The auto-immune mechanisms on the development of earl -onset periodontitis

早发性牙周炎发生发展的自身免疫机制

• 批准号: 10470459
• 负责人: KURIHARA Hidemi
• 金额: $ 4.86万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470459/
• 关键词: autoantibody; human gingival fibroblast; parvovirus B19; Campylobactor rectus; HLA class 11; genotyping; 早期発症型歯周炎; 自己抗原; Th1; Th2; Parvovirus B19; Campylobacter reclus

In this project, we evaluate autoimmune mechanisms on the development of early-onset periodontitis. Firstly, the presence of autoantibodies against human gingival fibroblast (GF) and human periodontal ligament derived fibroblast (PLF) in patients with periodontitis was analyzed. Totally fifty-one patients with periodontitis and 12 healthy subjects were examined with Western blotting and ELISA. On Western blotting analyses, patients with early-onset periodontitis (EOP) frequently recognized auto-antigen in GF. Especially, antigens with 95 kDa and 108 kDa were clearly detected with serum IgG from EOP patients. Both antigens were also recognized with serum IgG from rabbits immunized Campylobactor rectus. Patients who have these autoantibodies showed high serum IgG titer against C rectus. Therefore, there may be molecular mimicry and cross reactivity between GF antigen and C. rectus antigen. Some viral infections were involved in autoirnmune disease, such as Rheumatoid arthritis. Then, the relationship between parvovirus B 19 (PVB 19) infection and autoantibody production was analyzed by ELISA. The ratio of PVB 19 infection in patients with periodontitis was higher than in healthy subjects. All patients who produce high level of autoanubody against GF or PLF were infected with PVB19. Immune response is known to be resincted by the major histocompatible complex (MHC, human leukocyte antigen (HLA) in human). Then, we further analyzed the HLA class 11 genotypes of patients with periodontitis, autoantibody production and PVB 19 infection. PCR-RFLP analyses were taken to detenuine HLA class II genotype. The frequencies of DQA1*0101, DQA1*0501, and DQB1*0503 in patients with periodontitis, autoantibody production and PVB 19 infection were higher in other patients and healthy subjects.

在这个项目中，我们评估自身免疫机制对早发性牙周炎的发展。首先，分析了牙周炎患者中抗人牙龈成纤维细胞（GF）和人牙周膜衍生成纤维细胞（PLF）自身抗体的存在。采用Western blotting和ELISA法检测51例牙周炎患者和12例健康对照者的牙周膜蛋白。在Western印迹分析中，早发性牙周炎（EOP）患者经常识别GF中的自身抗原。特别是95 kDa和108 kDa的抗原与EOP患者血清中的IgG明确检测。这两种抗原也被弯曲杆菌免疫的兔血清IgG识别。具有这些自身抗体的患者显示出针对直肌的高血清IgG滴度。因此，GF抗原与C.腹直肌抗原一些病毒感染参与了自身免疫性疾病，如风湿性关节炎。用ELISA法分析细小病毒B 19（PVB 19）感染与自身抗体产生的关系.牙周炎患者中PVB 19感染率高于健康受试者。所有产生高水平抗GF或PLF自身抗体的患者均感染了PVB 19。已知免疫应答由主要组织相容性复合体（MHC，人类白细胞抗原（HLA））抑制。进一步分析了牙周炎、自身抗体产生和PVB 19感染患者的HLA 11基因型。采用PCR-RFLP方法检测HLA Ⅱ类基因型。DQA 1 0 10 1、DQA 1 0 5 0 1和DQB 1 0 5 0 3在牙周炎患者中的频率、自身抗体产生和PVB 19感染在其他患者和健康受试者中的频率较高。

项目成果

Okada,M.,et al.: "Genetic,Immunological and Microbiolobical Study of Family Members Manifesting Early-Onset Periodontitis"J.Dent.Res.. 78. 513-513 (1999)

Okada,M.,et al.：“表现早发性牙周炎的家庭成员的遗传、免疫学和微生物学研究”J.Dent.Res.. 78. 513-513 (1999)

Yoshino,H,et al.: "Autoantibody against gingival fibroblast antigen in serum from patients with periodontitis" J.Dent.Res.77. 648-648 (1998)

Yoshino,H,et al.：“牙周炎患者血清中抗牙龈成纤维细胞抗原的自身抗体”J.Dent.Res.77。

Okada,M.,el.al.: "Genetic,Immunological and Microbiolobical Study of Family Members Manifesting EarlyーOnset Periodontitis"J.Dent.Res.. 78. 513-513 (1999)

Okada, M., el.al.：“表现早发性牙周炎的家庭成员的遗传、免疫学和微生物学研究”J.Dent.Res.. 78. 513-513 (1999)

Okada,M.,et al.: "Genetic,Immunological and Microbiolobical Study of Family Members Manifesting Early-Onset Periodontitis" J.Dent.Res.78. 513-513 (1999)

Okada,M.,et al.：“表现早发性牙周炎的家庭成员的遗传、免疫学和微生物学研究”J.Dent.Res.78。

Okada, M., et al.: "Genetic, immunological and microbiological study of family members manifesting early-onset Periodontitis"Journal of Dental Research. 78. 513-513 (1999)

Okada, M., 等人：“对表现出早发性牙周炎的家庭成员的遗传、免疫学和微生物学研究”《牙科研究杂志》。