Periodontal tissue regeneration with autologous cell transplantation of bone marrow mesenchymal stem cells in dogs

犬自体骨髓间充质干细胞移植牙周组织再生

• 批准号: 13470464
• 负责人: KURIHARA Hidemi
• 金额: $ 9.02万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470464/
• 关键词: mesenchymal stem cell; pluripotent; regenerative therapy; experimental periodontal defect; periodontal regeneration; collagen gel; tissue engineering; bone marrow; 多分化能; 歯周組織再生療法; コラーゲンゲル; 骨髄液

Some studies have shown that mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells are useful for autologous cell transplantation. In this study, we examined usefulness for MSC transplantation into the periodontal defect. Bone marrow MSC were isolated from beagle dogs and expanded in vitro. The expanded MSC were mixed with 2% atelocollagen in various concentration (0, 2x10^6, 5x10^6, 1x10^7, 2x10^7 cells/ml) and auto-transplanted to experimental class III defects. Periodontal tissue healing was evaluated both histological and morphometrical analyses at one month after transplantation. The defects were almost completely regenerated with alveolar bone, periodontal ligament and cementum in 2x10^7 cells/ml group. Morphometrical analysis revealed that newly formed bone and cementum rates in the 2x10^7 cells/ml group was significantly higher than that in 0 cells/ml group (p<0.01). These results indicate that the auto-transplantation of bone marrow MSC might become a useful option for periodontal tissue regeneration.

一些研究表明，间充质干细胞(MSC)可以分化为各种结缔组织细胞，可用于自体细胞移植。在这项研究中，我们研究了骨髓间充质干细胞移植到牙周缺损处的有用性。从Beagle犬分离骨髓MSC并进行体外扩增。将扩增的MSC以不同浓度(0、2×10~(-6)、5×10~(-6)、1×10~(-7)、2×10~(-7)细胞/毫升)与2%胶原混合，自体移植至实验III类缺损处。移植后1个月进行组织学和形态计量学分析，评价牙周组织愈合情况。2×10~(-7)个/ml组的牙槽骨、牙周膜和牙骨质几乎完全再生。形态计量学分析显示，2×107个/ml组新生骨和牙骨质形成率明显高于0个/ml组(P<0.01)。提示自体骨髓间充质干细胞移植有望成为牙周组织再生的有效选择。

项目成果

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