SEARCH AND POSSIBLE USAGE OF FUNCTIONAL MOLECULES USING COLLAGEN-FORMING CULTURED CELLS

使用胶原形成培养细胞搜索功能分子并可能使用

• 批准号: 10557208
• 负责人: OTSUKA Hideaki
• 金额: $ 4.67万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10557208/
• 关键词: collagen; M cell; Premna subscandens; acteoside; Alangium platanifolium; flavonoid; ヤエヤマハマクサギ; アクテオサイド

M cells derived rat liver were used for experiments. These cells have a unique feature that they are maintained as a strain. However, they form visible collagen fibers on prolonged cultivation under a presence of vitamin C. we conducted the isolation of an effector of collagen network formation by means of a bioassay-guided procedure.From n-BuOH-soluble fraction of the leave extract of Premna subscandens (Japanese name : Yaeyama-hamakusagi), collected in Okinawa, a phenylethanoid, acteoside was isolated as an effective compound which mutate the formation of collagen fiber networks formed by M cell. The compound made the M cell to form very minute networks which might be favorable on an occasion of remedy from wound due to rapid formation of granules. The investigation of structure and activity relationship, catechol moieties seemed to be required for both aglycone and ester portions. This compound is relatively well distributed in plant species and the contents in plants are generally high. This means that acteoside is available from natural sources.On the other hand, from the EtOAc-soluble fraction of leave extract of Alangium platamfolium var. platanlfolium (Japanese name : Momijiurinoki), collected in Fukuoka Pref., a flavonoid and phenylglycerol adduct was isolated as a promising compound to inhibit the formation of collagen fiber. The flavonoid portion was closely related to luteolin and the other part was 3-hydroxy-4-methoxyphenylglycerol. The activity was estimated to be at 2 μg/ml for complete inhibition. Other flavonoids with catechol-like functional group also exhibit some inhibitory activity.From these results, this method found to be used to search functional compounds to have the activity to inhibit or promote collagen fiber formation.

用大鼠肝M细胞进行实验。这些细胞具有独特的特征，即它们作为菌株维持。然而，它们在维生素C存在下长时间培养时形成可见的胶原纤维。从采集于冲绳的豆腐柴（日本名：八重山-滨草木）的叶提取物的n-BuOH可溶性部分中，分离出一种苯乙醇类化合物，类叶糖苷，作为一种使M细胞形成的胶原纤维网络的形成发生突变的有效化合物。该化合物使M细胞形成非常微小的网络，由于快速形成颗粒，这可能有利于治疗创伤的场合。结构与活性关系的研究表明，儿茶酚类结构对苷元和酯基都是必需的。该化合物在植物种中分布较好，植物中含量普遍较高。另一方面，从八角枫的叶提取物的乙酸乙酯可溶性级分中，可以获得毛蕊花糖苷。Platanlfolium（日本名：Momijiurinoki），收集于福冈县，类黄酮和苯基甘油加合物作为抑制胶原纤维形成的有希望的化合物被分离。黄酮部分与毛地黄黄酮密切相关，另一部分为3-羟基-4-甲氧基苯基甘油。对于完全抑制，活性估计为2 μg/ml。其他类黄酮类化合物也表现出一定的抑制活性，因此，本方法可用于寻找具有抑制或促进胶原纤维形成活性的功能化合物。

项目成果

Hirokazu Sudo, Kaori Kijima, Toshinori Ide, Hideki Otsuka, Eiji Hirata, Yoshio Takeda, Masayuki Isaji and Yoshikazu Kurashina: "A collagen network formation effector from leaves of Premna subscandens"Chemical and Pharmaceutical Bulletin. 47(9). 1341-1343

Hirokazu Sudo、Kaori Kijima、Toshinori Ide、Hideki Otsuka、Eiji Hirata、Yoshio Takeda、Masayuki Isaji 和 Yoshikazu Kurashina：“来自 Premna subscandens 叶子的胶原蛋白网络形成效应器”化学和药物通报。

Hirokazu Sudo: "Acollagen Network Formation Effector from Leaves of Premnasubscandens"Cehm.Pherm.Bull.. 47. 1341-1343 (1999)

Hirokazu Sudo：“来自 Premnasubscandens 叶子的胶原蛋白网络形成效应器”Cehm.Pherm.Bull.. 47. 1341-1343 (1999)

玉木昭江: "モミジウリノキのコラーゲン線維形成抑制成分の探索"日本生薬学会年会講演要旨集. 46. 193 (1999)

Akie Tamaki：“寻找日本枫树的胶原纤维形成抑制成分”日本草药药理学会年会记录 46. 193 (1999)。

Akie Tamaki, Toshinori Ide, Hideaki Otsuka: "Collagen fiber formation inhibition from leaves of Alangium Platanifolium var. platanifolium"Abstract papers of the 46th Annual Meeting of the Japanese Society of Pharmacognosy. 198 (1999)

Akie Tamaki、Toshinori Ide、Hideaki Otsuka：“Alangium Platanifolium v​​ar. platanifolium 叶中的胶原纤维形成抑制”日本生药学会第 46 届年会摘要论文。

玉木昭江: "モミジウリノキのコラーゲン線維形成抑制成分の探索"日本生薬学会年会 講演要旨集. 46. 193 (1999)

Akie Tamaki：“寻找苦瓜中抑制胶原纤维形成的成分”日本草药科学学会年会摘要 46. 193 (1999)。