SEARCH AND POSSIBLE USAGE OF FUNCTIONAL MOLECULES USING COLLAGEN-FORMING CULTURED CELLS

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M cells derived rat liver were used for experiments. These cells have a unique feature that they are maintained as a strain. However, they form visible collagen fibers on prolonged cultivation under a presence of vitamin C. we conducted the isolation of an effector of collagen network formation by means of a bioassay-guided procedure.From n-BuOH-soluble fraction of the leave extract of Premna subscandens (Japanese name : Yaeyama-hamakusagi), collected in Okinawa, a phenylethanoid, acteoside was isolated as an effective compound which mutate the formation of collagen fiber networks formed by M cell. The compound made the M cell to form very minute networks which might be favorable on an occasion of remedy from wound due to rapid formation of granules. The investigation of structure and activity relationship, catechol moieties seemed to be required for both aglycone and ester portions. This compound is relatively well distributed in plant species and the contents in plants are generally high. This means that acteoside is available from natural sources.On the other hand, from the EtOAc-soluble fraction of leave extract of Alangium platamfolium var. platanlfolium (Japanese name : Momijiurinoki), collected in Fukuoka Pref., a flavonoid and phenylglycerol adduct was isolated as a promising compound to inhibit the formation of collagen fiber. The flavonoid portion was closely related to luteolin and the other part was 3-hydroxy-4-methoxyphenylglycerol. The activity was estimated to be at 2 μg/ml for complete inhibition. Other flavonoids with catechol-like functional group also exhibit some inhibitory activity.From these results, this method found to be used to search functional compounds to have the activity to inhibit or promote collagen fiber formation.