Analysis of starch-to-sucrose transition mechanism in germinating rice seed and its application

水稻种子发芽过程中淀粉-蔗糖转化机理分析及其应用

基本信息

批准号：
12660071
负责人：
MITSUI Toshiaki
金额：
$ 2.24万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Aim of this research project is to clarify the starch-to-sucrose transition mechanism in germinating rice seed and to establish control system of seed germination. The results obtained in this research period are as follow:1. Temporal, special and hormone-regulated expression of α-amylase and UDP-glucose pyrophosphorylase (UGPase) in germinating rice seeds: UGPase was concluded to be house-keeping enzyme in the scutellar and aleurone tissues. Temporal and spatial expressions of α-amylase I-1 and II-4 were essentially identical in the aleurone layer, although these were distinguishable in the embryo tissues at the early stage of germinatioa It was found that the post-transcriptional regulation of expression of α-amylase II-4, that lacks the gibberellin (GA) response cis-element in the promoter region of the gene, by GA operates in the aleurone layer of germinating rice seed, which is mediated by Ca^<2+> and calmodulin.2. Involvement of histone acetylation in transcription of α-amylase genes: A potent inhibitor for histone deacetylase, Trichostatin A increased the level of α-amylase mRNA in the aleuome without GA, suggesting that the histone acetylation is involved in the regulation of α-amylase gene expression.3. Proteome analysis of scuteller tissues: In the scutellar tissues of germinating rice seeds, several proteins that exhibited the time-dependent and abscisic acid (ABA)-inducible expression were listed up. Glycolitic enzymes, such as phosphoglycerate mutase, glyceraldehydes-3P dehydrogenase, etc., were identified in these proteins. GS-129, one of the listed proteins exhibited the following characteristics: (a) GS-129 tightly associated with cell wall materials, (b) GS-129 was glycoprotein bearing both N-linked and O-linked saccharide chains, (c) GS-129 was suggested to be a possible xylan hydrolyzing enzyme.

该研究项目的目的是阐明稻米种子发芽中的淀粉到核酸糖过渡机制，并建立种子发芽的控制系统。在本研究期间获得的结果如下：1。在发芽的水稻种子中，α-淀粉酶和UDP-葡萄糖焦磷酸化酶（UGPase）的时间，特殊和同性调节的表达：the-ugpase：ugpase the the Theshess the the Cugpase thestud castudud the是在Scutellar和Aleurone组织中的家务酶。 Temporal and spatial expressions of α-amylase I-1 and II-4 were essentially identical in the aleurone layer, although these were distinguishable in the embryo tissues at the early stage of germinatioa It was found that the post-transcriptional regulation of expression of α-amylase II-4, that lacks the gibberellin (GA) response cis-element in the promoter region of the gene, by GA operates in the发芽稻种子的核酮层，由Ca^<2+>和钙调蛋白介导。2。组蛋白乙酰化参与α-淀粉酶基因的转录：组蛋白脱乙酰基酶的潜在抑制剂，trichostatin a增加了无ga中烯烃中α-淀粉酶mRNA的水平，这表明组蛋白乙酰化参与了α-氨基酶基因表达的调节。3。 scutellar组织的蛋白质组分析：在发芽的水稻种子的scutellar组织中，列出了几种暴露了时间依赖性和脱落酸（ABA）诱导表达的蛋白质。在这些蛋白质中鉴定出糖溶酶，例如磷酸甘油酸突变酶，甘油醛-3p脱氢酶等。 GS-129，列表的蛋白质之一暴露了以下特征：（a）与细胞壁材料紧密相关的GS-129，（b）GS-129是糖蛋白具有N-连接和O-链路连接的糖糖链的糖蛋白，（C）GS-129可能是Xylan Hypololysyseme的可能性。