人类白细胞抗原)激活的。然后，进一步分析牙周炎患者的HLA11基因分型、自身抗体产生情况和PVB19感染情况。采用聚合酶链式反应-限制性片段长度多态性方法检测人类白细胞抗原II类基因。DQA1*0101、DQA1*0501和DQB1*0503在牙周炎、自身抗体产生和PVB19感染患者中的频率较高，在其他患者和正常人中较高。

In this project, we evaluate autoimmune mechanisms on the development of early-onset periodontitis. Firstly, the presence of autoantibodies against human gingival fibroblast (GF) and human periodontal ligament derived fibroblast (PLF) in patients with periodontitis was analyzed. Totally fifty-one patients with periodontitis and 12 healthy subjects were examined with Western blotting and ELISA. On Western blotting analyses, patients with early-onset periodontitis (EOP) frequently recognized auto-antigen in GF. Especially, antigens with 95 kDa and 108 kDa were clearly detected with serum IgG from EOP patients. Both antigens were also recognized with serum IgG from rabbits immunized Campylobactor rectus. Patients who have these autoantibodies showed high serum IgG titer against C rectus. Therefore, there may be molecular mimicry and cross reactivity between GF antigen and C. rectus antigen. Some viral infections were involved in autoirnmune disease, such as Rheumatoid arthritis. Then, the relationship between parvovirus B 19 (PVB 19) infection and autoantibody production was analyzed by ELISA. The ratio of PVB 19 infection in patients with periodontitis was higher than in healthy subjects. All patients who produce high level of autoanubody against GF or PLF were infected with PVB19. Immune response is known to be resincted by the major histocompatible complex (MHC, human leukocyte antigen (HLA) in human). Then, we further analyzed the HLA class 11 genotypes of patients with periodontitis, autoantibody production and PVB 19 infection. PCR-RFLP analyses were taken to detenuine HLA class II genotype. The frequencies of DQA1*0101, DQA1*0501, and DQB1*0503 in patients with periodontitis, autoantibody production and PVB 19 infection were higher in other patients and healthy subjects.