Studies on the roles of auto-antibodies on the development of periodontal disease

自身抗体在牙周病发生发展中作用的研究

• 批准号: 07457456
• 负责人: KURIHARA Hidemi
• 金额: $ 4.54万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457456/
• 关键词: auto-antibody; common antigen; human gingival fibroblast; periodontopathic bacteria; collagen; IgGサブクラス; 歯周病; 自己抗原

In this project, we analyzed the presence of auto-antibodies against human gingival fibroblast (HGF) in patients with periodontitis. One hundred patients with periodontitis (56 patients with adult periodontitis, 44 patients with early-onset periodontitis) and 12 healthy subjects were examined by Western blotting. Fifty percent of patients with adult periodontitis, 51.2% of patients with early-onset periodontitis and 16.7% of healthy subjects possessed the serum IgG antibody sgainst HGF.There was no difference on the presence of auto-antibody and the variety of antigens among various types of periodontal diseases. There was no relationship between HLA class II geno-type of the patients and the HGF antigens recognized by the patients. The HGF antigens recognized by the patient serum were one band to several bands. The responses were varied among patients. In these antigens, the antigen with 180 kDa was strongly recognized by the many patients. The characters of this antigen were follows. 1)The molecular weight of this antigen was higher than 180 kDa under non reduced condition. 2)This antigen was commonly present in human periodonal ligament fibroblast and KB cell. 3)The molecular weight of this antigen was changed to 110 kDa after collagenese treatment. 4)The antibodies against human type I collagen and type II collagen were not reacted to this antigen. The common antigenicity between periodontopathic bacteria antigen and HGF antigenen was determined with the derum from the rabbits immunized with 17 periodontopathic bacteria. The HGF antigen with 55 kDa was recognized by various rabbit antiserum. The anti-Campylobacter rectus rabbit serum was strongly reacted with the HGF antigen with 35 kDa. These antigens with 55 kDa and 35 kDa had the same molecular weight to the antigens recognized with the patient serum. There may be the common epitopes between HGF antigens and periodontopathic bacteria antigens.

在这个项目中，我们分析了牙周炎患者中抗人牙龈成纤维细胞（HGF）自身抗体的存在。采用Western blotting方法检测100例牙周炎患者（成人牙周炎56例，早发性牙周炎44例）和12例健康对照者的牙周组织。50%的成人牙周炎患者、51.2%的早发性牙周炎患者和16.7%的健康人血清IgG抗体阳性，不同类型牙周病患者血清IgG抗体阳性率和抗原种类无明显差异。HLA-II类基因型与患者识别的HGF抗原无关。患者血清识别的HGF抗原为一条带至多条带。患者的反应各不相同。在这些抗原中，180 kDa的抗原被许多患者强烈识别。该抗原的特性如下。1)该抗原在非还原条件下的分子量大于180 kDa。2)该抗原普遍存在于人牙周膜成纤维细胞和KB细胞中。3)经胶原酶处理后，该抗原的分子量变为110 kDa。4)抗人I型胶原和II型胶原的抗体不与该抗原反应。用17种牙周致病菌免疫家兔，取血清测定牙周致病菌抗原与肝细胞生长因子抗原的共同抗原性。HGF抗原分子量为55 kDa，能被多种兔抗血清识别。抗弯曲菌兔血清与35 kDa的HGF抗原呈强反应。这些具有55 kDa和35 kDa的抗原具有与患者血清识别的抗原相同的分子量。HGF抗原与牙周致病菌抗原之间可能存在共同的表位。

项目成果

Hino, T.: "Antibody to L-arginine-sensitive hemagglutinin from Fusobacterium nucleatum in periodontitis patients." J Dent Res. 76(Special Issue). 410 (1997)

Hino, T.：“牙周炎患者中具核梭杆菌的 L-精氨酸敏感血凝素抗体。”

Takahashi, K.: "Assessment of in vitro interleukin-2-producing capacity of peripheral blood lymphocytes from patients with periodontitis" J Clin Periodontol. 24. 44-50 (1997)

Takahashi, K.：“牙周炎患者外周血淋巴细胞体外产生白细胞介素 2 的能力的评估”J Clin periodontol。

Ninomiya, J.: "Cellular immuno-competence of infected root canal contents in pathogenesis of periapical lesion." J Endodontics. (in press).

Ninomiya, J.：“感染根管内容物的细胞免疫能力在根尖周病变发病机制中的作用。”

河野隆幸ら: "歯周病患者が認識する歯肉線維芽細胞由来高分子蛋白の検出" 日本歯科保存学会誌. 39(春季特別号). 5 (1996)

Takayuki Kono 等：“牙周病患者识别的牙龈成纤维细胞衍生的高分子量蛋白质的检测”日本保守牙科学会杂志 39（春季特刊）5（1996 年）。

Arai, H.: "Host deffensive function in a family manifesting early-onset periodontitis." J Periodontol. 67. 433-442 (1996)

Arai, H.：“表现出早发性牙周炎的家庭中的宿主防御功能。”