Inhibitory effects of sugar alcohols on acid production of dental plaque bacteria

糖醇对牙菌斑细菌产酸的抑制作用

• 批准号: 10470387
• 负责人: YAMADA Tadashi
• 金额: $ 6.66万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470387/
• 关键词: Streptococcus mutans; sugar metabolism; metabolism of sugar alcohols; sorbitol; xylitol; oxygen; inhibition of metabolism

1. The aim of this study was to examine the effect of temporary exposure of the streptococcal cells to air on the intracellular flux of glucose and sorbitol metabolism by measuring acid excretion, fluorescence dependent on cellular level of NADH, glycolytic intermediates and enzyme activities. The exposure of cells to air decreased the acid excretions during glucose and sorbitol metabolism. The analysis of the glycolytic intermediates and the fluorescence suggested that the reduced level of acid excretion in the air-exposed glucose metabolizing cells resulted from the decrease in pyruvate catabolism. In the presence of sorbitol, the decreased acid production resulted from the reduced rates of the reactions catalyzed by sorbitol-phosphoenolpyruvate phosphotransferase and sorbitol 6-phosphate dehydrogenase because of shortage of substrates for these enzymes in addition to the decrease in pyruvate catabolism.2. The aim of this study was to examine glucose and sorbitol metabolism in Strept … More ococcus mutans cells exposed and not exposed to air at the coexistence of these compounds by measuring acid excretion, levels of fluorescence dependent on cellular NADH and glycolytic intermediates. A part of bacterial cells grown under strictly anaerobic conditions (anaerobic cells) was exposed temporarily to air (aerobic cells). When glucose was added to the anaerobic cells metabolizing sorbitol, the acid excretion was increased. At the same time, the level of NADH was decreased and then reached to the higher plateau level than that during glucose metabolism. The aerobic cells neither metabolized sorbitol nor contained cellular intermediates. At 2 min after glucose was added in the presence of sorbitol, the acid excretion was started slowly and the intermediates appeared. The level of NADH was decreased at first and then increased. These results suggested that the anaerobic S. mutans cells metabolized glucose and sorbitol simultaneously, and that in the presence of sorbitol the aerobic cells could start to metabolize glucose by addition of glucose at 2 min after glucose was added, as the intermediates (PEP potential) for the glucose transport were accumulated. Less

1.本研究的目的是检查的链球菌细胞暂时暴露于空气中的葡萄糖和山梨醇代谢的细胞内通量的影响，通过测量酸排泄，荧光依赖于细胞水平的NADH，糖酵解中间体和酶活性。暴露于空气中的细胞减少葡萄糖和山梨醇代谢过程中的酸分泌。糖酵解中间产物和荧光分析表明，暴露于空气中的葡萄糖代谢细胞中酸排泄水平的降低是由于丙酮酸催化剂的减少。在山梨醇存在下，产酸量的降低是由于丙酮酸分解代谢的减少以及底物的缺乏，导致山梨醇-磷酸烯醇丙酮酸磷酸转移酶和山梨醇6-磷酸脱氢酶催化的反应速率降低.本研究的目的是检查葡萄糖和山梨醇代谢的Strept ...更多信息 通过测量酸排泄、依赖于细胞NADH和糖酵解中间体的荧光水平，观察暴露于和未暴露于空气中的变形球菌细胞在这些化合物共存时的变化。一部分在严格厌氧条件下生长的细菌细胞（厌氧细胞）暂时暴露于空气（需氧细胞）。当向代谢山梨醇的厌氧细胞中加入葡萄糖时，酸排泄增加。与此同时，还原型辅酶Ⅱ（NADH）水平下降，并达到高于糖代谢的平台水平。好氧细胞既不代谢山梨醇，也不含有细胞中间产物。在山梨糖醇存在下加入葡萄糖后2 min，酸排泄开始缓慢，并出现中间产物。还原型谷胱甘肽（NADH）含量先下降后上升。这些结果表明，厌氧S.变形杆菌细胞同时代谢葡萄糖和山梨糖醇，而在山梨糖醇存在下，好氧细胞可以在加入葡萄糖后2 min开始代谢葡萄糖，因为葡萄糖转运的中间产物（PEP电位）积累。少

项目成果

H.Miyasawa: "Growth-phase- and pH-dependent xylitol inhibition on acid production of Streptococcus mutans"Journal of Dental Research. 79(in press). (2000)

H.Miyasawa：“生长阶段和 pH 依赖性木糖醇对变形链球菌产酸的抑制”牙科研究杂志。

Y.Iwami et al.: "Intracellular flux of glucose metabolism in streptococcal cells by simultaneous monitoring of fluorescence dependent on reduced nicotinamide adenine nucleotide and acid excretion under strictly anaerobic conditions"Oral Microbiology and I

Y.Iwami 等人：“通过同时监测依赖于严格厌氧条件下烟酰胺腺嘌呤核苷酸和酸排泄的荧光的链球菌细胞内葡萄糖代谢通量”口腔微生物学和 I

H. Miyasawa et al.: "Growth-phase- and pH-dependent xylitol inhibition on acid production of Streptococcus mutans."Journal of Dental Research. 79(in press). (2000)

H. Miyasawa 等人：“生长阶段和 pH 依赖性木糖醇对变形链球菌酸产生的抑制。”牙科研究杂志。

宮澤はるみ: "Xylitol による mutans streptococci の酸産生阻害効果:増殖条件とpHの影響"東北大学歯学雑誌. 19(1)(印刷中). (2000)

Harumi Miyazawa：“木糖醇对变形链球菌产酸的抑制作用：生长条件和 pH 值的影响”东北大学牙科杂志 19(1)（印刷中）。

N.TAKAHASHI: "Acid-induced acid tolarance and acidogenicity of non-mutans streptococci" Oral Microbiology and Immunology. 14・1. 43-48 (1999)

N.TAKAHASHI：“非变形链球菌的酸诱导的耐酸性和产酸性”口腔微生物学和免疫学 14・1（1999）。