Molecular mechanism of regulation of rice seed germination

水稻种子萌发调控的分子机制

基本信息

批准号：
10640628
负责人：
MITSUI Toshiaki
金额：
$ 2.24万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Aim of this research project is to clarify the molecular mechanism of germination of rice seeds. The results obtained in this research periods are as follow:1. The expression of α-amylase in the scutellar tissues and the aleurone layer of germinating rice seeds was differentially regulated by gibberellin (GA), abscisic acid (ABA), and metabolic sugars. The α-amylase expression in aleurone layer was induced by GA and the GA effect was suppressed by ABA, while the α-amylase expression in scutellum was strongly reduced by glucose and ABA but not ABA alone.2. Sucrose controlled the intracellular transport of α-amylase and stimulated its turnover in rice cells. Sugar also controlled the CaィイD12+ィエD1 uptake into rice cells, and there were a close relationship between the α-amylase expression and the CaィイD12+ィエD1 uptake.3. ABA stimulated the glucose uptake and sucrose synthesis in the scutellar tissues. It was also found that sucrose synthesis occurred in the aleurone layer, and that the sucrose synthesis was inhibited by GA and the GA effect was suppressed by ABA.4. The inositol 1,4,5-trisphosphate (IPィイD23ィエD2)-CaィイD12+ィエD1 signal transduction was suggested to be involved in the seed germination and the GA-inducible α-amylase expression. The level of IPィイD23ィエD2 in the aleurone layer was increased by GA. GA also induced the inositol phospholipid-specific phospholipase C in the aleurone.Overall results indicated that the close talk of GA, ABA and metabolic sugars is involved in the regulation of α-amylase expression and germination of rice seed.

该研究项目的目的是阐明水稻种子发芽的分子机制。在本研究期间获得的结果如下：1。 gibberellin（GA），脱甲酸（ABA）和代谢糖在scutellar组织中α-淀粉酶的表达和发芽水稻种子的α-淀粉酶层的表达不同。 GA诱导α-淀粉酶层中的α-淀粉酶表达，而ABA抑制了GA效应，而Scutellum中的α-淀粉酶表达则被葡萄糖和ABA强烈降低，但单独降低了ABA。蔗糖控制了α-淀粉酶的细胞内转运，并刺激了其在水稻细胞中的周转率。糖还控制了CAIY D12+IY D1对水稻细胞的摄取，并且α-淀粉酶表达与CAIY D12+IY D1摄取之间存在密切的关系。3。 ABA刺激了危险组织中的葡萄糖摄取和蔗糖合成。还发现蔗糖合成发生在核龙层中，并且蔗糖合成被GA抑制，而GA效应被ABA.4抑制。肌醇1,4,5-三磷酸（IPII D23 E D2）-CAI D12+E D1信号转导，建议与种子发芽和可诱导的α-淀粉酶表达有关。 GA增加了核龙层中的IPI D23 E D2的水平。 GA还诱导了Aleurone中的肌醇磷脂特异性磷脂酶C。反到所有结果表明，GA，ABA和代谢糖的紧密讨论参与调节α-淀粉酶表达和水稻种子的发芽。